Dieckmann CL, et al. (1984) (original) (raw)

Reference: Dieckmann CL, et al. (1984)

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Abstract

Noncomplementing mutations in a nuclear gene (CBP1) of Saccharomyces cerevisiae D273-10B specifically affect the synthesis of cytochrome b, a mitochondrially encoded carrier of the respiratory chain. The nuclear mutants have been shown to have lowered levels of cytochrome b-specific transcripts. This phenotype is attributed to the inability of the mutant strains to process the 5' end of the cytochrome b pre-mRNA. Impairment of the processing function encoded by the CBP1 gene introduces an instability in the transcripts and promotes nucleolytic degradation. Mutations in CBP1 can be suppressed by a p- genome in which the 5' untranslated leader of the oli1 gene (subunit 9 of the ATPase) is fused near the 5' side of the cytochrome b coding sequence. The rearranged genome allows the cytochrome b gene to be transcribed from the oli1 promoter and results in novel cytochrome b transcripts with the 5' leader sequence of the oli1 mRNA. The presence of the oli1 leader sequence confers stability to the RNA and circumvents the CBP1 processing function.

Reference Type

Comparative Study | Journal Article | Research Support, U.S. Gov't, P.H.S.

Authors

Dieckmann CL, Koerner TJ, Tzagoloff A

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