A soluble nonglycosylated recombinant infectious... : Fish & Shellfish Immunology (original) (raw)

A soluble nonglycosylated recombinant infectious hematopoietic necrosis virus (IHNV) G-protein induces IFNs in rainbow trout ( Oncorhynchus mykiss )

• Noel Verjan
• Ei Lin Ooi
• Tomonori Nochi
• Hidehiro Kondo
• Ikuo Hirono
• Takashi Aoki
• Hiroshi Kiyono
• Yoshikazu Yuki

Fish & Shellfish Immunology

25

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1

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:p

170

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180

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July 2008

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| DOI: 10.1016/j.fsi.2008.04.004

Abstract

Viral glycoproteins interact with cell-surface receptors to mediate virus entry and innate immune system activation. We found that a soluble recombinant infectious hematopoietic necrosis virus G-protein (rIHNV-G) stimulated an early innate immune response mediated by proinflammatory cytokines, IFN1 and IFN-γ in rainbow trout (Oncorhynchus mykiss) fry. Expression of both IFN1 and IFN-γ mRNA transcripts was an early event and was rIHNV-G dose-dependent. In addition, preliminary evidence revealed that the innate immune response induced by rIHNV-G protein could protect rainbow trout fry from a subsequent IHNV virus challenge. Finally, the binding and distribution of FITC-rIHNV-G protein on rainbow trout spleen and head kidney leukocytes resemble morphological changes which occur on the cell membrane during antigen–receptor interaction including membrane reorganization, patching, polarization and capping. Thus a soluble nonglycosylated rIHNV-G protein could mediate the activation of rainbow trout leukocytes, with concomitant production of proinflammatory cytokines and IFNs.

Copyright © 2008Elsevier, Inc.