Emel Özkan Ünal | Tekirdağ Namık Kemal üniversitesi (original) (raw)
Papers by Emel Özkan Ünal
Archives Animal Breeding
Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the syn... more Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the synthesis rate triglyceride from diacylglycerol. Leptin (LP, OB, obese) is an important hormone that synthesizes mostly from adipose tissue and regulates glucose metabolism and homeostasis. DGAT1 and Lep genes are closely related to reproduction, growth, milk yield and composition in water buffalo breeds. This study aimed to identify genetic variation in the DGAT1 and Lep gene regions in 150 water buffalo individuals from five different provinces of Turkey using DNA sequencing. A total of 38 nucleotide variations and indels have identified 761 bp long partial intron 2 and exon 3 and 5 UTR regions of the Lep gene in Anatolian water buffalo populations; 422 bp long partial exon 7-9 and exon 8 regions of DGAT1 gene were amplified and two mutations were defined in the point of 155 and 275 nucleotide that is three genotypes for S allele and Y allele of DGAT1 gene in intron 7 in Anatolian buffalo populations, respectively. These SNPs may have an effect on reproduction, growth, milk yield and composition in water buffalo populations and may prove to be useful for water buffalo breeding.
Turkish Journal of Veterinary & Animal Sciences
Introduction According to the data by the Turkish Statistical Institute (TURKSTAT), the number of... more Introduction According to the data by the Turkish Statistical Institute (TURKSTAT), the number of donkeys in Turkey was 943,751 head in 1991 but decreased to 126,912 head in recently and the rate of decrease in the number of donkeys in Turkey was calculated as 87% [1]. The donkey stock on earth was about 37 million head 1961, while this number was reported as about 50.5 million head in recently by the FAO. The number of donkeys has rapidly decreased in such countries as Bulgaria, China, France, Italy, Greece, and Turkey [2]. There remained no need for donkeys in the rural section upon development, which caused both a decrease in the number of donkeys and the extinction of donkey breeds. The fact that the composition of donkey's milk is close to that of human's milk, [3-5]. It was seen that whilst the donkey stock was increasing slowly on earth, the number of donkeys decreased and some donkey breeds were endangered in both Europe and our country. There were 140 donkey breeds in the category of native breeds in the world and that the most breeds were in Asia, Europe, and the Caucasus [6]. The ancestors of the domestic donkey (Equus asinus asinus) are reported to have inhabited the deserts in the Sudan, Ethiopia, and Somali. Characteristically, the Nubian ass has a dorsal stripe on the backbone, whereas the Somali ass lacks it. While the feet are white and the crosswise stripe on the shoulder draws attention in the Nubian ass, the Somali ass is light red, lacks a crosswise
Hayvan Bilimi ve Ürünleri Dergisi, Dec 27, 2020
Hayvan Bilimi ve Ürünleri Dergisi, Dec 24, 2018
In this study, 3843 lactation yield records of Anatolian Buffaloes within "Anatolian Water Buffal... more In this study, 3843 lactation yield records of Anatolian Buffaloes within "Anatolian Water Buffalo Breeding Project" and reared in Istanbul province and district were used. The aim of study was to investigate the effects of environmental factors on the lactation milk yield (LMY), lactation length (LL) and calving interval (CI) of the Anatolian buffalo. For this propose 2034 Anatolian buffalos' pedigrees in Istanbul between 2012 to 2017 were used. The overall mean and standard error of the LMY, LL and CI were determined as 1223.9 ± 6.83 kg, 230.99 ± 0.89 kg and 417.51 ± 1.73 days respectively. The effects of the province, calving year, lactation number, season and calving age on these characteristics were determined. Also effects of the province, calving year, lactation number, season and calving age on LMY, effect of province, calving year and season on LL and calving year, lactation number and calving age on CI were statistically significant (p<0.01), Phenotypic correlation were calculated between LMY, LL and CI also.
This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorp... more This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorphisms in 150 water buffalo ( Bubalus bubalis ) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3' UTR regions of the GH gene (also called GH/Alu I locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/Msp I locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/Msp I locus. A missense mutation from glycine to glutamate amino acid and four silent mutations in the serine, threonine, and asparagine amino acids were determined in the exon 3 region of the GH gene. Four genotypes due to eight SNP were identified in a 404 bp long region of the GH/Alu I locus. A missense mutation from lysine to arginine amino acid and six silent mutations in Leucine, aspartat...
Frontiers in genetics, 2018
The domestic water buffalo is native to the Asian continent but through historical migrations and... more The domestic water buffalo is native to the Asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. The two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. River buffaloes from Pakistan, Iran, Turkey, Egypt, Romania, Bulgaria, Italy, Mozambique, Brazil and Colombia, and swamp buffaloes from China, Thailand, Philippines, Indonesia and Brazil were genotyped with a species-specific medium-density 90K SNP panel. We estimated the levels of molecular diversity and described population structure, which revealed historical relationships between populations and migration events. Three distinct gene pools were identified in pure river as well as in pure swamp buffalo populations. Genomic admixture was seen in the Philippines and in Brazil, resulting from importations of animals for breed improvement...
vethekimder.org.tr
Abstract: This study was supported by TUBITAK TURKHAYGEN-I (In Vitro Conservation and Preliminary... more Abstract: This study was supported by TUBITAK TURKHAYGEN-I (In Vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I) project. In order to investigate the genetic diversity of goat has been carried out with 20 ...
The domestic water buffalo is native to the Asian continent but through historical migrations and... more The domestic water buffalo is native to the Asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. The two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. River buffaloes from Pakistan, Iran, Turkey, Egypt, Romania, Bulgaria, Italy, Mozambique, Brazil and Colombia, and swamp buffaloes from China, Thailand, Philippines, Indonesia and Brazil were genotyped with a species-specific medium-density 90K SNP panel. We estimated the levels of molecular diversity and described population structure, which revealed historical relationships between populations and migration events. Three distinct gene pools were identified in pure river as well as in pure swamp buffalo populations. Genomic admixture was seen in the Philippines and in Brazil, resulting from importations of animals for breed improvement. Our results were largely consistent with previous archeological, historical and molecular-based evidence for two independent domestication events for river- and swamp-type buffaloes, which occurred in the Indo-Pakistani region and close to the China/Indochina border, respectively. Based on a geographical analysis of the distribution of diversity, our evidence also indicated that the water buffalo spread out of the domestication centers followed two major divergent migration directions: river buffaloes migrated west from the Indian sub-continent while swamp buffaloes migrated from northern Indochina via an east-south-eastern route. These data suggest that the current distribution of water buffalo diversity has been shaped by the combined effects of multiple migration events occurred at different stages of the post-domestication history of the species
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 2021
Introduction The total number of goats in the world is estimated at approximately one billion hea... more Introduction The total number of goats in the world is estimated at approximately one billion heads of which ten million are raised in Turkey, which corresponds almost half of the entire Europe [1]. In Turkey, while goat breeding is mainly based on Hair goats (98%) reared for dual purpose (meat and milk yield), Angora goats estimated at 210.000 heads (2%) are raised for mohair production. In 2018, 6000 tons of hair and 370 tons of mohair were produced from Hair and Angora goats, respectively [2]. The countries that produce mohair are South Africa, the USA,
Turkish Journal of Veterinary and Animal Sciences, 2021
The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by ... more The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by genetic variation of keratin associated protein genes as KAP 1.1 (Keratin Associated Protein 1.1, formerly known as B2A), KAP1.3 (Keratin Associated Protein 1.3, formerly known as B2C) and K33 (Keratin Intermediate Filaments Type I, formerly known as KRT1.2). Keratin-associated proteins play a significant role in identifying structural and mechanical properties of the hair and wool fibres. This study was conducted to detect genetic variation at the KAP1.1, KAP1.3 and K33 genes in indigenous Turkish goat populations using DNA sequencing method. The DNA of 100 individuals selected from 5 different native goat breeds (Hair, Honamlı, Kilis, Norduz, and Angora) that reared different regions of Turkey were used as materials. A total of 59 nucleotide variations and indels (insertion/deletion) of KAP1.1 gene, 15 nucleotide variations and indels of KAP1.3 gene, 16 nucleotide variations of K33 gene were determined in the studied samples. These nucleotide variations and indels have been causing changes in the number and sequence of amino acids. It is necessary to determine the relationships with mohair yield, quality and polymorphisms that are determined in KAP1.1, KAP1.3 and KRT1.2 genes.
Animals, 2021
The present study has been one of the first attempts to thoroughly examine the effects of differe... more The present study has been one of the first attempts to thoroughly examine the effects of different kefir sources on fermentation characteristics, aerobic stability, and microbial communities of alfalfa silages. The effects of commercial kefir (CK) and homemade kefir culture (HK) applied with untreated a common control (CON) and three different application doses (5.0, 5.7, and 6.0 log cfu g1) on wilted alfalfa and stored at an ambient temperature of 25–30 C are studied. After 45 days of ensiling, fermentation characteristics and aerobic stability of silages were measured, and bacterial diversity was investigated by 16S ribosomal RNA gene sequencing using the GenomeLab™ GeXP platform. Both CK and HK accelerate more lactic acid production and reduced ammonia nitrogen concentration. Factor analysis of kefir sources suggests that the addition of kefir improves the aerobic stability of silages, even the initial water-soluble carbohydrate (WSC) content is inadequate via its antimicrobial effect on yeast and mold formation. Enterococcus faecium, Pediococcus pentosaceous and Lactobacillus brevis were dominant bacterial species among the treated groups at silo opening, while Lactobacillus plantarum and Lactobacillus brevis became dominant bacterial species after 7 days of aerobic exposure. In conclusion, the application of kefir on alfalfa silages improves fermentation quality and aerobic stability even with low WSC content.
Arch. Anim. Breed, 2022
Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the syn... more Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the synthesis rate triglyceride from diacylglycerol. Leptin (LP, OB, obese) is an important hormone that synthesizes mostly from adipose tissue and regulates glucose metabolism and homeostasis. DGAT1 and Lep genes are closely related to reproduction, growth, milk yield and composition in water buffalo breeds. This study aimed to identify genetic variation in the DGAT1 and Lep gene regions in 150 water buffalo individuals from five different provinces of Turkey using DNA sequencing. A total of 38 nucleotide variations and indels have identified 761 bp long partial intron 2 and exon 3 and 5 UTR regions of the Lep gene in Anatolian water buffalo populations; 422 bp long partial exon 7-9 and exon 8 regions of DGAT1 gene were amplified and two mutations were defined in the point of 155 and 275 nucleotide that is three genotypes for S allele and Y allele of DGAT1 gene in intron 7 in Anatolian buffalo populations, respectively. These SNPs may have an effect on reproduction, growth, milk yield and composition in water buffalo populations and may prove to be useful for water buffalo breeding.
Brazilian Journal of Animal Science, 2020
This study was conducted to investigate the growth hormone (GH; somatotropin-like) gene polymorph... more This study was conducted to investigate the growth hormone (GH; somatotropin-like) gene polymorphisms in 150 water buffalo (Bubalus bubalis) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3' UTR regions of the GH gene (also called GH/AluI locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/MspI locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/MspI locus. A missense mutation from glycine to glutamate amino acid and four silent mutations in the serine, threonine, and asparagine amino acids were determined in the exon 3 region of the GH gene. Four genotypes due to eight SNP were identified in a 404 bp long region of the GH/AluI locus. A missense mutation from lysine to arginine amino acid and six silent mutations in Leucine, aspartate, histidine, lysine, arginine, and cysteine amino acids were revealed in the exon 5 region of the GH gene. The partial DNA sequence of the GH gene in water buffalos was reported, and these sequences were deposited at the NCBI Genbank database with the accession numbers MN266903-MN266909 and MN530973-MN530976. These SNP may have an effect on economic (such as body composition) and carcass traits, reproduction, and milk yield and content in water buffalo populations and may prove to be useful for water buffalo breeding.
Animals
The present study has been one of the first attempts to thoroughly examine the effects of differe... more The present study has been one of the first attempts to thoroughly examine the effects of different kefir sources on fermentation characteristics, aerobic stability, and microbial communities of alfalfa silages. The effects of commercial kefir (CK) and homemade kefir culture (HK) applied with untreated a common control (CON) and three different application doses (5.0, 5.7, and 6.0 log cfu g−1) on wilted alfalfa and stored at an ambient temperature of 25–30 °C are studied. After 45 days of ensiling, fermentation characteristics and aerobic stability of silages were measured, and bacterial diversity was investigated by 16S ribosomal RNA gene sequencing using the GenomeLab™ GeXP platform. Both CK and HK accelerate more lactic acid production and reduced ammonia nitrogen concentration. Factor analysis of kefir sources suggests that the addition of kefir improves the aerobic stability of silages, even the initial water-soluble carbohydrate (WSC) content is inadequate via its antimicrobia...
Revue De Medecine Veterinaire, 2005
SUMMARY The Turkish native sheep breeds, possibly being the neighbours of the earliest domesticat... more SUMMARY The Turkish native sheep breeds, possibly being the neighbours of the earliest domesticated sheep populations, might be harbouring important genetic characteristics to be employed in the future for the improvement of sheep breeds. In order to design a conservation strategy, their genetic diversities must be determined. In the present study, based on three microsatellite loci, the genetic diversity of the Kivircik, Awassi, Akkaraman breeds (native) of Turkey as well as two of their crossbreeds Turkgeldi and Konya Merino were studied comparatively. It was observed that their heterozygoties are all high (0.6673-0.7822) compared to previously studied breeds, as expected for populations close to the center of domestication. Neighbour Joining (NJ) tree based on allele sharing distances indicated that the inertia of the breeds are not high. Yet, the genetic differentiations between the breeds based on pairwise FST (inbreeding coefficient) values are all significant. Furthermore, th...
In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Kara... more In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Karagül breeds of Anatolia, wild sheep Anatolian Mouflon (Ovis gmelinii anatolica) were used as the study material. Based on SRY and SRYM18 regions on the Y-chromosome, haplotypes of the populations were analyzed using DNA sequence analyses. The SRY region, A-oY1 allele was observed in all of the individuals studied. On the other hand, four different alleles corresponding to four Y-chromosome haplotypes were detected at the SRYM18 microsatellite region. Among native Anatolian breeds (n=143), H6 haplotype (80.41%), H4 haplotype (9.09%), H8 haplotype (8.40%) and H12 haplotype (2.1%) were identified. H6 haplotype was observed in all 16 individuals of Ovis gmelinii anatolica. Pairwise FST values based on haplotype frequencies were calculated for domestic sheep, and the highest FST value was observed between Karagül and Kıvırcık along with Karagül and Ovis gmelinii anatolica with pairwise FST val...
Revista Brasileira de Zootecnia
Animals : an Open Access Journal from MDPI, 2021
Simple Summary In the present study, twenty microsatellite loci were tested to assess and analyze... more Simple Summary In the present study, twenty microsatellite loci were tested to assess and analyze the genetic diversity between and within 17 different populations of Turkish water buffalo. The total number of animals sampled was 837, collected from six geographical regions: Marmara Region (MRM), Black Sea Region (BSR), Aegean Region (AER), Central Anatolia Region (CAR), Eastern Anatolia Region (EAR) and Southeastern Anatolia Region (SAR). All studied microsatellites markers showed allelic polymorphism. In this study, the results indicated a definite genetic diversity among the Turkish water buffalo populations which indicates the existence of at least two major clusters. Abstract The present study was aimed to investigate the genetic diversity among 17 Turkish water buffalo populations. A total of 837 individuals from 17 provincial populations were genotyped, using 20 microsatellites markers. The microsatellite markers analyzed were highly polymorphic with a mean number of alleles ...
Archives Animal Breeding
Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the syn... more Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the synthesis rate triglyceride from diacylglycerol. Leptin (LP, OB, obese) is an important hormone that synthesizes mostly from adipose tissue and regulates glucose metabolism and homeostasis. DGAT1 and Lep genes are closely related to reproduction, growth, milk yield and composition in water buffalo breeds. This study aimed to identify genetic variation in the DGAT1 and Lep gene regions in 150 water buffalo individuals from five different provinces of Turkey using DNA sequencing. A total of 38 nucleotide variations and indels have identified 761 bp long partial intron 2 and exon 3 and 5 UTR regions of the Lep gene in Anatolian water buffalo populations; 422 bp long partial exon 7-9 and exon 8 regions of DGAT1 gene were amplified and two mutations were defined in the point of 155 and 275 nucleotide that is three genotypes for S allele and Y allele of DGAT1 gene in intron 7 in Anatolian buffalo populations, respectively. These SNPs may have an effect on reproduction, growth, milk yield and composition in water buffalo populations and may prove to be useful for water buffalo breeding.
Turkish Journal of Veterinary & Animal Sciences
Introduction According to the data by the Turkish Statistical Institute (TURKSTAT), the number of... more Introduction According to the data by the Turkish Statistical Institute (TURKSTAT), the number of donkeys in Turkey was 943,751 head in 1991 but decreased to 126,912 head in recently and the rate of decrease in the number of donkeys in Turkey was calculated as 87% [1]. The donkey stock on earth was about 37 million head 1961, while this number was reported as about 50.5 million head in recently by the FAO. The number of donkeys has rapidly decreased in such countries as Bulgaria, China, France, Italy, Greece, and Turkey [2]. There remained no need for donkeys in the rural section upon development, which caused both a decrease in the number of donkeys and the extinction of donkey breeds. The fact that the composition of donkey's milk is close to that of human's milk, [3-5]. It was seen that whilst the donkey stock was increasing slowly on earth, the number of donkeys decreased and some donkey breeds were endangered in both Europe and our country. There were 140 donkey breeds in the category of native breeds in the world and that the most breeds were in Asia, Europe, and the Caucasus [6]. The ancestors of the domestic donkey (Equus asinus asinus) are reported to have inhabited the deserts in the Sudan, Ethiopia, and Somali. Characteristically, the Nubian ass has a dorsal stripe on the backbone, whereas the Somali ass lacks it. While the feet are white and the crosswise stripe on the shoulder draws attention in the Nubian ass, the Somali ass is light red, lacks a crosswise
Hayvan Bilimi ve Ürünleri Dergisi, Dec 27, 2020
Hayvan Bilimi ve Ürünleri Dergisi, Dec 24, 2018
In this study, 3843 lactation yield records of Anatolian Buffaloes within "Anatolian Water Buffal... more In this study, 3843 lactation yield records of Anatolian Buffaloes within "Anatolian Water Buffalo Breeding Project" and reared in Istanbul province and district were used. The aim of study was to investigate the effects of environmental factors on the lactation milk yield (LMY), lactation length (LL) and calving interval (CI) of the Anatolian buffalo. For this propose 2034 Anatolian buffalos' pedigrees in Istanbul between 2012 to 2017 were used. The overall mean and standard error of the LMY, LL and CI were determined as 1223.9 ± 6.83 kg, 230.99 ± 0.89 kg and 417.51 ± 1.73 days respectively. The effects of the province, calving year, lactation number, season and calving age on these characteristics were determined. Also effects of the province, calving year, lactation number, season and calving age on LMY, effect of province, calving year and season on LL and calving year, lactation number and calving age on CI were statistically significant (p<0.01), Phenotypic correlation were calculated between LMY, LL and CI also.
This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorp... more This study was conducted to investigate the growth hormone ( GH; somatotropin-like) gene polymorphisms in 150 water buffalo ( Bubalus bubalis ) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3' UTR regions of the GH gene (also called GH/Alu I locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/Msp I locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/Msp I locus. A missense mutation from glycine to glutamate amino acid and four silent mutations in the serine, threonine, and asparagine amino acids were determined in the exon 3 region of the GH gene. Four genotypes due to eight SNP were identified in a 404 bp long region of the GH/Alu I locus. A missense mutation from lysine to arginine amino acid and six silent mutations in Leucine, aspartat...
Frontiers in genetics, 2018
The domestic water buffalo is native to the Asian continent but through historical migrations and... more The domestic water buffalo is native to the Asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. The two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. River buffaloes from Pakistan, Iran, Turkey, Egypt, Romania, Bulgaria, Italy, Mozambique, Brazil and Colombia, and swamp buffaloes from China, Thailand, Philippines, Indonesia and Brazil were genotyped with a species-specific medium-density 90K SNP panel. We estimated the levels of molecular diversity and described population structure, which revealed historical relationships between populations and migration events. Three distinct gene pools were identified in pure river as well as in pure swamp buffalo populations. Genomic admixture was seen in the Philippines and in Brazil, resulting from importations of animals for breed improvement...
vethekimder.org.tr
Abstract: This study was supported by TUBITAK TURKHAYGEN-I (In Vitro Conservation and Preliminary... more Abstract: This study was supported by TUBITAK TURKHAYGEN-I (In Vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I) project. In order to investigate the genetic diversity of goat has been carried out with 20 ...
The domestic water buffalo is native to the Asian continent but through historical migrations and... more The domestic water buffalo is native to the Asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. The two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. River buffaloes from Pakistan, Iran, Turkey, Egypt, Romania, Bulgaria, Italy, Mozambique, Brazil and Colombia, and swamp buffaloes from China, Thailand, Philippines, Indonesia and Brazil were genotyped with a species-specific medium-density 90K SNP panel. We estimated the levels of molecular diversity and described population structure, which revealed historical relationships between populations and migration events. Three distinct gene pools were identified in pure river as well as in pure swamp buffalo populations. Genomic admixture was seen in the Philippines and in Brazil, resulting from importations of animals for breed improvement. Our results were largely consistent with previous archeological, historical and molecular-based evidence for two independent domestication events for river- and swamp-type buffaloes, which occurred in the Indo-Pakistani region and close to the China/Indochina border, respectively. Based on a geographical analysis of the distribution of diversity, our evidence also indicated that the water buffalo spread out of the domestication centers followed two major divergent migration directions: river buffaloes migrated west from the Indian sub-continent while swamp buffaloes migrated from northern Indochina via an east-south-eastern route. These data suggest that the current distribution of water buffalo diversity has been shaped by the combined effects of multiple migration events occurred at different stages of the post-domestication history of the species
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 2021
Introduction The total number of goats in the world is estimated at approximately one billion hea... more Introduction The total number of goats in the world is estimated at approximately one billion heads of which ten million are raised in Turkey, which corresponds almost half of the entire Europe [1]. In Turkey, while goat breeding is mainly based on Hair goats (98%) reared for dual purpose (meat and milk yield), Angora goats estimated at 210.000 heads (2%) are raised for mohair production. In 2018, 6000 tons of hair and 370 tons of mohair were produced from Hair and Angora goats, respectively [2]. The countries that produce mohair are South Africa, the USA,
Turkish Journal of Veterinary and Animal Sciences, 2021
The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by ... more The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by genetic variation of keratin associated protein genes as KAP 1.1 (Keratin Associated Protein 1.1, formerly known as B2A), KAP1.3 (Keratin Associated Protein 1.3, formerly known as B2C) and K33 (Keratin Intermediate Filaments Type I, formerly known as KRT1.2). Keratin-associated proteins play a significant role in identifying structural and mechanical properties of the hair and wool fibres. This study was conducted to detect genetic variation at the KAP1.1, KAP1.3 and K33 genes in indigenous Turkish goat populations using DNA sequencing method. The DNA of 100 individuals selected from 5 different native goat breeds (Hair, Honamlı, Kilis, Norduz, and Angora) that reared different regions of Turkey were used as materials. A total of 59 nucleotide variations and indels (insertion/deletion) of KAP1.1 gene, 15 nucleotide variations and indels of KAP1.3 gene, 16 nucleotide variations of K33 gene were determined in the studied samples. These nucleotide variations and indels have been causing changes in the number and sequence of amino acids. It is necessary to determine the relationships with mohair yield, quality and polymorphisms that are determined in KAP1.1, KAP1.3 and KRT1.2 genes.
Animals, 2021
The present study has been one of the first attempts to thoroughly examine the effects of differe... more The present study has been one of the first attempts to thoroughly examine the effects of different kefir sources on fermentation characteristics, aerobic stability, and microbial communities of alfalfa silages. The effects of commercial kefir (CK) and homemade kefir culture (HK) applied with untreated a common control (CON) and three different application doses (5.0, 5.7, and 6.0 log cfu g1) on wilted alfalfa and stored at an ambient temperature of 25–30 C are studied. After 45 days of ensiling, fermentation characteristics and aerobic stability of silages were measured, and bacterial diversity was investigated by 16S ribosomal RNA gene sequencing using the GenomeLab™ GeXP platform. Both CK and HK accelerate more lactic acid production and reduced ammonia nitrogen concentration. Factor analysis of kefir sources suggests that the addition of kefir improves the aerobic stability of silages, even the initial water-soluble carbohydrate (WSC) content is inadequate via its antimicrobial effect on yeast and mold formation. Enterococcus faecium, Pediococcus pentosaceous and Lactobacillus brevis were dominant bacterial species among the treated groups at silo opening, while Lactobacillus plantarum and Lactobacillus brevis became dominant bacterial species after 7 days of aerobic exposure. In conclusion, the application of kefir on alfalfa silages improves fermentation quality and aerobic stability even with low WSC content.
Arch. Anim. Breed, 2022
Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the syn... more Acyl-CoA: diacylglycerol-acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the synthesis rate triglyceride from diacylglycerol. Leptin (LP, OB, obese) is an important hormone that synthesizes mostly from adipose tissue and regulates glucose metabolism and homeostasis. DGAT1 and Lep genes are closely related to reproduction, growth, milk yield and composition in water buffalo breeds. This study aimed to identify genetic variation in the DGAT1 and Lep gene regions in 150 water buffalo individuals from five different provinces of Turkey using DNA sequencing. A total of 38 nucleotide variations and indels have identified 761 bp long partial intron 2 and exon 3 and 5 UTR regions of the Lep gene in Anatolian water buffalo populations; 422 bp long partial exon 7-9 and exon 8 regions of DGAT1 gene were amplified and two mutations were defined in the point of 155 and 275 nucleotide that is three genotypes for S allele and Y allele of DGAT1 gene in intron 7 in Anatolian buffalo populations, respectively. These SNPs may have an effect on reproduction, growth, milk yield and composition in water buffalo populations and may prove to be useful for water buffalo breeding.
Brazilian Journal of Animal Science, 2020
This study was conducted to investigate the growth hormone (GH; somatotropin-like) gene polymorph... more This study was conducted to investigate the growth hormone (GH; somatotropin-like) gene polymorphisms in 150 water buffalo (Bubalus bubalis) from different regions of Turkey. 404 bp long partial intron 4, exon 5, 3' UTR regions of the GH gene (also called GH/AluI locus) and 347 bp long exon-intron 3 and partial exon 4 regions of the GH gene (also called GH/MspI locus) were amplified, and their PCR products analyzed via DNA sequencing method. Seven genotypes due to twenty single nucleotide polymorphisms (SNP) and one deletion/insertion were identified in a 347 bp long region of the GH/MspI locus. A missense mutation from glycine to glutamate amino acid and four silent mutations in the serine, threonine, and asparagine amino acids were determined in the exon 3 region of the GH gene. Four genotypes due to eight SNP were identified in a 404 bp long region of the GH/AluI locus. A missense mutation from lysine to arginine amino acid and six silent mutations in Leucine, aspartate, histidine, lysine, arginine, and cysteine amino acids were revealed in the exon 5 region of the GH gene. The partial DNA sequence of the GH gene in water buffalos was reported, and these sequences were deposited at the NCBI Genbank database with the accession numbers MN266903-MN266909 and MN530973-MN530976. These SNP may have an effect on economic (such as body composition) and carcass traits, reproduction, and milk yield and content in water buffalo populations and may prove to be useful for water buffalo breeding.
Animals
The present study has been one of the first attempts to thoroughly examine the effects of differe... more The present study has been one of the first attempts to thoroughly examine the effects of different kefir sources on fermentation characteristics, aerobic stability, and microbial communities of alfalfa silages. The effects of commercial kefir (CK) and homemade kefir culture (HK) applied with untreated a common control (CON) and three different application doses (5.0, 5.7, and 6.0 log cfu g−1) on wilted alfalfa and stored at an ambient temperature of 25–30 °C are studied. After 45 days of ensiling, fermentation characteristics and aerobic stability of silages were measured, and bacterial diversity was investigated by 16S ribosomal RNA gene sequencing using the GenomeLab™ GeXP platform. Both CK and HK accelerate more lactic acid production and reduced ammonia nitrogen concentration. Factor analysis of kefir sources suggests that the addition of kefir improves the aerobic stability of silages, even the initial water-soluble carbohydrate (WSC) content is inadequate via its antimicrobia...
Revue De Medecine Veterinaire, 2005
SUMMARY The Turkish native sheep breeds, possibly being the neighbours of the earliest domesticat... more SUMMARY The Turkish native sheep breeds, possibly being the neighbours of the earliest domesticated sheep populations, might be harbouring important genetic characteristics to be employed in the future for the improvement of sheep breeds. In order to design a conservation strategy, their genetic diversities must be determined. In the present study, based on three microsatellite loci, the genetic diversity of the Kivircik, Awassi, Akkaraman breeds (native) of Turkey as well as two of their crossbreeds Turkgeldi and Konya Merino were studied comparatively. It was observed that their heterozygoties are all high (0.6673-0.7822) compared to previously studied breeds, as expected for populations close to the center of domestication. Neighbour Joining (NJ) tree based on allele sharing distances indicated that the inertia of the breeds are not high. Yet, the genetic differentiations between the breeds based on pairwise FST (inbreeding coefficient) values are all significant. Furthermore, th...
In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Kara... more In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Karagül breeds of Anatolia, wild sheep Anatolian Mouflon (Ovis gmelinii anatolica) were used as the study material. Based on SRY and SRYM18 regions on the Y-chromosome, haplotypes of the populations were analyzed using DNA sequence analyses. The SRY region, A-oY1 allele was observed in all of the individuals studied. On the other hand, four different alleles corresponding to four Y-chromosome haplotypes were detected at the SRYM18 microsatellite region. Among native Anatolian breeds (n=143), H6 haplotype (80.41%), H4 haplotype (9.09%), H8 haplotype (8.40%) and H12 haplotype (2.1%) were identified. H6 haplotype was observed in all 16 individuals of Ovis gmelinii anatolica. Pairwise FST values based on haplotype frequencies were calculated for domestic sheep, and the highest FST value was observed between Karagül and Kıvırcık along with Karagül and Ovis gmelinii anatolica with pairwise FST val...
Revista Brasileira de Zootecnia
Animals : an Open Access Journal from MDPI, 2021
Simple Summary In the present study, twenty microsatellite loci were tested to assess and analyze... more Simple Summary In the present study, twenty microsatellite loci were tested to assess and analyze the genetic diversity between and within 17 different populations of Turkish water buffalo. The total number of animals sampled was 837, collected from six geographical regions: Marmara Region (MRM), Black Sea Region (BSR), Aegean Region (AER), Central Anatolia Region (CAR), Eastern Anatolia Region (EAR) and Southeastern Anatolia Region (SAR). All studied microsatellites markers showed allelic polymorphism. In this study, the results indicated a definite genetic diversity among the Turkish water buffalo populations which indicates the existence of at least two major clusters. Abstract The present study was aimed to investigate the genetic diversity among 17 Turkish water buffalo populations. A total of 837 individuals from 17 provincial populations were genotyped, using 20 microsatellites markers. The microsatellite markers analyzed were highly polymorphic with a mean number of alleles ...
Animals, 2021
In the present study, twenty microsatellite loci were tested to assess and analyze the genetic di... more In the present study, twenty microsatellite loci were tested to assess and analyze the genetic diversity between and within 17 different populations of Turkish water buffalo. The total number of animals sampled was 837, collected from six geographical regions: Marmara Region (MRM), Black Sea Region (BSR), Aegean Region (AER), Central Anatolia Region (CAR), Eastern Anatolia Region (EAR) and Southeastern Anatolia Region (SAR). All studied microsatellites markers showed allelic polymorphism. In this study, the results indicated a definite genetic diversity among the Turkish water buffalo populations which indicates the existence of at least two major clusters.