Jelili Badmus | Ladoke Akintola Univeristy of Technology, Ogbomoso (original) (raw)

Papers by Jelili Badmus

Separations

Diabetes mellitus (DM) is a group of systemic metabolic disorders with a high rate of morbidity a... more Diabetes mellitus (DM) is a group of systemic metabolic disorders with a high rate of morbidity and mortality worldwide. Due to the detrimental side effects of the current treatment, there is a great need to develop more effective antidiabetic drugs with fewer side effects. Natural products are a well-known source for the discovery of new scaffolds for drug discovery, including new antidiabetic drugs. The genus Helichrysum has been shown to produce antidiabetic natural products. In this investigation, the methanolic extract of H. cymosum and H. pandurifolium resulted in the isolation and identification of eleven known compounds viz 5,8-dihydroxy-7-methoxy-2-phenyl flavanone (1), pinostrobin (2), dihydrobaicalein (3), glabranin (4), allopatuletin (5), pinostrobin chalcone (6), helichrysetin (7), 5-hydroxy-3,7-dimethoxyflavone (8), 3,5-dihydroxy-6,7,8-trimethoxyflavone (9), 3-O-methylquercetin (10), and 3-methylethergalangin (11). The in vitro bio-evaluation of isolated compounds agai...

Caryologia

Nanotechnology is widely gaining worldwide application in biology and medicine because of its pro... more Nanotechnology is widely gaining worldwide application in biology and medicine because of its proven efficacy. Annona muricata contains bioactive phytochemicals with an inherent ability to bio-fabricate metal ions nanoparticles (NPs). Annona muricata aqueous leaf extract and its green bio-fabricated silver nanoparticles were evaluated on red blood cells (RBC) for anti-haemolytic activity and cytogenotoxicity on Allium cepa cells. The effects of A. muricata extract (Am-E) and its biofabricated silver nanoparticles (Am-AgNPs) were observed at 0.7, 7.0 and 70.0 µg/ml on H2O2-induced haemolysis in RBC and cyclophosphamide-induced cytogenotoxicity on A. cepa cells. Results showed significant and concentration dependent anti-haemolytic activity of Am-E relative to Am-AgNPs. Significant (P<0.05) reduction of mitotic index was observed in the groups treated with Am-AgNPs compared with Am-E, which indicates cytotoxic effect of the nanoparticles. The Am-E protected A. cepa meristem root ce...

Nano Plus: Science and Technology of Nanomaterials, 2021

Nanoparticles have been proven to have a wide range of applications due to their peculiar propert... more Nanoparticles have been proven to have a wide range of applications due to their peculiar properties. Of the metallic nanoparticles, green synthesized silver nanoparticles (AgNPs) have been widely used in various applications. This study evaluates the potentials of corn silk extract in the synthesis of silver nanoparticles (CS-AgNPs). The biological activities are tested through various applications as well as their safety. Powdered corn silk (1g) was heated in 100 ml of distilled water at 60°C for 1h, cooled, and centrifuged at 4000 rpm for 30 minutes. Then, 1ml of the extract was added to 40ml of 1mM AgNO3 to reduce Ag+ to nano size. Characterization of CS-AgNPs was through UV-visible spectroscopy, Fourier-transform-infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Energy Dispersive X-ray (EDX) analysis. The efficacy of CS-AgNPs as a larvicidal, anticoagulant, thrombolytic, and antioxidant agent was evaluated. Safety evaluation was carried out using Allium ...

Resistance to artemisinins has led to possible anti malarial combination therapy. This study was ... more Resistance to artemisinins has led to possible anti malarial combination therapy. This study was undertaken to investigate the effects of co - administration of artesunate and Azadirachta indica leaves extract on antioxidants and certain liver parameters in rats. 24 male rats weighing averagely 190g were randomly selected into four groups, administered therapeutic doses of artesunate and ext ract namely : Group A (Control), Group B (Extract only)), Group C (Artesunate only) and Group D (Artesunate with extract). Total protein, Cholesterol concentrations, Alanine amino transferase (ALT), Alkaline phosphatase (ALP) and Gamma glutamyl transferase (GGT) activities were determined using international standardized methods. Malondialdehyde, (MDA), reduced glutathione (GSH), phenols, flavonoids, hydroxyl (OH · ) and 1 - 1 - diphenyl - 2 - picryl hydrazyl (DPPH) radicals scavenging effects were determined spectr ophotometrically. Results obtained showed significant increases (P ≤0.05) in pla...

Evidence-Based Complementary and Alternative Medicine, 2020

IOP Conference Series: Materials Science and Engineering, 2020

Animal waste materials are rarely used in the synthesis of nanoparticles compared to microorganis... more Animal waste materials are rarely used in the synthesis of nanoparticles compared to microorganisms and plant materials. The use of animal fur (goat) in synthesis could assist in turning waste to wealth. Thus, potentials of animal fur in the synthesis of silver nanoparticles (AF-AgNPs), its biological activities and safety through cytogenotoxicity were investigated. Animal fur (1 g) was hydrolyzed with 100 ml of 0.1 M NaOH at 90 °C for 1 h, cooled and centrifuged at 4000 rpm for 30 min. The extract (1 ml) was added to 1 mM AgNO3 (40 ml) to reduce Ag+ to its nanoparticles. The AF-AgNPs was characterized using UV–vis spectroscopy, Fourier-transform-infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Energy Dispersive X-ray (EDX) analysis. Larvicidal, antioxidant, anticoagulant and thrombolytic potentials of AF-AgNPs were studied. Onion bulbs (20) were exposed to 0.01, 0.10, 1.0, 10.0 and 100.0 μg/ml of AF-AgNPs solution for its cytogenotoxicity study with AgNO3 so...

IOP Conference Series: Materials Science and Engineering, 2020

Research work on corrosion behaviour of metals and their alloys continues to attract considerable... more Research work on corrosion behaviour of metals and their alloys continues to attract considerable attention because of the huge cost required to prevent corrosion and replace failed or contaminated components due to corrosion reactions. Different inhibitors have been used to minimize corrosion rate, but addition of nanoparticles has been reported to enhance inhibition efficiency. In this work, the influence of silver nanoparticles (AgNPs) on inhibition of corrosion of mild steel, stainless steel and aluminum in 1.0 M HCl were investigated. Biosynthesized AgNPs were acquired from Laboratory of Industrial Nanobiotechnology, Ladoke Akintola University of Technology Ogbomoso. The particles were hitherto characterized using FTIR, UV-Vis and TEM. Five concentrations of AgNPs solution (0, 5, 10, 15, and 20 μg/ml) were added to 1.0 M HCl. The corrosion inhibition effects of the AgNPs on the metal samples were analyzed using gravimetric (weight loss), potentiodynamic polarization and gasomet...

Nanotechnology for Environmental Engineering, 2019

While several green biomolecules have been used to synthesize functional and biologically compati... more While several green biomolecules have been used to synthesize functional and biologically compatible nanoparticles, little attention has been paid to Persea americana (PA) (avocado) fruit extract as a potential reducing agent. This study used avocado fruit peel aqueous extracts to synthesize silver nanoparticles (PAAgNPs), gold nanoparticles (PAAuNPs) and bimetallic alloy nanoparticles (PAAg-AuNPs). The particles were characterized using UV-vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy, selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy and X-ray diffraction among others. The particles were assessed for their antibacterial, antifungal and antioxidant properties. The UV-vis spectroscopy showed PAgNPs, PAAuNPs and PAAg-AuNPs with surface plasmon resonance at 455.5, 538 and 540.5 nm, respectively. The significant FTIR peaks: PAAgNPs (3358 cm −1), PAAuNPs (3503 cm −1) and PAAg-AuNPs (3651 cm −1) pointed to protein as both capping and stabilizing agents for the synthesized nanoparticles. Generally, the particles were spherical, with size range of 18-80 nm for PAAgNPs, 16-71 nm for PAAuNPs and 44-55 nm for PAAg-AuNPs. The energy-dispersive X-ray spectra showed silver, gold and silver/gold as conspicuous metals in PAAgNPs, PAAuNPs and PAAg-AuNPs colloids, respectively. SAED showed ring-shaped patterns for the particles. The nanoparticles effectively inhibited growth of tested bacteria (11-94%) for PAAgNPs, (10-77%) for PAAuNPs and (20-85%) for PAAg-AuNPs. The effectiveness of the biosynthesized nanoparticles can be placed as PAAg-AuNPs > PAAgNPs > PAAuNPs. The fungal inhibition performances are 33-76%, 50-82% and 27-88% for PAAgNPs, PAAuNPs and PAAg-AuNPs, respectively, while DPPH-scavenging activities were 57.82-63.25%, 15.28-54.50% and 53.05-54.26%, which were dose dependent at the tested concentrations of 20-100 µg/ml with good antioxidant activities compared to standard BHA (41.46-84.57%) and ascorbic acid (43.56-91.10%). The bleaching inhibition assay of ABTS showed activities of 56.15-85.43% (PAAgNPs), 34.67-50.93% (PAAuNPs) and 45.31-94.01% (PAAg-AuNPs). The lower concentrations of EC 50 were obtained in nanoparticles (24.45-58.33 µg/ml) compared with the standards (38.42-69.04 µg/ml), indicating that the nanoparticles could suffice as good agents in drug consignment. This study has demonstrated the potential of P. americana fruit peel aqueous extracts to synthesize AgNPs, AuNPs and Ag-AuNPs for antimicrobial and antioxidant applications. The current work, to the best of our knowledge, is the first to use avocado peel extract to synthesis nanoparticles.

Asian Pacific Journal of Health Sciences, 2016

Gentamicin have been shown to generate free radicals .This study investigated modulatory potentia... more Gentamicin have been shown to generate free radicals .This study investigated modulatory potential of aqueous extract of Hibiscus Sabdariffa calyx with gentamicin in rats. Thirty rats weighing between 150-200g were randomly selected into 6 groups: Group A( control), group B received 200mg/kg body weight of extract, group C received 80mg/ kg body weight of gentamicin intra peritoneally for six days while group D, E and F received 200mg/kg, 400mg/kg and 600mg/kg body weight of extract for six days before and eight days following administration of 80mg/kg gentamicin. Antioxidants and biochemical indices were determined using standardized methods . Rats treated with extracts at 200mg/kg elicit significant (p<0.05) decreases in total bilirubin (TB), conjugated bilirubin (C.BIL), urea, creatinine, Malondialdehyde (MDA) concentrations, Aspartate aminotransferase (AST), Alanine aminotansferase (ALT) and Alkaline phosphatase (ALP) activities as well as significant( p<0.05) increases in Superoxide Dismutate (SOD) activity , levels of reduced glutathione (GSH) ,albumin and total protein (TP). Gentamicin treated rats however showed significant (p<0.05) decreases in GSH, TP, albumin and SOD activities with elevated levels of MDA, TB,C.BIL, urea, creatinine and AST, ALT and ALP activities . Furthermore , animals treated with various concentrations of extract after gentamicin administration showed significant increases (p<0.05) in concentrations of TP, ALB, GSH and SOD activities with corresponding decreases in TB, C.BIL, urea, creatinine and MDA concentrations as well as AST,ALT and ALP activities compared with gentamicin group in a dose dependent manner. Properties exhibited by extract is antioxidative suggestive of its modulatory effects on gentamicin activity .

Advances in Life Science and Technology, 2019

Arsenic is an environmental pollutant and its contamination in the drinking water is considered a... more Arsenic is an environmental pollutant and its contamination in the drinking water is considered as a serious worldwide environmental health threat. The present study investigated the effects of arsenic exposure on antioxidant parameters and p53 expression in male albino rats. The animals (n=45) were exposed to arsenic (100 ppm, 150 ppm and 200 ppm) for 4, 8 and 12 weeks as sodium arsenate in drinking water. Control animals (n=15) received distilled water for the same period. Hepatic superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), reduced glutathione (GSH), lipid peroxidation and total protein were evaluated spectrophotometrically. Expression of p53 was also detected by histochemical staining. Before the commencement of arsenic exposure, five animals were sacrificed to obtain baseline data. ANOVA followed by Tukey's test was used to analyse the results with p<0.05 considered significant. Significant decrement in hepatic activities of SOD, catalase and GPx as well as hepatic concentration of GSH and total protein concentration characterized exposure to all the dose regimens of inorganic arsenic at all the time interval. Corroboratively, significant elevation was observed in malondialdehyde (MDA). The expression of p53 decreased in the groups that were exposed to arsenic as compared to the control animals. The findings from the present study suggests excessive generation of free radicals and reduction in p53 expression in arsenic-induced hepatotoxicity.

Aluminium phosphide is an oral rodenticide and insecticide that is toxic to multiple organs in no... more Aluminium phosphide is an oral rodenticide and insecticide that is toxic to multiple organs in non-target organisms with no known antidote. This study investigated aluminium phosphide-induced hematotoxicity and ovarian oxidative damage in rat and evaluated the effectiveness of hesperidin as a therapeutic agent against the toxic effects. Rats receiving a sub-lethal dose of aluminium phosphide (1.15 mg kg-1 body weight) for 30 days, exhibited significant impaired hematological parameters with distorted leucocyte and thrombocytic indices. Aluminium phosphide exposure produced macrocytic, hyperchromic anemia. The toxicity also included a reduction in the white blood cell, lymphocyte, and granulocyte counts. Ovarian oxidative stress indicators comprising malonaldehyde and lipid hydroperoxides levels were markedly increased, while the antioxidant enzymatic system was inhibited. Co-treatment with hesperidin ameliorated the aluminum phosphide-induced anemia, thrombocytopenia and leukopenia ...

Journal of Pharmacology and Toxicology, 2012

Nano Plus: Science and Technology of Nanomaterials, 2021

The Nanotechnology Research Group (NANO+) at Ladoke Akintola University of Technology, Ogbomoso, ... more The Nanotechnology Research Group (NANO+) at Ladoke Akintola University of Technology, Ogbomoso, Nigeria organized her 5th international conference on nanotechnology on 26-28 October 2021 using both physical and Zoom platforms (hybrid). The conference coded ‘LAUTECH NANO 2021’ with the theme ‘Nanotechnology in Africa: Changing the Narratives for the Benefits of Mankind’ provided a platform for African nations to explore nanotechnology to engender the much-desired growth and development on the continent, and to also bridge the gap of nanotechnology divide between Africa and the developed nations. In this regard, active players and seasoned scholars in nanotechnology R&D in Africa and other countries shared their experiences and visions with participants. In addition, fifty-seven (57) papers were presented at the technical sessions in diverse areas of science, engineering, agriculture, and biomedicine from 45 institutions/agencies in more than 10 countries. This book of abstracts cont...

BMC Complementary and Alternative Medicine, 2019

Background: The plant Holarrhena floribunda (H. floribunda; G. Don) is indigenous to sub-Saharan ... more Background: The plant Holarrhena floribunda (H. floribunda; G. Don) is indigenous to sub-Saharan Africa and is traditionally used to treat several ailments. The present study was carried out to isolate and characterize bioactive compounds with anti-proliferative activity present in H. floribunda extracts. Methods: Compounds were isolated from H. floribunda using the bioassay-guided fractionation technique of repeated column chromatography and the step-wise application of the MTT reduction assay to assess antiproliferative bioactivity. The structures of the compounds were identified mainly using NMR. The effects of the isolated compounds on the viability, cell cycle and proliferation of human cancer cell lines (MCF-7, HeLa and HT-29) as well as the non-cancerous human fibroblast cell line (KMST-6) were investigated. Results: Bioassay-guided fractionation yielded two steroidal alkaloids: holamine (1) and funtumine (2). The MTT reduction assay shows that both compounds exhibited selective dose-dependent cytotoxicity against the cancer cell lines studied. The isolated compounds induced cell cycle arrest at the G 0 /G 1 and G 2 /M phases in the cancer cell lines with significant reduction in DNA synthesis. The results obtained show that the cancer cells (MCF-7, HeLa and HT-29) used in this study were more sensitive to the isolated compounds compared to the noncancerous fibroblast cells (KMST-6). Conclusion: The ability of the isolated compounds to cause cell cycle arrest and reduce DNA synthesis raises hopes for their possible development and use as potent anticancer drugs. However, more mechanistic studies need to be done for complete validation of the efficacy of the two compounds.

Journal of Natural Sciences Research, 2019

Arsenic exposure has been implicated by several epidemiological studies as an important metalloid... more Arsenic exposure has been implicated by several epidemiological studies as an important metalloid that is currently poisoning millions of people globally. In order to investigate the time-course of arsenic exposure on hepatic and renal toxicity, male albino rats (n=45) were exposed to arsenic (100 ppm, 150 ppm and 200 ppm) for 4, 8 and 12 weeks as sodium arsenate in their drinking water. Control animals (n=15) received distilled water for the same period after which blood and vital organs were removed from the animals and analyzed for alanine amino transaminase (ALT), aspartate amino transaminase (AST) gamma amino transaminase (γGT), alkaline phosphatase (ALP), creatinine and urea spectrophotometrically. Histological changes in hepatocytes was also examined. Before the commencement of arsenic exposure, five animals were sacrificed to obtain baseline data. Significant elevation in plasma ALT, AST, γGT and alkaline phosphatase activities characterized the effect of the arsenical at all doses and time interval relative to the controls. Plasma levels of creatinine and urea were also elevated at all-time intervals in the arsenic group. In most of the cases observed, the elevated level of these biochemical marker in circulation are time-and dose-dose dependent. Hepatic histopathology reveals degeneration of cytoplasmic contents, evidence of necrosis, collapse of central vein, cytoplasmic inclusion and enlarged hepatic sinusoids in arsenic-exposed groups. These findings suggest that different dose regimens of sodium arsenate at different time interval caused degenerative changes in hepatic and renal tissues in rats in dose-and time-dependent fashion.

Antineoplastic drugs are well known for their abilities to induce varying degrees of toxicities a... more Antineoplastic drugs are well known for their abilities to induce varying degrees of toxicities and predispose to cardiovascular related disease states. This study, therefore evaluates the effects of Doxorubicin (Dox) (3 mg/kg body weight) and Etoposide (Etop) (3 mg/kg body weight) alone and in combinations at 12 and 24 h intervals on plasma lipid profiles and paraoxonase-1. Lipid peroxidation, superoxide dismutase (SOD), glutathione peroxidase (Gpx) and reduced glutathione (GSH) in selected male rat organs were also evaluated. The results were analyzed using One-way ANOVA followed by post hoc tests. Hypocholesterolemia characterized the effects of Etop alone and in combination with Dox at 12 h, while at 24 h interval hypercholesterolemia was observed in the combined group. Both drugs induced significant hypertriglyc-eridemia except at 12 h combination with significant hypotriglyceridemia. Individually , the drugs significantly reduced HDL-cholesterol (HDL-C) contrarily to up-regula...

Journal of Hazardous Materials Advances, 2022

Arsenic is a naturally occurring and ubiquitously present metalloid in soil, water, food, and t... more Arsenic is a naturally occurring and ubiquitously present metalloid in soil, water, food,
and the environment. Arsenic exposure to human through various sources such as contaminated groundwater and other human activities have become a significant global concern. This is because arsenic has been shown to exhibit extreme toxic
potential with serious health implications. Its exposure to humans is linked to myriads of disorders and this represents a major threat to the health, economic, and social well-being of people, especially, in the less-developed countries of the world. Major diseases that have been linked to arsenic poisoning are diabetes, hyperkeratosis,
cancer, hypertension, and neurodegeneration, etc. Meanwhile, to date, there is no specific or approved remedy for arsenic poisoning. Chelation therapy for the management of arsenic poisoning is fraught with several side effects which limit its clinical usefulness. In this review article, we tried to provide an insight into some
of the key scientific details that are documented in the literature as regards arsenic toxicity. These include its physicochemical properties, means of exposure, and possible mechanism of arsenic-induced toxicity. Furthermore, we also beamed our searchlight on the effect of arsenic on some major organs of the body. Online search
Engines such as Google®, Scholar google®, SciFinder®, and PubMed® were used for literature search. Scientific journals that relate to the objective of this review without restriction to the year of publication were considered. This study revealed the significance of arsenic toxicity and its contribution to health-related challenges.
Therefore, it is pertinent for policy Makers to increase awareness of arsenic toxicity and formulate guidelines for a cleaner environment. This will significantly reduce the incidence of arsenic-induced non-communicable
diseases

Oxidative damage is a factor in human diseases. In this study, effects of extract and fractions o... more Oxidative damage is a factor in human diseases. In this study, effects of extract and fractions of Solanium aethiopicum leaves on certain liver function indices, anti oxidative and anti-lipid peroxidation potentials were investigated. Phenols, flavonoids, antioxidative potentials, enzyme assays were determined spectrophotometrically using standardized methods. Results showed rats fed methanol extract (ME) at 50, 100, 150 and 200 mg/kg body weight for 21 days showed significant (P<0.05) decreases in liver Malondialdehyde and cholesterol concentrations by 2.530±0.050, 1.780±0.020, 1.670±0.030, 1.480±0.040 mg/ml protein and 69.290±2.660, 46.980±2.500, 39.030±4.820, 34.650±1.660 mg/dl respectively. At these concentrations, ME exhibited significant (p<0.05) decreases in serum Gamma glutamyl transferase (GGT) activity by 3.810 ± 0.010, 2.380 ± 0.010, 2.020 ± 0.010 and 1.190±0.010 IU/L compared with controls (3.830 ± 0.020 IU/L). Phenols and flavonoids of ME, Ethyl acetate fraction (...

Acta Biochimica Polonica

Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxyg... more Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxygen species (ROS). ROS oxidize cellular fatty acids to produce lipid peroxyl radicals, subsequently transformed into lipid peroxides, which decrease membrane fluidity and increase the activity of various enzymes implicated in degenerative diseases and cancer formation. Edible plants that contain exogenous compounds like curcumeroid, β-carotene, turmeric, and so on, protect the aerobic cells from oxidation of free radicals. This study thus evaluates antioxidant and antimutagenic activities of ethyl acetate, aqueous and methanolic fractions of Holarrhena floribunda leaves. Inhibitory activities of the ethyl acetate fraction on Fe(2+)-induced lipid peroxidation in hen egg yolk; rat liver and brain tissues were also evaluated. The Allium cepa root assay was used to evaluate antimutagenic activity. Results showed that the ethyl acetate scavenged DPPH, OH•, and •O2(-) much stronger than other f...

Separations

Diabetes mellitus (DM) is a group of systemic metabolic disorders with a high rate of morbidity a... more Diabetes mellitus (DM) is a group of systemic metabolic disorders with a high rate of morbidity and mortality worldwide. Due to the detrimental side effects of the current treatment, there is a great need to develop more effective antidiabetic drugs with fewer side effects. Natural products are a well-known source for the discovery of new scaffolds for drug discovery, including new antidiabetic drugs. The genus Helichrysum has been shown to produce antidiabetic natural products. In this investigation, the methanolic extract of H. cymosum and H. pandurifolium resulted in the isolation and identification of eleven known compounds viz 5,8-dihydroxy-7-methoxy-2-phenyl flavanone (1), pinostrobin (2), dihydrobaicalein (3), glabranin (4), allopatuletin (5), pinostrobin chalcone (6), helichrysetin (7), 5-hydroxy-3,7-dimethoxyflavone (8), 3,5-dihydroxy-6,7,8-trimethoxyflavone (9), 3-O-methylquercetin (10), and 3-methylethergalangin (11). The in vitro bio-evaluation of isolated compounds agai...

Caryologia

Nanotechnology is widely gaining worldwide application in biology and medicine because of its pro... more Nanotechnology is widely gaining worldwide application in biology and medicine because of its proven efficacy. Annona muricata contains bioactive phytochemicals with an inherent ability to bio-fabricate metal ions nanoparticles (NPs). Annona muricata aqueous leaf extract and its green bio-fabricated silver nanoparticles were evaluated on red blood cells (RBC) for anti-haemolytic activity and cytogenotoxicity on Allium cepa cells. The effects of A. muricata extract (Am-E) and its biofabricated silver nanoparticles (Am-AgNPs) were observed at 0.7, 7.0 and 70.0 µg/ml on H2O2-induced haemolysis in RBC and cyclophosphamide-induced cytogenotoxicity on A. cepa cells. Results showed significant and concentration dependent anti-haemolytic activity of Am-E relative to Am-AgNPs. Significant (P<0.05) reduction of mitotic index was observed in the groups treated with Am-AgNPs compared with Am-E, which indicates cytotoxic effect of the nanoparticles. The Am-E protected A. cepa meristem root ce...

Nano Plus: Science and Technology of Nanomaterials, 2021

Nanoparticles have been proven to have a wide range of applications due to their peculiar propert... more Nanoparticles have been proven to have a wide range of applications due to their peculiar properties. Of the metallic nanoparticles, green synthesized silver nanoparticles (AgNPs) have been widely used in various applications. This study evaluates the potentials of corn silk extract in the synthesis of silver nanoparticles (CS-AgNPs). The biological activities are tested through various applications as well as their safety. Powdered corn silk (1g) was heated in 100 ml of distilled water at 60°C for 1h, cooled, and centrifuged at 4000 rpm for 30 minutes. Then, 1ml of the extract was added to 40ml of 1mM AgNO3 to reduce Ag+ to nano size. Characterization of CS-AgNPs was through UV-visible spectroscopy, Fourier-transform-infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Energy Dispersive X-ray (EDX) analysis. The efficacy of CS-AgNPs as a larvicidal, anticoagulant, thrombolytic, and antioxidant agent was evaluated. Safety evaluation was carried out using Allium ...

Resistance to artemisinins has led to possible anti malarial combination therapy. This study was ... more Resistance to artemisinins has led to possible anti malarial combination therapy. This study was undertaken to investigate the effects of co - administration of artesunate and Azadirachta indica leaves extract on antioxidants and certain liver parameters in rats. 24 male rats weighing averagely 190g were randomly selected into four groups, administered therapeutic doses of artesunate and ext ract namely : Group A (Control), Group B (Extract only)), Group C (Artesunate only) and Group D (Artesunate with extract). Total protein, Cholesterol concentrations, Alanine amino transferase (ALT), Alkaline phosphatase (ALP) and Gamma glutamyl transferase (GGT) activities were determined using international standardized methods. Malondialdehyde, (MDA), reduced glutathione (GSH), phenols, flavonoids, hydroxyl (OH · ) and 1 - 1 - diphenyl - 2 - picryl hydrazyl (DPPH) radicals scavenging effects were determined spectr ophotometrically. Results obtained showed significant increases (P ≤0.05) in pla...

Evidence-Based Complementary and Alternative Medicine, 2020

IOP Conference Series: Materials Science and Engineering, 2020

Animal waste materials are rarely used in the synthesis of nanoparticles compared to microorganis... more Animal waste materials are rarely used in the synthesis of nanoparticles compared to microorganisms and plant materials. The use of animal fur (goat) in synthesis could assist in turning waste to wealth. Thus, potentials of animal fur in the synthesis of silver nanoparticles (AF-AgNPs), its biological activities and safety through cytogenotoxicity were investigated. Animal fur (1 g) was hydrolyzed with 100 ml of 0.1 M NaOH at 90 °C for 1 h, cooled and centrifuged at 4000 rpm for 30 min. The extract (1 ml) was added to 1 mM AgNO3 (40 ml) to reduce Ag+ to its nanoparticles. The AF-AgNPs was characterized using UV–vis spectroscopy, Fourier-transform-infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Energy Dispersive X-ray (EDX) analysis. Larvicidal, antioxidant, anticoagulant and thrombolytic potentials of AF-AgNPs were studied. Onion bulbs (20) were exposed to 0.01, 0.10, 1.0, 10.0 and 100.0 μg/ml of AF-AgNPs solution for its cytogenotoxicity study with AgNO3 so...

IOP Conference Series: Materials Science and Engineering, 2020

Research work on corrosion behaviour of metals and their alloys continues to attract considerable... more Research work on corrosion behaviour of metals and their alloys continues to attract considerable attention because of the huge cost required to prevent corrosion and replace failed or contaminated components due to corrosion reactions. Different inhibitors have been used to minimize corrosion rate, but addition of nanoparticles has been reported to enhance inhibition efficiency. In this work, the influence of silver nanoparticles (AgNPs) on inhibition of corrosion of mild steel, stainless steel and aluminum in 1.0 M HCl were investigated. Biosynthesized AgNPs were acquired from Laboratory of Industrial Nanobiotechnology, Ladoke Akintola University of Technology Ogbomoso. The particles were hitherto characterized using FTIR, UV-Vis and TEM. Five concentrations of AgNPs solution (0, 5, 10, 15, and 20 μg/ml) were added to 1.0 M HCl. The corrosion inhibition effects of the AgNPs on the metal samples were analyzed using gravimetric (weight loss), potentiodynamic polarization and gasomet...

Nanotechnology for Environmental Engineering, 2019

While several green biomolecules have been used to synthesize functional and biologically compati... more While several green biomolecules have been used to synthesize functional and biologically compatible nanoparticles, little attention has been paid to Persea americana (PA) (avocado) fruit extract as a potential reducing agent. This study used avocado fruit peel aqueous extracts to synthesize silver nanoparticles (PAAgNPs), gold nanoparticles (PAAuNPs) and bimetallic alloy nanoparticles (PAAg-AuNPs). The particles were characterized using UV-vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy, selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy and X-ray diffraction among others. The particles were assessed for their antibacterial, antifungal and antioxidant properties. The UV-vis spectroscopy showed PAgNPs, PAAuNPs and PAAg-AuNPs with surface plasmon resonance at 455.5, 538 and 540.5 nm, respectively. The significant FTIR peaks: PAAgNPs (3358 cm −1), PAAuNPs (3503 cm −1) and PAAg-AuNPs (3651 cm −1) pointed to protein as both capping and stabilizing agents for the synthesized nanoparticles. Generally, the particles were spherical, with size range of 18-80 nm for PAAgNPs, 16-71 nm for PAAuNPs and 44-55 nm for PAAg-AuNPs. The energy-dispersive X-ray spectra showed silver, gold and silver/gold as conspicuous metals in PAAgNPs, PAAuNPs and PAAg-AuNPs colloids, respectively. SAED showed ring-shaped patterns for the particles. The nanoparticles effectively inhibited growth of tested bacteria (11-94%) for PAAgNPs, (10-77%) for PAAuNPs and (20-85%) for PAAg-AuNPs. The effectiveness of the biosynthesized nanoparticles can be placed as PAAg-AuNPs > PAAgNPs > PAAuNPs. The fungal inhibition performances are 33-76%, 50-82% and 27-88% for PAAgNPs, PAAuNPs and PAAg-AuNPs, respectively, while DPPH-scavenging activities were 57.82-63.25%, 15.28-54.50% and 53.05-54.26%, which were dose dependent at the tested concentrations of 20-100 µg/ml with good antioxidant activities compared to standard BHA (41.46-84.57%) and ascorbic acid (43.56-91.10%). The bleaching inhibition assay of ABTS showed activities of 56.15-85.43% (PAAgNPs), 34.67-50.93% (PAAuNPs) and 45.31-94.01% (PAAg-AuNPs). The lower concentrations of EC 50 were obtained in nanoparticles (24.45-58.33 µg/ml) compared with the standards (38.42-69.04 µg/ml), indicating that the nanoparticles could suffice as good agents in drug consignment. This study has demonstrated the potential of P. americana fruit peel aqueous extracts to synthesize AgNPs, AuNPs and Ag-AuNPs for antimicrobial and antioxidant applications. The current work, to the best of our knowledge, is the first to use avocado peel extract to synthesis nanoparticles.

Asian Pacific Journal of Health Sciences, 2016

Gentamicin have been shown to generate free radicals .This study investigated modulatory potentia... more Gentamicin have been shown to generate free radicals .This study investigated modulatory potential of aqueous extract of Hibiscus Sabdariffa calyx with gentamicin in rats. Thirty rats weighing between 150-200g were randomly selected into 6 groups: Group A( control), group B received 200mg/kg body weight of extract, group C received 80mg/ kg body weight of gentamicin intra peritoneally for six days while group D, E and F received 200mg/kg, 400mg/kg and 600mg/kg body weight of extract for six days before and eight days following administration of 80mg/kg gentamicin. Antioxidants and biochemical indices were determined using standardized methods . Rats treated with extracts at 200mg/kg elicit significant (p<0.05) decreases in total bilirubin (TB), conjugated bilirubin (C.BIL), urea, creatinine, Malondialdehyde (MDA) concentrations, Aspartate aminotransferase (AST), Alanine aminotansferase (ALT) and Alkaline phosphatase (ALP) activities as well as significant( p<0.05) increases in Superoxide Dismutate (SOD) activity , levels of reduced glutathione (GSH) ,albumin and total protein (TP). Gentamicin treated rats however showed significant (p<0.05) decreases in GSH, TP, albumin and SOD activities with elevated levels of MDA, TB,C.BIL, urea, creatinine and AST, ALT and ALP activities . Furthermore , animals treated with various concentrations of extract after gentamicin administration showed significant increases (p<0.05) in concentrations of TP, ALB, GSH and SOD activities with corresponding decreases in TB, C.BIL, urea, creatinine and MDA concentrations as well as AST,ALT and ALP activities compared with gentamicin group in a dose dependent manner. Properties exhibited by extract is antioxidative suggestive of its modulatory effects on gentamicin activity .

Advances in Life Science and Technology, 2019

Arsenic is an environmental pollutant and its contamination in the drinking water is considered a... more Arsenic is an environmental pollutant and its contamination in the drinking water is considered as a serious worldwide environmental health threat. The present study investigated the effects of arsenic exposure on antioxidant parameters and p53 expression in male albino rats. The animals (n=45) were exposed to arsenic (100 ppm, 150 ppm and 200 ppm) for 4, 8 and 12 weeks as sodium arsenate in drinking water. Control animals (n=15) received distilled water for the same period. Hepatic superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), reduced glutathione (GSH), lipid peroxidation and total protein were evaluated spectrophotometrically. Expression of p53 was also detected by histochemical staining. Before the commencement of arsenic exposure, five animals were sacrificed to obtain baseline data. ANOVA followed by Tukey's test was used to analyse the results with p<0.05 considered significant. Significant decrement in hepatic activities of SOD, catalase and GPx as well as hepatic concentration of GSH and total protein concentration characterized exposure to all the dose regimens of inorganic arsenic at all the time interval. Corroboratively, significant elevation was observed in malondialdehyde (MDA). The expression of p53 decreased in the groups that were exposed to arsenic as compared to the control animals. The findings from the present study suggests excessive generation of free radicals and reduction in p53 expression in arsenic-induced hepatotoxicity.

Aluminium phosphide is an oral rodenticide and insecticide that is toxic to multiple organs in no... more Aluminium phosphide is an oral rodenticide and insecticide that is toxic to multiple organs in non-target organisms with no known antidote. This study investigated aluminium phosphide-induced hematotoxicity and ovarian oxidative damage in rat and evaluated the effectiveness of hesperidin as a therapeutic agent against the toxic effects. Rats receiving a sub-lethal dose of aluminium phosphide (1.15 mg kg-1 body weight) for 30 days, exhibited significant impaired hematological parameters with distorted leucocyte and thrombocytic indices. Aluminium phosphide exposure produced macrocytic, hyperchromic anemia. The toxicity also included a reduction in the white blood cell, lymphocyte, and granulocyte counts. Ovarian oxidative stress indicators comprising malonaldehyde and lipid hydroperoxides levels were markedly increased, while the antioxidant enzymatic system was inhibited. Co-treatment with hesperidin ameliorated the aluminum phosphide-induced anemia, thrombocytopenia and leukopenia ...

Journal of Pharmacology and Toxicology, 2012

Nano Plus: Science and Technology of Nanomaterials, 2021

The Nanotechnology Research Group (NANO+) at Ladoke Akintola University of Technology, Ogbomoso, ... more The Nanotechnology Research Group (NANO+) at Ladoke Akintola University of Technology, Ogbomoso, Nigeria organized her 5th international conference on nanotechnology on 26-28 October 2021 using both physical and Zoom platforms (hybrid). The conference coded ‘LAUTECH NANO 2021’ with the theme ‘Nanotechnology in Africa: Changing the Narratives for the Benefits of Mankind’ provided a platform for African nations to explore nanotechnology to engender the much-desired growth and development on the continent, and to also bridge the gap of nanotechnology divide between Africa and the developed nations. In this regard, active players and seasoned scholars in nanotechnology R&D in Africa and other countries shared their experiences and visions with participants. In addition, fifty-seven (57) papers were presented at the technical sessions in diverse areas of science, engineering, agriculture, and biomedicine from 45 institutions/agencies in more than 10 countries. This book of abstracts cont...

BMC Complementary and Alternative Medicine, 2019

Background: The plant Holarrhena floribunda (H. floribunda; G. Don) is indigenous to sub-Saharan ... more Background: The plant Holarrhena floribunda (H. floribunda; G. Don) is indigenous to sub-Saharan Africa and is traditionally used to treat several ailments. The present study was carried out to isolate and characterize bioactive compounds with anti-proliferative activity present in H. floribunda extracts. Methods: Compounds were isolated from H. floribunda using the bioassay-guided fractionation technique of repeated column chromatography and the step-wise application of the MTT reduction assay to assess antiproliferative bioactivity. The structures of the compounds were identified mainly using NMR. The effects of the isolated compounds on the viability, cell cycle and proliferation of human cancer cell lines (MCF-7, HeLa and HT-29) as well as the non-cancerous human fibroblast cell line (KMST-6) were investigated. Results: Bioassay-guided fractionation yielded two steroidal alkaloids: holamine (1) and funtumine (2). The MTT reduction assay shows that both compounds exhibited selective dose-dependent cytotoxicity against the cancer cell lines studied. The isolated compounds induced cell cycle arrest at the G 0 /G 1 and G 2 /M phases in the cancer cell lines with significant reduction in DNA synthesis. The results obtained show that the cancer cells (MCF-7, HeLa and HT-29) used in this study were more sensitive to the isolated compounds compared to the noncancerous fibroblast cells (KMST-6). Conclusion: The ability of the isolated compounds to cause cell cycle arrest and reduce DNA synthesis raises hopes for their possible development and use as potent anticancer drugs. However, more mechanistic studies need to be done for complete validation of the efficacy of the two compounds.

Journal of Natural Sciences Research, 2019

Arsenic exposure has been implicated by several epidemiological studies as an important metalloid... more Arsenic exposure has been implicated by several epidemiological studies as an important metalloid that is currently poisoning millions of people globally. In order to investigate the time-course of arsenic exposure on hepatic and renal toxicity, male albino rats (n=45) were exposed to arsenic (100 ppm, 150 ppm and 200 ppm) for 4, 8 and 12 weeks as sodium arsenate in their drinking water. Control animals (n=15) received distilled water for the same period after which blood and vital organs were removed from the animals and analyzed for alanine amino transaminase (ALT), aspartate amino transaminase (AST) gamma amino transaminase (γGT), alkaline phosphatase (ALP), creatinine and urea spectrophotometrically. Histological changes in hepatocytes was also examined. Before the commencement of arsenic exposure, five animals were sacrificed to obtain baseline data. Significant elevation in plasma ALT, AST, γGT and alkaline phosphatase activities characterized the effect of the arsenical at all doses and time interval relative to the controls. Plasma levels of creatinine and urea were also elevated at all-time intervals in the arsenic group. In most of the cases observed, the elevated level of these biochemical marker in circulation are time-and dose-dose dependent. Hepatic histopathology reveals degeneration of cytoplasmic contents, evidence of necrosis, collapse of central vein, cytoplasmic inclusion and enlarged hepatic sinusoids in arsenic-exposed groups. These findings suggest that different dose regimens of sodium arsenate at different time interval caused degenerative changes in hepatic and renal tissues in rats in dose-and time-dependent fashion.

Antineoplastic drugs are well known for their abilities to induce varying degrees of toxicities a... more Antineoplastic drugs are well known for their abilities to induce varying degrees of toxicities and predispose to cardiovascular related disease states. This study, therefore evaluates the effects of Doxorubicin (Dox) (3 mg/kg body weight) and Etoposide (Etop) (3 mg/kg body weight) alone and in combinations at 12 and 24 h intervals on plasma lipid profiles and paraoxonase-1. Lipid peroxidation, superoxide dismutase (SOD), glutathione peroxidase (Gpx) and reduced glutathione (GSH) in selected male rat organs were also evaluated. The results were analyzed using One-way ANOVA followed by post hoc tests. Hypocholesterolemia characterized the effects of Etop alone and in combination with Dox at 12 h, while at 24 h interval hypercholesterolemia was observed in the combined group. Both drugs induced significant hypertriglyc-eridemia except at 12 h combination with significant hypotriglyceridemia. Individually , the drugs significantly reduced HDL-cholesterol (HDL-C) contrarily to up-regula...

Journal of Hazardous Materials Advances, 2022

Arsenic is a naturally occurring and ubiquitously present metalloid in soil, water, food, and t... more Arsenic is a naturally occurring and ubiquitously present metalloid in soil, water, food,
and the environment. Arsenic exposure to human through various sources such as contaminated groundwater and other human activities have become a significant global concern. This is because arsenic has been shown to exhibit extreme toxic
potential with serious health implications. Its exposure to humans is linked to myriads of disorders and this represents a major threat to the health, economic, and social well-being of people, especially, in the less-developed countries of the world. Major diseases that have been linked to arsenic poisoning are diabetes, hyperkeratosis,
cancer, hypertension, and neurodegeneration, etc. Meanwhile, to date, there is no specific or approved remedy for arsenic poisoning. Chelation therapy for the management of arsenic poisoning is fraught with several side effects which limit its clinical usefulness. In this review article, we tried to provide an insight into some
of the key scientific details that are documented in the literature as regards arsenic toxicity. These include its physicochemical properties, means of exposure, and possible mechanism of arsenic-induced toxicity. Furthermore, we also beamed our searchlight on the effect of arsenic on some major organs of the body. Online search
Engines such as Google®, Scholar google®, SciFinder®, and PubMed® were used for literature search. Scientific journals that relate to the objective of this review without restriction to the year of publication were considered. This study revealed the significance of arsenic toxicity and its contribution to health-related challenges.
Therefore, it is pertinent for policy Makers to increase awareness of arsenic toxicity and formulate guidelines for a cleaner environment. This will significantly reduce the incidence of arsenic-induced non-communicable
diseases

Oxidative damage is a factor in human diseases. In this study, effects of extract and fractions o... more Oxidative damage is a factor in human diseases. In this study, effects of extract and fractions of Solanium aethiopicum leaves on certain liver function indices, anti oxidative and anti-lipid peroxidation potentials were investigated. Phenols, flavonoids, antioxidative potentials, enzyme assays were determined spectrophotometrically using standardized methods. Results showed rats fed methanol extract (ME) at 50, 100, 150 and 200 mg/kg body weight for 21 days showed significant (P<0.05) decreases in liver Malondialdehyde and cholesterol concentrations by 2.530±0.050, 1.780±0.020, 1.670±0.030, 1.480±0.040 mg/ml protein and 69.290±2.660, 46.980±2.500, 39.030±4.820, 34.650±1.660 mg/dl respectively. At these concentrations, ME exhibited significant (p<0.05) decreases in serum Gamma glutamyl transferase (GGT) activity by 3.810 ± 0.010, 2.380 ± 0.010, 2.020 ± 0.010 and 1.190±0.010 IU/L compared with controls (3.830 ± 0.020 IU/L). Phenols and flavonoids of ME, Ethyl acetate fraction (...

Acta Biochimica Polonica

Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxyg... more Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxygen species (ROS). ROS oxidize cellular fatty acids to produce lipid peroxyl radicals, subsequently transformed into lipid peroxides, which decrease membrane fluidity and increase the activity of various enzymes implicated in degenerative diseases and cancer formation. Edible plants that contain exogenous compounds like curcumeroid, β-carotene, turmeric, and so on, protect the aerobic cells from oxidation of free radicals. This study thus evaluates antioxidant and antimutagenic activities of ethyl acetate, aqueous and methanolic fractions of Holarrhena floribunda leaves. Inhibitory activities of the ethyl acetate fraction on Fe(2+)-induced lipid peroxidation in hen egg yolk; rat liver and brain tissues were also evaluated. The Allium cepa root assay was used to evaluate antimutagenic activity. Results showed that the ethyl acetate scavenged DPPH, OH•, and •O2(-) much stronger than other f...