Effect of tissue fixation on... : Journal of Histochemistry & Cytochemistry (original) (raw)

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Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.

• B Schutte
• M M Reynders
• F T Bosman
• G H Blijham

Journal of Histochemistry & Cytochemistry

35

(

11

)

:p

1343

-

1345

,

November 1987

.

| DOI: 10.1177/35.11.3116075

We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.