Tri Vien | National Yang Ming Chiao Tung University (original) (raw)

Papers by Tri Vien

BackgroundDengue fever and Zika are two of theAedes-borne diseases. Despite being widely used, sy... more BackgroundDengue fever and Zika are two of theAedes-borne diseases. Despite being widely used, synthetic mosquitocides become abortive for the mosquito control due to growing resistance and environmental pollution. In Gia Lai province (dengue-endemic area), a huge amount of cashew nut shell waste with roughly 100,000 tons/year has been disposed of into the environment, potentiating a high risk of pollution.Methodology/Principal findingsTo utilize it, anacardic acid was extracted and combined it with ethanol extract of the local lime peel, which contains limonene, to generate APL formulation. APL robustly exhibited inhibition of egg hatching, larvicidal effect, and repellent effect against female mosquitoes from oviposition sites in the laboratory and field. The results showed that, at a dose of 12.5 ppm, the APL formulation after 24 hours of treatment demonstrated oviposition deterrence againstAe. aegypti(43.6%) andAe. albopictus(59.6%); inhibited egg hatching ofAe. aegypti(49.6%) a...

Natural Product Communications, 2022

Anacardic acid (AA) is a natural active ingredient that accounts for 65% of the liquid extract fr... more Anacardic acid (AA) is a natural active ingredient that accounts for 65% of the liquid extract from the shell of the cashew nut. Due to the stronger cytotoxic activity of hydrogenated AA (HAA) against NTERA-2 cancer stem cells (CSCs) than AA itself, HAA was coconjugated with CD133 monoclonal antibody (mAb^CD133) into nanoliposomal particles (AMC). This nanoliposomal complex is expected to improved HAA activities against CSCs based on the targeting capacity of mAb^CD133 toward CD133, a typical CSCs' surface marker. AMC was manufactured with a mean size of 100.9 nm, a zeta potential of −40.7 mV, and a PDI of 0.283. We report a 100% encapsulation efficiency of HAA into liposomes and a 90.7% conjugation efficiency with mAb^CD133. The penetration of AMC into NTERA-2 CSCs after 2 h was 83.7%. The AMC complex inhibited NTERA-2 growth with an IC 50 (inhibition concentration at 50%) value of 75.83 ± 6.70 µM, showing the targeting ability and lower toxicity (IC 50 > 100 µM) on healthy cells. The AMC nanoparticles also demonstrated significant potential apoptotic induction by activating caspase 3 activity by up to 2.57 and 2.06 folds compared to that of the negative control at 20 and 4 µM, respectively. This induction was significant improvement in comparison with that of unconjugated HAA (P < .05). AMC presented a clear effect on the solid structure of NTERA-2 spheroids and significantly suppressed the proliferation of CSCs in the 3D tumorspheres with an IC 50 = 64.25 ± 3.15 µM, compared to the free form with an IC 50 = 82.22 ± 0.65 µM (P < .05). Therefore, this nanoliposomal complex exhibits promising capacities as an effective material against NTERA-2 CSCs.

Journal of Positive Psychology and Wellbeing, 2021

COVID-19 is a global health threat and an international public health emergency. Right awareness,... more COVID-19 is a global health threat and an international public health emergency. Right awareness, good knowledge, positive attitude, and good practice are one of the central issues to ensure the successful prevention and control of the COVID-19 pandemic. However, in some remote rural areas with difficult living conditions, social media about COVID-19 has not reached them. So, this study aims to present a possible solution to improve their awareness about COVID-19 among ethnic minorities in Gia Lai province, Vietnam. This study was conducted with 674 people in the community from September 1 to December 20, 2021. An intervention design using the stratified communication model and structured questionnaire was used to collect information regarding sociodemographic characteristics, knowledge (eight questions), attitudes (three questions), and practice (three questions). Of the total study participants, 64.31% were female and 95% lived in rural areas. The results showed that the poor knowledge, attitude and practice of the before intervention group improved compared to the after intervention and compared with the control group significantly increased with P < 0.05. Therefore, health education programs to improve awareness and raise KAP about COVID-19 are essential, especially for illiterate people, poor people, and a lack of means to communicate.

AIMS Bioengineering, 2020

Drought stress memory in plant can alter their physiological, biochemical and molecular to a subs... more Drought stress memory in plant can alter their physiological, biochemical and molecular to a subsequent stress. An experiment was conducted to determine biochemical parameters of soybean seedlings under drought stress memory. 14-days-old soybean seedlings were subjected to three consecutive water deficit phases (D1, D2, D3), each phase recovered by re-watering (R1, R2, R3), and control plant watering daily (R0). Leave of seedlings from these phases were collected and analyzed. Significantly increasing contents of soluble sugar and proline observed at the first drought stress. After the first re-water and continuous stress phases, these indicator contents decreased and maintained at a relatively stable level. Expression level of two memory genes encoded to transcription factor (NAC09 and NAC109) and one gene encoded enzyme Δ1-pyrroline-5-carboxylate synthetase (P5CS1) were increased in the first stress and decreased in third stress. Our results demonstrate that changing of biochemical parameters of soybean seedlings can be seen as the strong indications of "drought stress memory". This result may serve as a reference platform to study advanced researches at molecular and genetic levels.

BackgroundDengue fever and Zika are two of theAedes-borne diseases. Despite being widely used, sy... more BackgroundDengue fever and Zika are two of theAedes-borne diseases. Despite being widely used, synthetic mosquitocides become abortive for the mosquito control due to growing resistance and environmental pollution. In Gia Lai province (dengue-endemic area), a huge amount of cashew nut shell waste with roughly 100,000 tons/year has been disposed of into the environment, potentiating a high risk of pollution.Methodology/Principal findingsTo utilize it, anacardic acid was extracted and combined it with ethanol extract of the local lime peel, which contains limonene, to generate APL formulation. APL robustly exhibited inhibition of egg hatching, larvicidal effect, and repellent effect against female mosquitoes from oviposition sites in the laboratory and field. The results showed that, at a dose of 12.5 ppm, the APL formulation after 24 hours of treatment demonstrated oviposition deterrence againstAe. aegypti(43.6%) andAe. albopictus(59.6%); inhibited egg hatching ofAe. aegypti(49.6%) a...

Natural Product Communications, 2022

Anacardic acid (AA) is a natural active ingredient that accounts for 65% of the liquid extract fr... more Anacardic acid (AA) is a natural active ingredient that accounts for 65% of the liquid extract from the shell of the cashew nut. Due to the stronger cytotoxic activity of hydrogenated AA (HAA) against NTERA-2 cancer stem cells (CSCs) than AA itself, HAA was coconjugated with CD133 monoclonal antibody (mAb^CD133) into nanoliposomal particles (AMC). This nanoliposomal complex is expected to improved HAA activities against CSCs based on the targeting capacity of mAb^CD133 toward CD133, a typical CSCs' surface marker. AMC was manufactured with a mean size of 100.9 nm, a zeta potential of −40.7 mV, and a PDI of 0.283. We report a 100% encapsulation efficiency of HAA into liposomes and a 90.7% conjugation efficiency with mAb^CD133. The penetration of AMC into NTERA-2 CSCs after 2 h was 83.7%. The AMC complex inhibited NTERA-2 growth with an IC 50 (inhibition concentration at 50%) value of 75.83 ± 6.70 µM, showing the targeting ability and lower toxicity (IC 50 > 100 µM) on healthy cells. The AMC nanoparticles also demonstrated significant potential apoptotic induction by activating caspase 3 activity by up to 2.57 and 2.06 folds compared to that of the negative control at 20 and 4 µM, respectively. This induction was significant improvement in comparison with that of unconjugated HAA (P < .05). AMC presented a clear effect on the solid structure of NTERA-2 spheroids and significantly suppressed the proliferation of CSCs in the 3D tumorspheres with an IC 50 = 64.25 ± 3.15 µM, compared to the free form with an IC 50 = 82.22 ± 0.65 µM (P < .05). Therefore, this nanoliposomal complex exhibits promising capacities as an effective material against NTERA-2 CSCs.

Journal of Positive Psychology and Wellbeing, 2021

COVID-19 is a global health threat and an international public health emergency. Right awareness,... more COVID-19 is a global health threat and an international public health emergency. Right awareness, good knowledge, positive attitude, and good practice are one of the central issues to ensure the successful prevention and control of the COVID-19 pandemic. However, in some remote rural areas with difficult living conditions, social media about COVID-19 has not reached them. So, this study aims to present a possible solution to improve their awareness about COVID-19 among ethnic minorities in Gia Lai province, Vietnam. This study was conducted with 674 people in the community from September 1 to December 20, 2021. An intervention design using the stratified communication model and structured questionnaire was used to collect information regarding sociodemographic characteristics, knowledge (eight questions), attitudes (three questions), and practice (three questions). Of the total study participants, 64.31% were female and 95% lived in rural areas. The results showed that the poor knowledge, attitude and practice of the before intervention group improved compared to the after intervention and compared with the control group significantly increased with P < 0.05. Therefore, health education programs to improve awareness and raise KAP about COVID-19 are essential, especially for illiterate people, poor people, and a lack of means to communicate.

AIMS Bioengineering, 2020

Drought stress memory in plant can alter their physiological, biochemical and molecular to a subs... more Drought stress memory in plant can alter their physiological, biochemical and molecular to a subsequent stress. An experiment was conducted to determine biochemical parameters of soybean seedlings under drought stress memory. 14-days-old soybean seedlings were subjected to three consecutive water deficit phases (D1, D2, D3), each phase recovered by re-watering (R1, R2, R3), and control plant watering daily (R0). Leave of seedlings from these phases were collected and analyzed. Significantly increasing contents of soluble sugar and proline observed at the first drought stress. After the first re-water and continuous stress phases, these indicator contents decreased and maintained at a relatively stable level. Expression level of two memory genes encoded to transcription factor (NAC09 and NAC109) and one gene encoded enzyme Δ1-pyrroline-5-carboxylate synthetase (P5CS1) were increased in the first stress and decreased in third stress. Our results demonstrate that changing of biochemical parameters of soybean seedlings can be seen as the strong indications of "drought stress memory". This result may serve as a reference platform to study advanced researches at molecular and genetic levels.