mehrzad jafari | Yasuj University of Medical Sciences (original) (raw)

Papers by mehrzad jafari

Introduction & Objective: Effective materials from Valerian officinalis L. have too much usag... more Introduction & Objective: Effective materials from Valerian officinalis L. have too much usage in the pharmacological industry. It is used as a sedative, anticonvulsion, and antidepressant drug. Serotonin has a widespread role in vital function such as sleep, awareness and calmness. In this study we evaluated the effect of hydrochloric extract of valerian on number and size of raphe magnus neurons in adult rat. Materials & Methods: In this experimental study, which was conducted at Yasuj University of Medical Sciences in 2009, forty adult Wistar rats, each 170-250 gr, were divided randomly into four groups (one control group and three experimental groups). The animals were injected daily for one month with doses of 300, 400 and 600 mg/kg of the extract. The control group just received distilled water. After transcardial perfusion, the whole brain was separated, then 10 μm sections of the brain stem were prepared, and hematoxylin and eosin (H&E) staining were done. Number and size of raphe magna neurons were observed under light microscope. The gathered data were analyzed by the SPSS software using One-way ANOVA and LSD. Results: The control group did not statistically show significant changes in number of raphe magna neurons. Comparison of the means of long and short diameter neurons showed significant increases in experimental groups with control group (P<0.05). In experimental groups the neuron nucleuses were more euchromatic than the control group. Conclusion: Hydrochloric extract of valerian has no effect on raphe magnus neurons, but it is effective on neurons' size. It can be concluded that the extract increases both neurons activity and serotonin secretion.

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2018

Health Scope

Background: Today, wireless communication systems are destructive with increased lipid peroxidati... more Background: Today, wireless communication systems are destructive with increased lipid peroxidation and oxidation state and have adverse biological effects on human health. Objectives: In this study, we examined the effects of exposure to WiFi wireless frequency (2.4 GHz) on histopathological changes in the cardiovascular system of rats. Methods: The experimental groups included 32 adult male rats divided into control (not exposed to heat and WiFi), WiFi (exposed to 2.45 GHz for 52 consecutive days (2 h/day)), heat (water bath of 43°C for 52 consecutive days (10 min/day)), and heat+WiFi groups (exposed to 2.45 GHz then water bath of 43°C). On the 52nd day, the heart was removed, and its total volume and weight were determined using stereological techniques. The number of cardiomyocytes nuclei and the volume of the myocardium were determined. Blood samples were collected to measure reduced glutathione (GSH) content, Total Antioxidant Capacity (TAC), and malondialdehyde level (MDA). D...

Avicenna Journal of Phytomedicine, 2015

Objective: To evaluate the hepatoprotective properties of Otostegia persica (O. persica) ethanol ... more Objective: To evaluate the hepatoprotective properties of Otostegia persica (O. persica) ethanol extract on carbon tetrachloride-induced liver damage in rats. Materials and Methods: Fifty adult male Wistar rats were randomly divided into five groups. Group I served as normal control and was given only olive oil intraperitoneally (i.p.). Group II, III, IV, and V were administered CCl4 mixed with olive oil 1:1 (1 ml/kg) i.p., twice a week for 8 weeks. Group II was maintained as CCl4-intoxicated control (hepatotoxic group). Group III, IV, and V received O. persica extract at a dose of 40, 80, and 120 mg/kg for 8 weeks every 48 h orally, respectively. Biochemical parameters including aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), albumin (ALB), total protein (TP), and lipid peroxidation marker (Malonaldialdehyde, (MDA) were determined in serum. After 8 weeks, animals were sacrificed, livers dissected out, and evaluated for his...

Avicenna Journal of Phytomedicine, 2016

Objective: The present study was conducted to investigate the hepatoprotective activity of hydro-... more Objective: The present study was conducted to investigate the hepatoprotective activity of hydro-ethanolic fruit extract of Rosa canina (R. canina) against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Materials and Methods: Male Wistar albino rats were randomly divided into six groups of 8 animals of each, including control, toxic (CCl4), R. canina 250, 500, and 750 mg/kg + CCl4 and R. canina 750 mg/kg alone. R. canina (p.o., daily) and CCl4 (1 ml/kg twice a week, 50% v/v in olive oil, i.p.) were administered to animals for six weeks. Serum analysis was performed to assay the levels of aspartate aminotransferase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP), albumin (ALB), total protein (TP) and malondialdehyde (MDA). Biochemical observations were also supplemented with histopathological examination (haematoxylin and eosin staining) of liver section. Results: Hepatotoxicity was evidenced by considerable increase in serum levels of AST, ALT, ALP, ...

Basic and Clinical Neuroscience Journal, 2018

Introduction: Peripheral nerve injury is one of the most common damages that lead to physical dis... more Introduction: Peripheral nerve injury is one of the most common damages that lead to physical disability. Due to the similarity between the coatings of skeletal muscles with nerves fiber, this research conducted to determine the effect of muscle graft with nerve growth factor (NGF) and laminin (L) on nerve repair. Methods: 10 mm sciatic nerve was transected and removed in forty-two female Wistar rats (200± 250 g) and equally divided into three groups. In the muscle graft +NGF + laminin group degenerated skeletal muscle was sutured with proximal and distal ends of the transected sciatic nerve. Then, NGF (100 ng) and laminin (1.28 mg/ml) were injected into the muscle graft. In the muscle graft group, normal saline were injected into the muscle graft. In the control group the 10 mm sciatic nerve removed without any treatment. Functional recovery was assessed based on sciatic functional index (SFI), tracing and histological study was achieved to assess nerve repair. Data was analyzed by ANOVA tests. Results: The Mean SFI significantly increased in the muscle graft +NGF + laminin (-76.6± 2.9) and muscle graft (-82.1± 3.5) groups in the 60 days post injury compared to the control group. The mean number of Labeled motor neuron significantly increased in the muscle graft +NGF + laminin (78.6± 3.1) and muscle graft (61.3± 6.1) groups compared to the control group (P<0.001). Mean number of myelinated axons in the distal segments of the muscle graft +NGF + laminin increased significantly compare to the muscle graft group. Conclusion: These findings suggest that muscle graft accompanying exogenous NGF and laminin provide clear therapeutic effects on nerve repair.

Immunopharmacology and Immunotoxicology, 2017

Pramipexole is a dopamine (DA) agonist (D2 subfamily receptors) that widely use in the treatment ... more Pramipexole is a dopamine (DA) agonist (D2 subfamily receptors) that widely use in the treatment of Parkinson's diseases. Some epidemiological and genetic studies propose a role of inflammation in the pathophysiology of Parkinson's disease. To our knowledge, there is no study regarding the anti-inflammatory activity of pramipexol. Therefore, the aim of the study was to investigate antiinflammatory effect of pramipexol. Anti-inflammatory effects of pramipexole were studied in three well-characterized animal models of inflammation, including carrageenan-or formalin-induced paw inflammation in rats, and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. The animals received pramipexol (0.25, 0.5 and 1 mg/kg, I.P.) 30 min before subplantar injection of carrageenan or formalin. Pramipexol (0.5 and 1 mg/kg) was also injected 30 min before topical application of TPA on the ear mice. Serum malondialdehyde (MDA) levels were evaluated in the carrageenan test. Finally, pathological examination of the inflamed tissues was carried out. Pramipexole significantly inhibited paw inflammation 1, 2, 3 and 4 h after carrageenan challenge compared with the control group (p < .001). Pramipexol also showed considerable anti-inflammatory activity against formalin-evoked paw edema over a period of 24 h (p < .001). TPA-induced ear edema was markedly decreased by pramipexol (p < .001). The pathological evaluation of the paws and ears revealed that pramipexole reduced tissue injury, neutrophil infiltration, and subcutaneous edema. Pramipexole did not alter the increased serum levels of MDA due to carrageenan injection. These data clearly indicate that pramipexol possesses significant anti-inflammatory activity. It seems that its antioxidants do not play an important role in these effects.

Diabetes is a metabolic disorder which can be diagnosed by the hyperglycemia resulting from defec... more Diabetes is a metabolic disorder which can be diagnosed by the hyperglycemia resulting from defects in insulin secretion and performance or both. Diabetes reduces the process of spermatogenesis and makes this phenomenon disordered. The objective of the present study is to investigate the effects of Aloe Vera extract on the production and quality of sperms of male diabetic rats. In the study, 24 diabetic adult male Wistar rats each with a weight of about 200-250 gr were provided. Rats became diabetic by the injection of 50 mg / kg/IPstreptozotocin. Then, rats divided into four groups randomly: the first group: diabetic control group which received daily distilled water orally, the second group: the diabetic group which received daily 400 mg / kg Aloe Vera extract orally. The third group, the healthy control group which daily distilled water orally, and the fourth group: the healthy group received daily 400 mg / kg distilled water orally. After 30 days of treatment, the rats were weig...

The aim of present study was determination of prevalence of iron deficiency in Yasuj BOY'S pr... more The aim of present study was determination of prevalence of iron deficiency in Yasuj BOY'S primary schools. A randomized, cross-sectional study was carried out on 280 boy's primary schools 6 to 10 year's old living in urban areas of Yasuj district in Kohgilooyeh and Boyrahmad Province of Iran. Two ml of blood in EDTA tubes for hematological parameters such as red blood cell (RBC) count, hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) was collected. Four ml blood in non anticoagulated tubes for serum iron (SI), total iron binding capacity (TIBC) and serum ferritin (SF) was collected also from all participants by antecubital vein puncture. Student's " t " test was used to compare the proportions iron deficiency and normal students in all parameters. In present research the prevalence of iron deficiency was 13.92%.The mean serum SI and SF were 105±33.2 µg/dL and 19.2± 12.5 ng /ml respectively. From 56 part...

Burn is one of the major causes of mortality in the world, and its prevalence in the developing c... more Burn is one of the major causes of mortality in the world, and its prevalence in the developing countries is multiple times the developed countries.In traditional medicinal plants were used to treat injuries and subside pains and accelerate the healing of burn wounds.Oak (Jaft) and Hypericum Perforatum as antioxidants have antimicrobial and antiseptic effects, the extracts of which were used in this design to repair second grade burns in male rats.Materials and methods:Some 50 male rats of wistar race with a weight of 200-250 grams were purchased, and were kept in the nest for one week for adaptation, with open (free) access to water, food, and appropriate ventilation, then the rats were divided randomly in five groups. The first group, burn control which were washed daily with physiology serum. The second, third, fourth, and fifth groups were treated after burn with Oak (Jaft) extract, Hypericum Perforatum, a mixture of Oak (Jaft) and Hypericum Perforatum, and silver-sulfadiazine o...

Iranian journal of radiation research (IJRR)

Background: Wi-Fi allows electronic devices such as laptops to exchange data or connect to a netw... more Background: Wi-Fi allows electronic devices such as laptops to exchange data or connect to a network resource. The main goal of this study was to assess the bioeffects of short term exposure to 2.4 GHz microwave radiation emitted from a common Wi-Fi router on sperm quality. Materials and Methods: Wistar rats were divided into 7 groups; I, sham exposed 4 h/day for 7days at 30 cm from the router. II, exposed for 2 h/day for 7days at 30 cm and sacrificed after 53 days. The exposure protocols for groups III to VII were 2h – 30 cm, 2h – 60 cm, 4 h – 30 cm, 4 h- 60 cm, 4 h – 30 cm (no data exchange) respectively. Rats in all the groups except group II, were sacrificed immediately after exposure and basic parameters of testicles weight, sperm motility, morphology, count, and DNA fragmentation were studied. Non-parametric tests were used to detect statistically significant differences between different groups. Results: Findings of this study showed statistically significant differences betw...

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2014

Iranian journal of reproductive medicine, 2014

Date Palm Pollen (DPP) and Astragalus genus are used in some countries for the treatment of infer... more Date Palm Pollen (DPP) and Astragalus genus are used in some countries for the treatment of infertility. This study was designed to investigate effects of DPP and Astragalus ovinus (A.Ovinus) on fertility in healthy adult male rats. Thirty-six rats were divided into six groups (n=6) including control and five treatment groups. DPP (120, 240 and 360 mg/kg) and A.ovinus (100, 500 mg/ kg) were orally given to the treatment groups. After thirty-five days, blood samples were taken to determine serum levels of FSH, LH, testosterone and estradiol. Weight of testis and epididymis, sperm count, sperm motility, seminiferous tubules diameter (STD), germinal cell layer thickness (GCLT), sertoli, leydig and spermatogonia cells were also evaluated. DPP at the of 120 and 240 mg/kg doses significantly raised the ratio of testis or epididymis to body weight, sperm count, sperm motility , and estradiol level compared to the control group (p<0.05). LH and testosterone levels only noticeably increas...

Breast cancer is among the most common causes of cancer deaths in women. Recently, phytochemicals... more Breast cancer is among the most common causes of cancer deaths in women. Recently, phytochemicals and their effects on the prevention and treatment of cancer have drawn a lot of attention. According to previous research, Matricaria chamomilla is a source of phytochemicals that have anticancer effects. In this research, the cytotoxic effects of the hydroalcoholic extract of Matricaria chamomilla root on the MCF-7 breast cancer cell line were investigated. Materials and Methods: The MCF-7 cells were treated for 24, 48 and 72 hours with different concentrations (200 ml g / -2000 ml g / ) of Matricaria chamomilla root extract in DMEM (Dulbecco's Modified Eagle's Medium) culture medium containing of Fetal Bovine Serum 10% (FBS). The half maximal inhibitory concentration (IC 50) was assayed by MTT method. Cell colony formation was also determined in percentage through a clonogenic assay. In this assay, a total of 1000 cells of the MCF-7 cell line were put in the wells of a 6-well plate and after 24 hours every well was treated with dosages of extract varying from 100 to 800 (ml g / ). Nine days following the treatment, the percentage of colony formation was measured. Results: The MCF-7 cells exposed to different dosages of Matricaria chamomilla root extract demonstrated 50% cell death after 24, 48 and 72 hours with 1954  4.2 (µg/ml), 1700  5.1 (µg/ml), and 1560  5.3 (µg/ml) dosages, respectively. In addition, a drop in cell colony formation was observed. Conclusion: The results indicated that Matricaria chamomilla root extract is cytotoxic to the MCF-7 breast cancer cell line. In order to study the effects of Matricaria chamomilla root extract on cell colony formation a clonogenic assay was performed. The assay revealed a decline in colony formation. Seemingly, Matricaria chamomilla root extract can contribute to the prevention or treatment of breast cancer. Hence, more studies can be conducted to use proper dosages of active ingredients of this substance for prevention or treatment of breast cancer.

Iranian Red Crescent Medical Journal, 2014

Pharmaceutical Biology, 2013

Nasturtium officinale R. Br. (watercress) has long been used in Iranian folk medicine to treat hy... more Nasturtium officinale R. Br. (watercress) has long been used in Iranian folk medicine to treat hypertension, hyperglycemia, and renal colic. Moreover, anticancer, antioxidant, and hepatoprotective properties of N. officinale have been reported. In this study, anti-inflammatory activity of the hydro-alcoholic extract from aerial parts of N. officinale was investigated. Oral administration of the hydro-alcoholic extract of N. officinale (250, 500 and 750 mg kg(-1)) was investigated on two well-characterized animal models of inflammation, including carrageenan- or formalin-induced paw edema in rats. Then, the topical anti-inflammatory effect of N. officinale (2 and 5 mg/ear) was studied on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. Finally, biopsy of the paw or ear was performed for pathological evaluation. Acute toxicity tests of N. officinale in rats established an oral LD50 of &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;5 g kg(-1). The extract of watercress (250, 500 and 750 mg kg(-1)) significantly inhibited carrageenan-induced paw edema 1, 2, 3 and 4 h after carrageenan challenge (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). The extract (500 mg kg(-1)) also showed considerable activity against formalin-evoked paw edema over a period of 24 h (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Furthermore, topical application of N. officinale (5 mg/ear) reduced TPA-induced ear edema (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Histopathologically, the extract decreased swelling and the tissue damage induced by carrageenan or TPA. Our findings indicate potent anti-inflammatory activity of N. officinale in systemic and topical application and propose its potential as an anti-inflammatory agent for treatment of inflammatory conditions.

Ovary is a part of the reproductive system can be affected by diabetes. Aloe vera is a plant that... more Ovary is a part of the reproductive system can be affected by diabetes. Aloe vera is a plant that reduces hypoglycemic. Thus, in present study, its aloe vera effect on ovaries development in during gestational age of 18 day embryo of rat. Material and methods: Two Groups of female Sprag dawlly rats were injected by streptozotocin (50 mg/kg/IP) and mating by male rat, vaginal plaque is objected. One groups of rats received aloe vera extract (400mg/kg) by oral in gestational age during and other group giving distilled water. After 18 day, rats sacrificed and their embryo were removed. Abdominal sections of embryo were processed and embedding by paraffin. The 5-6 µ sections were made and staining by hematoxyline-eosin and mason trichorome. Cellular of ovary change is evaluated. Results: Mean body weight do not show significantly (p>0.05). Mean blood sugar were decreased significantly (p<0.05). The mean of primordial germ cells were seen significant (p<0.05). Discussion: This study showed that Aloe Vera can reduce the blood sugar, also cause be increased primordial germ cells in embryos of diabetic mothers. [Mehrzad Jafari Barmak, Zabihollah Khaksar, Asghar Sharifi, Reza Mahmoudi. Effect of Aloe vera extract on ovaries development in during gestational age of 18 day embryo of diabetic rats.

Basic and clinical neuroscience, 2016

Prenatal stress has deleterious effects on the development of the brain and is associated with be... more Prenatal stress has deleterious effects on the development of the brain and is associated with behavioral and psychosocial problems in childhood and adulthood. This study aimed to determine the protective effect of L-arginine on fetal brain under maternal stress. Twenty pregnant Wistar rats (weighting 200-230 g) were randomly divided into 4 groups (n=5 for each group). The first nonstress and stress groups received 2 mL of normal saline and the other nonstress and stress two groups received L-arginine (200 mg/kg, IP) from their 5(th) to 20(th) days of pregnancy. The pregnant rats were killed on 20(th) day and the brain fetuses removed and prefrontal cortical thickness, total neurons in the prefrontal cortex and in the areas of CA1, CA2, and CA3 of the hippocampus were measured and counted. Nitrite levels in the brain were measured as an indicator for nitric oxide (NO) level. There was a significant decrease of mean number of pyramidal cells in the CA1 in prenatal stress group compar...

Plants and their extracts have considerable potential for wound healing. So, The aim of this stud... more Plants and their extracts have considerable potential for wound healing. So, The aim of this study was to evaluate the wound healing effect of combined hydroalcoholic extract of Teocurium Polium (TP) and the seed hull of Quercus brantii (Persian oak) (QB) and comparing with silver sulfadiazine (SSD) ointment (1%) in rats. Animals were divided into six groups consist of normal (untreated), physiologic serum, TP, seed hulls of Quercus brantii (QB), combination of TP and QB and the other group was SSD. Burn area size, epidermal and dermal diameter, number of hair follicle, histopathology of the burn skin and concentration of plasma malondialdehide (MDA) were determined in different groups. Animals treated with QB and TP, SSD and combination of two extracts (QB +TP) showed a significant reduction in the burn area. Serum MDA decreased significantly in animals treated with QB, TP and QB+TP. Histological comparison has shown that TP, QB and combination of them significantly increased re-epithelialization in burn wounds, as compared to normal group. In conclusion, our results demonstrated that topical application of TP and QB has a significant effect on the burn wound healing.