Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor (original) (raw)

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• Authors:

  • Soetkin Versteyhe
  • Christophe Blanquart
  • Cornelia Hampe
  • Shaukat Mahmood
  • Nevena Christeff
  • Pierre De Meyts
  • Steven G. Gray
  • Tarik Issad

• View Affiliations
  Affiliations: Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, Université Libre de Bruxelles, 1070 Brussels, Belgium. soetkinversteyhe@gmail.com

• Published online on: January 1, 2010 https://doi.org/10.3892/mmr\_00000239

• Pages: 189-193

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Abstract

Insulin receptor substrates (IRS)-5 and -6 are two recently identified members of the IRS family. We investigated their roles as insulin receptor substrates and compared them with Src-homology-2-containing (Shc) protein, a well-established substrate. Bioluminescence resonance energy transfer (BRET) experiments showed no interaction between the receptor and IRS-5, while interaction with IRS-6 was not enhanced by insulin. By contrast, Shc showed an insulin-induced BRET response, as did a truncated form of IRS-1 (1-262). While Shc was heavily phosphorylated after stimulation of the insulin receptor, IRS-5 and -6 showed very weak phosphorylation levels. These results suggest that, although these two adaptors have previously been proposed as substrates for the insulin receptor, they are poor substrates for the insulin receptor. This calls into question their relevance to insulin signalling.

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• Impact Factor: 3.4
• Ranked Medicine Research and Experimental
  (total number of cites)
• CiteScore: 7.6
• CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
• Source Normalized Impact per Paper (SNIP): 0.647
• SCImago Journal Rank (SJR): 0.781

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Spandidos Publications style

Versteyhe S, Blanquart C, Hampe C, Mahmood S, Christeff N, De Meyts P, Gray SG and Issad T: Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor . Mol Med Rep 3: 189-193, 2010.

APA

Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P. ... Issad, T. (2010). Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor . Molecular Medicine Reports, 3, 189-193. https://doi.org/10.3892/mmr\_00000239

MLA

Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P., Gray, S. G., Issad, T."Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor ". Molecular Medicine Reports 3.1 (2010): 189-193.

Chicago

Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P., Gray, S. G., Issad, T."Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor ". Molecular Medicine Reports 3, no. 1 (2010): 189-193. https://doi.org/10.3892/mmr\_00000239