Arigue Dessouky | Zagazig University (original) (raw)

Papers by Arigue Dessouky

Annals of Anatomy-anatomischer Anzeiger, Jul 1, 2023

Mansoura Journal of Forensic Medicine and Clinical Toxicology, 2020

Chlorpyrifos (CPF) can induce ovarian damage and reproductive dysfunction through oxidative stres... more Chlorpyrifos (CPF) can induce ovarian damage and reproductive dysfunction through oxidative stress mechanisms. These can be reversed by antioxidants such as Epigallocatechin-3-gallate (EGCG) in green tea. This study aimed to investigate the toxicity effects of CPF on rat ovaries and the effectiveness of EGCG to protect against these effects. Fifty adult female albino rats were randomly assigned to five equal groups; negative and positive control groups, EGCG-treated group, CPFtreated group and CPF+EGCG-treated group. After four weeks the rat body weight and relative ovarian weight were estimated, and blood samples were collected to assess reproductive hormones (RHs) levels. Evaluation of ovarian oxidative stress indicators malondialdhyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) was performed. Ovarian sections were prepared and examined using the hematoxylin and eosin stain, proliferating cell nuclear antigen, and morphometric study to assess histopathologic changes. CPFtreated rats had significant lower body weight and relative ovarian weight compared to controls (P<0.001) and those treated with CPF+EGCG (P<0.001). CPF induced significant reduction in RHs levels and ovarian SOD, GSH and CAT activities when given alone, which were improved by the combined administration of CPF and EGCG. The levels of ovarian MDA and NO were significantly higher in CPF-treated rats than controls and rats treated with CPF+EGCG. The cellular proliferation in ovarian Graafian follicles was significantly suppressed in CPF-treated animals. Hence CPF can induce oxidative damage in rat ovaries, EGCG, a natural potent antioxidant, can be useful against the CPF toxicity effects in ovaries.

Cell and Tissue Research

Ozone (O3) gas is a double-sided weapon. It provides a shield that protects life on earth from th... more Ozone (O3) gas is a double-sided weapon. It provides a shield that protects life on earth from the harmful ultraviolet (UV) rays, but ground-level O3 is considered an urban air pollutant. So, a rat model of chronic O3 inhalation was established to assess the biochemical and morphological alterations in the lung tissue and to investigate the ameliorative effects of bone marrow–derived mesenchymal stem cells (BMSCs) with or without hypoxia pre-treatment. Forty-two adult male albino rats were divided into four groups: control, ozone-exposed, normoxic BMSC-treated, and hypoxic BMSC-treated groups. Lung tissue sections were processed for light and electron microscope examination, immunohistochemical staining for caspase 3, and iNOS. Quantitative real-time PCR for IL-1α, IL-17, TNF-α, and Nrf2 mRNA gene expression were also performed. Chronic O3 exposure caused elevated inflammatory cytokines and decreased antioxidant Nrf2 mRNA expression. Marked morphological alterations with increased c...

Egyptian Journal of Histology, 2021

Stem Cell Research & Therapy

Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian ... more Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian epithelial surface. They contribute to postnatal oogenesis as they have the ability to generate immature oocytes and resist the chemotherapy. These cells express markers of pluripotent embryonic and primordial germ cells. Objective We aimed to explore the capability of VSELs in restoring the postnatal oogenesis of chemo-ablated rat ovaries treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with pregnant mare serum gonadotropin (PMSG). Methods Female albino rats were randomly assigned across five groups: I (control), II (chemo-ablation), III (chemo-ablation + PMSG), IV (chemo-ablation + MSCs), and V (chemo-ablation + PMSG + MSCs). Postnatal oogenesis was assessed through measurement of OCT4, OCT4A, Scp3, Mvh, Nobox, Dazl4, Nanog, Sca-1, FSHr, STRA8, Bax, miR143, and miR376a transcript levels using qRT-PCR. Expression of selected key proteins were established as fur...

BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause o... more BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause on the sublingual salivary gland (SSG) and the potential therapeutic effect of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs). METHODS Thirty rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX), and ovariectomized stem cells injected (OVX + hUCB-MSCs). Expressions of α-SMA, AQP1, Sca-1, PCNA, ssDNA, and caspase-3 were determined. Homing of hUCB-MSCs was detected by fluorescence microscopy and examination of immunostained sections for human CD105 and CD34 were performed. Morphometric data were statistically analyzed using the Kruskal-Wallis test followed by Scheffé's method. Correlation of AQP1 with Sca-1-positive sublingual stem cells was also analyzed. RESULTS In the SSGs of the OVX group, ballooned mucus acinar cells, atrophied serous cells, and a decreased number and height of duct lining cells were observed. The interstitial space...

Cells Tissues Organs, 2020

Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypox... more Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypoxia-preconditioned human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on hypoglycemic testicular injury. We examined 56 testes from 28 animals: 7 rats with insulin-induced hypoglycemia (HG group), 7 hypoglycemic rats which received an intratesticular injection of hUCB-MSCs (HG-MSC group), and 14 untreated control rats. Testosterone level, testicular catalase (CAT) activity, and malondialdehyde (MDA) level were analyzed. Immunostaining for specific testicular germ and somatic cell markers was performed. Proliferating and apoptotic cells were detected by anti-PCNA and anti-caspase-3, respectively. Morphometrical data were statistically analyzed. The hypoglycemic rats showed a significant decrease in testosterone level and CAT activity and a significant increase in MDA production. Examination of histological structure and protein expression of diverse germ cell markers rev...

Nesrine Ebrahim1,2, Inas A. Ahmed3, 4, Noha I. Hussien5, Arigue A. Dessouky6, Ayman Samir Farid7,... more Nesrine Ebrahim1,2, Inas A. Ahmed3, 4, Noha I. Hussien5, Arigue A. Dessouky6, Ayman Samir Farid7,*, Amal M. Elshazly8, Ola Mostafa1, Walaa Bayoumie El Gazzar3, Safwa M. Sorour9, Yasmin Seleem9, Ahmed M. Hussein10, Dina Sabry11, 12 1Department of Histology and Cell Biology, 2Stem Cell Unit, 3Department of Medical Biochemistry, 4Molecular Biology and Biotechnology Unit, 5Department of physiology, Faculty of Medicine, Benha University, Benha, 13518, Qalyubia, Egypt.

BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause o... more BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause on the sublingual salivary gland (SSG) and the potential therapeutic effect of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs). METHODS Thirty rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX), and ovariectomized stem cells injected (OVX + hUCB-MSCs). Expressions of α-SMA, AQP1, Sca-1, PCNA, ssDNA, and caspase-3 were determined. Homing of hUCB-MSCs was detected by fluorescence microscopy and examination of immunostained sections for human CD105 and CD34 were performed. Morphometric data were statistically analyzed using the Kruskal-Wallis test followed by Scheffé's method. Correlation of AQP1 with Sca-1-positive sublingual stem cells was also analyzed. RESULTS In the SSGs of the OVX group, ballooned mucus acinar cells, atrophied serous cells, and a decreased number and height of duct lining cells were observed. The interstitial space...

Stem Cell Research & Therapy

Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus charac... more Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to the rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose-derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, and PRP+ADSCs-treated groups]. The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen, and angiogenesis. Moreover, gene and protein expression of key elements of the Notch signaling pathway (Notch1, Delta-like canonical Notc...

Cells Tissues Organs

Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypox... more Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypoxia-preconditioned human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on hypoglycemic testicular injury. We examined 56 testes from 28 animals: 7 rats with insulin-induced hypoglycemia (HG group), 7 hypoglycemic rats which received an intratesticular injection of hUCB-MSCs (HG-MSC group), and 14 untreated control rats. Testosterone level, testicular catalase (CAT) activity, and malondialdehyde (MDA) level were analyzed. Immunostaining for specific testicular germ and somatic cell markers was performed. Proliferating and apoptotic cells were detected by anti-PCNA and anti-caspase-3, respectively. Morphometrical data were statistically analyzed. The hypoglycemic rats showed a significant decrease in testosterone level and CAT activity and a significant increase in MDA production. Examination of histological structure and protein expression of diverse germ cell markers rev...

Stem Cell Research & Therapy

Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian ... more Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian epithelial surface. They contribute to postnatal oogenesis as they have the ability to generate immature oocytes and resist the chemotherapy. These cells express markers of pluripotent embryonic and primordial germ cells. Objective We aimed to explore the capability of VSELs in restoring the postnatal oogenesis of chemo-ablated rat ovaries treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with pregnant mare serum gonadotropin (PMSG). Methods Female albino rats were randomly assigned across five groups: I (control), II (chemo-ablation), III (chemo-ablation + PMSG), IV (chemo-ablation + MSCs), and V (chemo-ablation + PMSG + MSCs). Postnatal oogenesis was assessed through measurement of OCT4, OCT4A, Scp3, Mvh, Nobox, Dazl4, Nanog, Sca-1, FSHr, STRA8, Bax, miR143, and miR376a transcript levels using qRT-PCR. Expression of selected key proteins were established as fur...

Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus charac... more Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups (control, sham, diabetic, PRP-treated, ADSCs-treated and PRP+ADSCs-treated groups). The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen and angiogenesis. Moreover, gene and protein expression of key elements of Notch signaling pathway (Notch1, Delta like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes...

Egyptian Journal of Histology

Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent vi... more Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent visual impairment. In spite of great advances made in the treatment of these cases, the structural and functional restoration of corneal alkali burns remains challenging. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been demonstrated to enhance corneal healing. However the problem remains in finding a suitable vehicle to deliver BM-MSCs to the site of injury. Objectives: To evaluate the therapeutic potential of BM-MSCs loaded on two biological vehicles (amniotic membrane versus platelet-rich plasma gel) in corneal alkali burns. Materials & Methods: Fifty rats were randomly divided into five equal groups. Group I (control group), group II (corneal ulcer group) and group III (recovery group). Group IV rats with corneal ulcers were treated with amniotic membrane loaded with BM-MSCs while in the group V the rats with corneal ulcers were treated with platelet-rich plasma cells loaded with BM-MSCs. The corneal specimens were processed for the light microscope, transmission electron microscope examination, and RT qPCR assay for VEGF and iNOS. Results: Group II showed epithelial separation and denudation with disorganized stroma and disrupted Descemet's membrane and endothelium. Group III showed residual epithelial separation and vacuolation with neovascularization of the disorganized stroma. Group IV showed thinning of corneal epithelium with inflammatory infiltration of the stroma. Group V corneas revealed restoration of corneal epithelial thickness, organized stroma and continuous Descemet's membrane. Also, there was an upregulation of iNOS & VEGF gene expression in groups II and III which was downregulated in group IV and V. Conclusions: The combination of BM-MSCs with PRP gel was a promising treatment for corneal alkali burns and may be applicable for other types of corneal disorders.

Cells

Background: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common... more Background: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common cause of end-stage renal disease. Autophagy has a defensive role against kidney damage caused by hyperglycemia. Mesenchymal stem cell (MSC)-derived exosomes are currently considered as a new promising therapy for chronic renal injury. However, the renal-protective mechanism of exosomes on DN is not completely understood. We examined the potential role of MSC-derived exosomes for enhancement of autophagy activity and their effect on DN. In our study, we used five groups of rats: control; DN; DN treated with exosomes; DN treated with 3-methyladenine (3-MA) and chloroquine (inhibitors of autophagy); and DN treated with 3-methyladenine (3-MA), chloroquine, and exosome groups. We assessed renal function, morphology, and fibrosis. Moreover, ratios of the autophagy markers mechanistic target of rapamycin (mTOR), Beclin-1, light chain-3 (LC3-II), and LC3-II/LC3-I were detected. Additionally, el...

ejhistology.eg.net

Introduction: Renal development involves 2 basic processes: morphologic formation and acquisition... more Introduction: Renal development involves 2 basic processes: morphologic formation and acquisition of function. Aim of the Work: The aim of the present study was to obtain histological information about the postnatal development of the filtration barrier and ...

Annals of Anatomy-anatomischer Anzeiger, Jul 1, 2023

Mansoura Journal of Forensic Medicine and Clinical Toxicology, 2020

Chlorpyrifos (CPF) can induce ovarian damage and reproductive dysfunction through oxidative stres... more Chlorpyrifos (CPF) can induce ovarian damage and reproductive dysfunction through oxidative stress mechanisms. These can be reversed by antioxidants such as Epigallocatechin-3-gallate (EGCG) in green tea. This study aimed to investigate the toxicity effects of CPF on rat ovaries and the effectiveness of EGCG to protect against these effects. Fifty adult female albino rats were randomly assigned to five equal groups; negative and positive control groups, EGCG-treated group, CPFtreated group and CPF+EGCG-treated group. After four weeks the rat body weight and relative ovarian weight were estimated, and blood samples were collected to assess reproductive hormones (RHs) levels. Evaluation of ovarian oxidative stress indicators malondialdhyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) was performed. Ovarian sections were prepared and examined using the hematoxylin and eosin stain, proliferating cell nuclear antigen, and morphometric study to assess histopathologic changes. CPFtreated rats had significant lower body weight and relative ovarian weight compared to controls (P<0.001) and those treated with CPF+EGCG (P<0.001). CPF induced significant reduction in RHs levels and ovarian SOD, GSH and CAT activities when given alone, which were improved by the combined administration of CPF and EGCG. The levels of ovarian MDA and NO were significantly higher in CPF-treated rats than controls and rats treated with CPF+EGCG. The cellular proliferation in ovarian Graafian follicles was significantly suppressed in CPF-treated animals. Hence CPF can induce oxidative damage in rat ovaries, EGCG, a natural potent antioxidant, can be useful against the CPF toxicity effects in ovaries.

Cell and Tissue Research

Ozone (O3) gas is a double-sided weapon. It provides a shield that protects life on earth from th... more Ozone (O3) gas is a double-sided weapon. It provides a shield that protects life on earth from the harmful ultraviolet (UV) rays, but ground-level O3 is considered an urban air pollutant. So, a rat model of chronic O3 inhalation was established to assess the biochemical and morphological alterations in the lung tissue and to investigate the ameliorative effects of bone marrow–derived mesenchymal stem cells (BMSCs) with or without hypoxia pre-treatment. Forty-two adult male albino rats were divided into four groups: control, ozone-exposed, normoxic BMSC-treated, and hypoxic BMSC-treated groups. Lung tissue sections were processed for light and electron microscope examination, immunohistochemical staining for caspase 3, and iNOS. Quantitative real-time PCR for IL-1α, IL-17, TNF-α, and Nrf2 mRNA gene expression were also performed. Chronic O3 exposure caused elevated inflammatory cytokines and decreased antioxidant Nrf2 mRNA expression. Marked morphological alterations with increased c...

Egyptian Journal of Histology, 2021

Stem Cell Research & Therapy

Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian ... more Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian epithelial surface. They contribute to postnatal oogenesis as they have the ability to generate immature oocytes and resist the chemotherapy. These cells express markers of pluripotent embryonic and primordial germ cells. Objective We aimed to explore the capability of VSELs in restoring the postnatal oogenesis of chemo-ablated rat ovaries treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with pregnant mare serum gonadotropin (PMSG). Methods Female albino rats were randomly assigned across five groups: I (control), II (chemo-ablation), III (chemo-ablation + PMSG), IV (chemo-ablation + MSCs), and V (chemo-ablation + PMSG + MSCs). Postnatal oogenesis was assessed through measurement of OCT4, OCT4A, Scp3, Mvh, Nobox, Dazl4, Nanog, Sca-1, FSHr, STRA8, Bax, miR143, and miR376a transcript levels using qRT-PCR. Expression of selected key proteins were established as fur...

BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause o... more BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause on the sublingual salivary gland (SSG) and the potential therapeutic effect of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs). METHODS Thirty rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX), and ovariectomized stem cells injected (OVX + hUCB-MSCs). Expressions of α-SMA, AQP1, Sca-1, PCNA, ssDNA, and caspase-3 were determined. Homing of hUCB-MSCs was detected by fluorescence microscopy and examination of immunostained sections for human CD105 and CD34 were performed. Morphometric data were statistically analyzed using the Kruskal-Wallis test followed by Scheffé's method. Correlation of AQP1 with Sca-1-positive sublingual stem cells was also analyzed. RESULTS In the SSGs of the OVX group, ballooned mucus acinar cells, atrophied serous cells, and a decreased number and height of duct lining cells were observed. The interstitial space...

Cells Tissues Organs, 2020

Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypox... more Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypoxia-preconditioned human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on hypoglycemic testicular injury. We examined 56 testes from 28 animals: 7 rats with insulin-induced hypoglycemia (HG group), 7 hypoglycemic rats which received an intratesticular injection of hUCB-MSCs (HG-MSC group), and 14 untreated control rats. Testosterone level, testicular catalase (CAT) activity, and malondialdehyde (MDA) level were analyzed. Immunostaining for specific testicular germ and somatic cell markers was performed. Proliferating and apoptotic cells were detected by anti-PCNA and anti-caspase-3, respectively. Morphometrical data were statistically analyzed. The hypoglycemic rats showed a significant decrease in testosterone level and CAT activity and a significant increase in MDA production. Examination of histological structure and protein expression of diverse germ cell markers rev...

Nesrine Ebrahim1,2, Inas A. Ahmed3, 4, Noha I. Hussien5, Arigue A. Dessouky6, Ayman Samir Farid7,... more Nesrine Ebrahim1,2, Inas A. Ahmed3, 4, Noha I. Hussien5, Arigue A. Dessouky6, Ayman Samir Farid7,*, Amal M. Elshazly8, Ola Mostafa1, Walaa Bayoumie El Gazzar3, Safwa M. Sorour9, Yasmin Seleem9, Ahmed M. Hussein10, Dina Sabry11, 12 1Department of Histology and Cell Biology, 2Stem Cell Unit, 3Department of Medical Biochemistry, 4Molecular Biology and Biotechnology Unit, 5Department of physiology, Faculty of Medicine, Benha University, Benha, 13518, Qalyubia, Egypt.

BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause o... more BACKGROUND Ovariectomized menopausal rat model was used to investigate the effects of menopause on the sublingual salivary gland (SSG) and the potential therapeutic effect of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs). METHODS Thirty rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX), and ovariectomized stem cells injected (OVX + hUCB-MSCs). Expressions of α-SMA, AQP1, Sca-1, PCNA, ssDNA, and caspase-3 were determined. Homing of hUCB-MSCs was detected by fluorescence microscopy and examination of immunostained sections for human CD105 and CD34 were performed. Morphometric data were statistically analyzed using the Kruskal-Wallis test followed by Scheffé's method. Correlation of AQP1 with Sca-1-positive sublingual stem cells was also analyzed. RESULTS In the SSGs of the OVX group, ballooned mucus acinar cells, atrophied serous cells, and a decreased number and height of duct lining cells were observed. The interstitial space...

Stem Cell Research & Therapy

Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus charac... more Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to the rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose-derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, and PRP+ADSCs-treated groups]. The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen, and angiogenesis. Moreover, gene and protein expression of key elements of the Notch signaling pathway (Notch1, Delta-like canonical Notc...

Cells Tissues Organs

Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypox... more Hypoglycemia is a neglected metabolic disorder. Thus, we evaluated the protective effect of hypoxia-preconditioned human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on hypoglycemic testicular injury. We examined 56 testes from 28 animals: 7 rats with insulin-induced hypoglycemia (HG group), 7 hypoglycemic rats which received an intratesticular injection of hUCB-MSCs (HG-MSC group), and 14 untreated control rats. Testosterone level, testicular catalase (CAT) activity, and malondialdehyde (MDA) level were analyzed. Immunostaining for specific testicular germ and somatic cell markers was performed. Proliferating and apoptotic cells were detected by anti-PCNA and anti-caspase-3, respectively. Morphometrical data were statistically analyzed. The hypoglycemic rats showed a significant decrease in testosterone level and CAT activity and a significant increase in MDA production. Examination of histological structure and protein expression of diverse germ cell markers rev...

Stem Cell Research & Therapy

Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian ... more Background Very small embryonic-like stem cells (VSELs) are a rare population within the ovarian epithelial surface. They contribute to postnatal oogenesis as they have the ability to generate immature oocytes and resist the chemotherapy. These cells express markers of pluripotent embryonic and primordial germ cells. Objective We aimed to explore the capability of VSELs in restoring the postnatal oogenesis of chemo-ablated rat ovaries treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with pregnant mare serum gonadotropin (PMSG). Methods Female albino rats were randomly assigned across five groups: I (control), II (chemo-ablation), III (chemo-ablation + PMSG), IV (chemo-ablation + MSCs), and V (chemo-ablation + PMSG + MSCs). Postnatal oogenesis was assessed through measurement of OCT4, OCT4A, Scp3, Mvh, Nobox, Dazl4, Nanog, Sca-1, FSHr, STRA8, Bax, miR143, and miR376a transcript levels using qRT-PCR. Expression of selected key proteins were established as fur...

Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus charac... more Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups (control, sham, diabetic, PRP-treated, ADSCs-treated and PRP+ADSCs-treated groups). The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen and angiogenesis. Moreover, gene and protein expression of key elements of Notch signaling pathway (Notch1, Delta like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes...

Egyptian Journal of Histology

Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent vi... more Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent visual impairment. In spite of great advances made in the treatment of these cases, the structural and functional restoration of corneal alkali burns remains challenging. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been demonstrated to enhance corneal healing. However the problem remains in finding a suitable vehicle to deliver BM-MSCs to the site of injury. Objectives: To evaluate the therapeutic potential of BM-MSCs loaded on two biological vehicles (amniotic membrane versus platelet-rich plasma gel) in corneal alkali burns. Materials & Methods: Fifty rats were randomly divided into five equal groups. Group I (control group), group II (corneal ulcer group) and group III (recovery group). Group IV rats with corneal ulcers were treated with amniotic membrane loaded with BM-MSCs while in the group V the rats with corneal ulcers were treated with platelet-rich plasma cells loaded with BM-MSCs. The corneal specimens were processed for the light microscope, transmission electron microscope examination, and RT qPCR assay for VEGF and iNOS. Results: Group II showed epithelial separation and denudation with disorganized stroma and disrupted Descemet's membrane and endothelium. Group III showed residual epithelial separation and vacuolation with neovascularization of the disorganized stroma. Group IV showed thinning of corneal epithelium with inflammatory infiltration of the stroma. Group V corneas revealed restoration of corneal epithelial thickness, organized stroma and continuous Descemet's membrane. Also, there was an upregulation of iNOS & VEGF gene expression in groups II and III which was downregulated in group IV and V. Conclusions: The combination of BM-MSCs with PRP gel was a promising treatment for corneal alkali burns and may be applicable for other types of corneal disorders.

Cells

Background: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common... more Background: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common cause of end-stage renal disease. Autophagy has a defensive role against kidney damage caused by hyperglycemia. Mesenchymal stem cell (MSC)-derived exosomes are currently considered as a new promising therapy for chronic renal injury. However, the renal-protective mechanism of exosomes on DN is not completely understood. We examined the potential role of MSC-derived exosomes for enhancement of autophagy activity and their effect on DN. In our study, we used five groups of rats: control; DN; DN treated with exosomes; DN treated with 3-methyladenine (3-MA) and chloroquine (inhibitors of autophagy); and DN treated with 3-methyladenine (3-MA), chloroquine, and exosome groups. We assessed renal function, morphology, and fibrosis. Moreover, ratios of the autophagy markers mechanistic target of rapamycin (mTOR), Beclin-1, light chain-3 (LC3-II), and LC3-II/LC3-I were detected. Additionally, el...

ejhistology.eg.net

Introduction: Renal development involves 2 basic processes: morphologic formation and acquisition... more Introduction: Renal development involves 2 basic processes: morphologic formation and acquisition of function. Aim of the Work: The aim of the present study was to obtain histological information about the postnatal development of the filtration barrier and ...