Expression of G1 Phase Cyclins in Human Gastric... : Digestive Diseases & Sciences (original) (raw)

Gastrointestinal Oncology

Expression of G1 Phase Cyclins in Human Gastric Cancer and Gastric Mucosa of First-Degree Relatives

Stephan Miehlke
Jun Yu
Matthias Ebert
Daniel Szokodi
Michael Vieth
Eberhard Kuhlisch
Rene Buchcik
Wolfgang Schimmin
Ursula Wehrmann
Peter Malfertheiner
Gerhard Ehninger
Ekkehard Bayerdörffer
Manfred Stolte

Digestive Diseases & Sciences

47(6):p 1248-1256, June 2002.

The aim of this study was to investigate the expression of D-type cyclins and cyclin E in gastric cancer patients (N = 34), in healthy first-degree relatives of gastric cancer patients (N = 29), and in control subjects (N = 18). Expression of cyclins D1, D2, D3, and E was determined by RT-PCR. Localization of cyclin expression was determined by immunohistochemistry. Expression of cyclins D2, D3, and E was more frequently detected in tumor tissue compared with tumor-free gastric mucosa (P < 0.05) and was associated with the presence of intestinal metaplasia. In contrast, cyclin D1 was frequently expressed in both tumor- and tumor-free tissue. Cyclin D3 expression was more frequently detected in the antrum mucosa of first-degree relatives compared to controls (P < 0.01) and was associated with the presence of Helicobacter pylori. Our data suggest that deregulation of G1 phase cyclins may play a role in gastric carcinogenesis, and may point to the presence of molecular alterations in individuals at an increased risk for gastric cancer.