Dina Alsadek | Zagazig University (original) (raw)

Papers by Dina Alsadek

Research paper thumbnail of Optimizing the Growth, Health, Reproductive Performance, and Gonadal Histology of Broodstock Fantail Goldfish (Carassius auratus, L.) by Dietary Cacao Bean Meal

Animals, 2020

The potential effects of cacao bean meal, Theobroma cacao L., (CBM) on the growth, health status,... more The potential effects of cacao bean meal, Theobroma cacao L., (CBM) on the growth, health status, blood biochemical parameters, antioxidant, immune status, physiological parameters, female reproductive performance, and gonadal histological features of fantail goldfish (Carassius auratus, L.) were evaluated using a complete randomized block design with sex as a block. The trial lasted for 60 days. A total of 54 healthy fantail goldfish (36 broodstock females and 18 broodstock males) were randomly allocated into three treatments with supplementation of three levels of cocoa powder 0, 5, and 10 g kg−1 diet, CBM0, CBM5, and CBM10, respectively, with the sex ratio being four females:two males per replicate. The body weight gain and feed conversion ratio of males were increased in the CBM10 treatment (p < 0.05). The CBM10 diet improved relative feed costs (p < 0.05). Females fed on the CBM10 diet had an increase in the serum level of total protein (p = 0.001). Females fed on a diet ...

Research paper thumbnail of Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection

Zagazig Veterinary Journal

The ultimate goal of cancer science is to learn how to select a cancer cell surface. Glycans in c... more The ultimate goal of cancer science is to learn how to select a cancer cell surface. Glycans in cancer cells are frequently at varying quantities or have fundamentally various structures than those seen in normal cells. The presence of sialic acid (Neu5Ac), which is prevalent at the end of glycan chains, is significant. N-glycolylneuraminic acid (Neu5Gc) is a "non-human" sugar type that intercept into Neu5Ac natural biosynthetic machinery, presaging as a perspective biomarker. Malignant breast cells resistance to chemotherapy and their non-malignant counterparts, when treated with the Neu5Gc under nutrient deprivation, display cell surface tumor-associated Neu5Gc-rich glycans, which is capture with Neu5Gc linkage specific plant lectins such as Triticum vulgaris agglutinin (WGA), Sambucus nigra agglutinin (SNA), and Maackia amurensis agglutinin I (MAL-I). Nevertheless, MAL-I binding differentiates surface of malignant breast cell line resistance to chemotherapy MCF-7/H compared to normal mammary epithelial cell line MCF-10/A. These findings emphasize the importance of one oxygen atom in Neu5Gc; it's supplementing of nutrient-depleted cancer cells' resistance to chemotherapy and facilitates the way for the implementation of better diagnostic and prognostic approaches.

Research paper thumbnail of Infoguides BUS110 Euripides Home

Research paper thumbnail of An automated pipeline for studying binding affinity of SARS-CoV-2 variants and the human ACE2 receptor

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research, 2021

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research, 2021

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Liver-Specific Stem Cells and Hepatocellular Carcinoma: An in Vitro Comparison Between Stem Cells Isolated from Malignant and Non- Malignant Human Liver Tissue

Journal of the Medical Research Institute, 2016

Research paper thumbnail of Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation

Biomaterials, 2015

Cancer is characterized by abnormal energy metabolism shaped by nutrient deprivation that maligna... more Cancer is characterized by abnormal energy metabolism shaped by nutrient deprivation that malignant cells experience during various stages of tumor development. This study investigated the response of nutrient-deprived cancer cells and their non-malignant counterparts to sialic acid supplementation and found that cells utilize negligible amounts of this sugar for energy. Instead cells use sialic acid to maintain cell surface glycosylation through complementary mechanisms. First, levels of key metabolites (e.g., UDP-GlcNAc and CMP-Neu5Ac) required for glycan biosynthesis are maintained or enhanced upon Neu5Ac supplementation. In concert, sialyltransferase expression increased at both the mRNA and protein levels, which facilitated increased sialylation in biochemical assays that measure sialyltransferase activity as well as at the whole cell level. In the course of these experiments, several important differences emerged that differentiated the cancer cells from their normal counterparts including resistant to sialic acidmediated energy depletion, consistently more robust sialic acid-mediated glycan display, and distinctive cell surface vs. internal vesicle display of newly-produced sialoglycans. Finally, the impact of sialic acid supplementation on specific markers implicated in cancer progression was demonstrated by measuring levels of expression and sialylation of EGFR1 and MUC1 as well as the corresponding function of sialic acid-supplemented cells in migration assays. These findings both provide fundamental insight into the biological basis of sialic acid supplementation of nutrient-deprived cancer cells and open the door to the development of diagnostic and prognostic tools.

Research paper thumbnail of Harnessing cancer cell metabolism for theranostic applications using metabolic glycoengineering of sialic acid in breast cancer as a pioneering example

Biomaterials, 2017

Abnormal cell surface display of sialic acids-a family of unusual 9-carbon sugars-is widely recog... more Abnormal cell surface display of sialic acids-a family of unusual 9-carbon sugars-is widely recognized as distinguishing feature of many types of cancer. Sialoglycans, however, typically cannot be identified with sufficiently high reproducibility and sensitivity to serve as clinically accepted biomarkers and similarly, almost all efforts to exploit cancer-specific differences in sialylation signatures for therapy remain in early stage development. In this report we provide an overview of important facets of glycosylation that contribute to cancer in general with a focus on breast cancer as an example of malignant disease characterized by aberrant sialylation. We then describe how cancer cells experience nutrient deprivation during oncogenesis and discuss how the resulting metabolic reprogramming, which endows breast cancer cells with the ability to obtain nutrients during scarcity, constitutes an "Achilles' heel" that we believe can be exploited by metabolic glycoengineering (MGE) strategies to develop new diagnostic methods and therapeutic approaches. In particular, we hypothesize that adaptations made by breast cancer cells that allow them to efficiently scavenge sialic acid during times of nutrient deprivation renders them vulnerable to MGE, which refers to the use of exogenously-supplied, non-natural monosaccharide analogues to modulate targeted aspects of glycosylation in living cells and animals. In specific, once non-natural sialosides are incorporated into the cancer "sialome" they can be exploited as

Research paper thumbnail of Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats

Ecotoxicology and Environmental Safety

Nanotechnology is used in a wide range of applications, including medical therapies that precisel... more Nanotechnology is used in a wide range of applications, including medical therapies that precisely target disease prevention and treatment. The current study aimed firstly, to synthesize selenium nanoparticles (SeNPs) in an eco-friendly manner using Moringa oleifera leaf extract (MOLE). Secondly, to compare the protective effects of green-synthesized MOLE-SeNPs conjugate and MOLE ethanolic extract as remedies for melamine (MEL) induced nephrotoxicity in male rats. One hundred and five male Sprague Dawley rats were divided into seven groups (n = 15), including 1st control, 2nd MOLE (800 mg/kg BW), 3rd SeNPs (0.5 mg/kg BW), 4th MOLE-SeNPs (200 μg/kg BW), 5th MEL (700 mg/kg BW), 6th MEL+MOLE, and 7th MEL+MOLE SeNPs. All groups were orally gavaged day after day for 28 days. SeNPs and the colloidal SeNPs were characterized by TEM, SEM, and DLS particle size. SeNPs showed an absorption peak at a wavelength of 530 nm, spherical shape, and an average size between 3.2 and 20 nm. Colloidal SeNPs absorption spectra were recorded between 400 and 700 nm with an average size of 3.3-17 nm. MEL-induced nephropathic alterations represented by a significant increase in serum creatinine, urea, blood urea nitrogen (BUN), renal TNFα, oxidative stress-related indices, and altered the relative mRNA expression of apoptosis-related genes Bax, Caspase-3, Bcl2, Fas, and FasL. MEL-induced array of nephrotoxic morphological changes, and up-regulated immune-expression of proliferating cell nuclear antigen (PCNA) and proliferation-associated nuclear antigen Ki-67. Administration of MOLE or MOLE-SeNPs significantly reversed MEL-induced renal function impairments, oxidative stress, histological alterations, modulation in the relative mRNA expression of apoptosis-related genes, and the immune-expression of renal PCNA and Ki-67. Conclusively, the green-synthesized MOLE-SeNPs and MOLE display nephron-protective properties against MEL-induced murine nephropathy. This study is the first to report these effects which were more pronounced in the MOLE group than the green biosynthesized MOLE-SeNPs conjugate group.

Research paper thumbnail of Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats

Ecotoxicology and environmental safety, 2021

Nanotechnology is used in a wide range of applications, including medical therapies that precisel... more Nanotechnology is used in a wide range of applications, including medical therapies that precisely target disease prevention and treatment. The current study aimed firstly, to synthesize selenium nanoparticles (SeNPs) in an eco-friendly manner using Moringa oleifera leaf extract (MOLE). Secondly, to compare the protective effects of green-synthesized MOLE-SeNPs conjugate and MOLE ethanolic extract as remedies for melamine (MEL) induced nephrotoxicity in male rats. One hundred and five male Sprague Dawley rats were divided into seven groups (n = 15), including 1st control, 2nd MOLE (800 mg/kg BW), 3rd SeNPs (0.5 mg/kg BW), 4th MOLE-SeNPs (200 μg/kg BW), 5th MEL (700 mg/kg BW), 6th MEL+MOLE, and 7th MEL+MOLE SeNPs. All groups were orally gavaged day after day for 28 days. SeNPs and the colloidal SeNPs were characterized by TEM, SEM, and DLS particle size. SeNPs showed an absorption peak at a wavelength of 530 nm, spherical shape, and an average size between 3.2 and 20 nm. Colloidal Se...

Research paper thumbnail of Development of a Novel Metabolic Glyco-Sensing Approach with Anti-Aging Potential

Mansoura Veterinary Medical Journal, 2018

Senescent cells, characterized by irreversible cell cycle arrest, are known to accumulate during ... more Senescent cells, characterized by irreversible cell cycle arrest, are known to accumulate during aging and their accumulation in aged tissues, in turn, accelerates aging process and promotes age-related diseases. Therefore, targeting senescent cells is an attractive strategy for anti-aging or to delay features of aging. Here, we develop a glyco-sensing approach to specifically eliminate senescent cells using rat luminal ductal epithelial (LDE) breast milk cells as a model system. Senescent LDE cells, when treated with a metabolic sugar sialic acid (SA) under nutrient deprived condition, undergo ~3-fold (p<0.001) more apoptosis compared to LDE adult cells as demonstrated by TUNEL assay. On SA binding lectin assays, particularly with Maackia amurensis lectin I (MAL-I), senescent cells show more sialylation compared to the adult cells, possibly causing more apoptosis on these cells. This study is the first to show that SA supplementation can be protective to aging cells experiencing...

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Effect of Dietary Medium-Chain α-Monoglycerides on the Growth Performance, Intestinal Histomorphology, Amino Acid Digestibility, and Broiler Chickens’ Blood Biochemical Parameters

Simple Summary The addition of biologically active materials to animal feed is a very recent topi... more Simple Summary The addition of biologically active materials to animal feed is a very recent topic regarding antibiotic alternatives. This study inspected the influence of graded levels of medium-chain α-monoglycerides, glycerol monolaurate (GML) on the growth performance, apparent ileal digestibility coefficient (AID%) of amino acids, and intestinal histomorphology of broiler chickens. Broiler chickens (76.82 g ± 0.40, n = 200) were fed on four experimental diets that were complemented with 0; 1; 3; or 5 g kg−1 glycerol monolaurate (GML0; GML1; GML3; and GML5). The findings suggested that glycerol monolaurate supplementation can improve the immune status and intestinal histomorphology of broiler chickens with no improving effect on the growth performance. Abstract This trial was conducted to assess the impact of medium-chain α-monoglycerides, glycerol monolaurate (GML) supplementation on the growth performance, apparent ileal digestibility coefficient (AID%) of amino acids, intesti...

Research paper thumbnail of Pigeon and Fowl Hearts: A Comparative Morphological and Immunohistochemical (Troponin T) Study

Similar to mammals, the avian heart is a muscular pump responsible for distributing blood through... more Similar to mammals, the avian heart is a muscular pump responsible for distributing blood throughout the body. However, a larger relative heart weight to body weight (RHW/BW) was reported in birds than mammals. Furthermore, it has been revealed that smaller birds specially flying one have relatively larger hearts (relative to body mass) than larger birds (non-flying). Although this fact, there are no literatures regarding comparative descriptive variations of the heart in flying and non-flying birds that could adapt various functions, such as flying and running. Therefore, this study aimed to conduct a comparative anatomical features and morphometrical measurments between the hearts of pigeon and fowl. The present study also analyzed the immunohistochemical expression of Troponin T (TnT) as one of the major regulator of striated muscle contraction. The study revealed significant larger RHW/BW in pigeon heart. Furthermore, the hearts of both fowl and pigeon are directed caudoventrall...

Research paper thumbnail of Tissue Engineering in Wound Healing Therapy by Using Chitosan and Stem Cells

Zagazig Veterinary Journal

Skin wounds are considered as the most critical, and widespread problems due to the high rate of ... more Skin wounds are considered as the most critical, and widespread problems due to the high rate of accidents or burn worldwide. Full-thickness skin loss could be chronic or acute wounds, which found difficulty in medical treatment, because of taking a long time in healing. Therefore, exposure to the infection, which delays the healing process and causing disordered healing, ends with distortion or incomplete closure, which might lead to a fatal complication. Thus, the need for skin grafting is a necessary and critical decision although its side effects and rejection possibilities. This review put a spotlight on one of the latest therapeutic techniques in regenerative medicine "tissue engineering". It is an up-to-dates field in applications of stem cells and cells culture to replace the traditional tissue or organs transplantation techniques. We discussed the skin organ scaffolding as a simple example of tissue engineering applications in accelerating wound healing using stem cells cultured with chitosan scaffold. The latter is characterized by several medical properties especially in skin regeneration, for fabrication of artificial skin, which mimics the natural skin tissue and applying it in chronic or acute wounds therapy.

Research paper thumbnail of Cancer cell death induced by nanomagnetolectin

European journal of cell biology, Jan 10, 2017

Magnetic nanoparticles represent a new paradigm for molecular targeting therapy in cancer. Howeve... more Magnetic nanoparticles represent a new paradigm for molecular targeting therapy in cancer. However, the transformative targeting potential of magnetic nanoparticles has been stymied by a key obstacle-safe delivery to specified target cells in vivo. As cancer cells grow under nutrient deprivation and hypoxic conditions and decorate cell surface with excessive sialoglycans, sialic acid binding lectins might be suitable for targeting cancer cells in vivo. Here we explore the potential of magnetic nanoparticles functionalized with wheat germ lectin (WGA) conjugate, so-called nanomagnetolectin, as apoptotic targetable agents for prostate cancer. In the presence of magnetic field (magnetofection) for 15min, 2.46nM nanomagnetolectin significantly promoted apoptosis (∼12-fold, p value <0.01) of prostate cancer cells (LNCaP, PC-3, DU-145) compared to normal prostate epithelial cells (PrEC, PNT2, PZ-HPV-7), when supplemented with 10mM sialic acid under nutrient deprived condition. Nanomagn...

Research paper thumbnail of Lectin approaches for glycoproteomics in FDA-approved cancer biomarkers

Expert Review of Proteomics, 2014

•• Study on cancer-associated glycosylation using lectins and glycan-binding antibodies.

Research paper thumbnail of Galectin-3 as a Potential Target to Prevent Cancer Metastasis

Clinical Medicine Insights: Oncology, 2015

Interactions between two cells or between cell and extracellular matrix mediated by protein-carbo... more Interactions between two cells or between cell and extracellular matrix mediated by protein-carbohydrate interactions play pivotal roles in modulating various biological processes such as growth regulation, immune function, cancer metastasis, and apoptosis. Galectin-3, a member of the β-galactoside-binding lectin family, is involved in fibrosis as well as cancer progression and metastasis, but the detailed mechanisms of its functions remain elusive. This review discusses its structure, carbohydrate-binding properties, and involvement in various aspects of tumorigenesis and some potential carbohydrate ligands that are currently investigated to block galectin-3 activity.

Research paper thumbnail of Lectin staining and Western blot data showing differential sialylation of nutrient-deprived cancer cells to sialic acid supplementation

Data in Brief, 2015

This report provides data that are specifically related to the differential sialylation of nutrie... more This report provides data that are specifically related to the differential sialylation of nutrient deprived breast cancer cells to sialic acid supplementation in support of the research article entitled, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; [1]. Particularly, breast cancer cells, when supplemented with sialic acid under nutrient deprivation, display sialylated glycans at the cell surface, but non-malignant mammary cells show sialylated glycans intracellularly. The impact of sialic acid supplementation under nutrient deprivation was demonstrated by measuring levels of expression and sialylation of two markers, EGFR1 and MUC1. This Data in Brief article complements the main manuscript by providing detailed instructions and representative results for cell-level imaging and Western blot analyses of changes in sialylation during nutrient deprivation and sialic acid supplementation. These methods can be readily generalized for the study of many types of glycosylation and various glycoprotein markers through the appropriate selection of fluorescently-labeled lectins.

Research paper thumbnail of Optimizing the Growth, Health, Reproductive Performance, and Gonadal Histology of Broodstock Fantail Goldfish (Carassius auratus, L.) by Dietary Cacao Bean Meal

Animals, 2020

The potential effects of cacao bean meal, Theobroma cacao L., (CBM) on the growth, health status,... more The potential effects of cacao bean meal, Theobroma cacao L., (CBM) on the growth, health status, blood biochemical parameters, antioxidant, immune status, physiological parameters, female reproductive performance, and gonadal histological features of fantail goldfish (Carassius auratus, L.) were evaluated using a complete randomized block design with sex as a block. The trial lasted for 60 days. A total of 54 healthy fantail goldfish (36 broodstock females and 18 broodstock males) were randomly allocated into three treatments with supplementation of three levels of cocoa powder 0, 5, and 10 g kg−1 diet, CBM0, CBM5, and CBM10, respectively, with the sex ratio being four females:two males per replicate. The body weight gain and feed conversion ratio of males were increased in the CBM10 treatment (p < 0.05). The CBM10 diet improved relative feed costs (p < 0.05). Females fed on the CBM10 diet had an increase in the serum level of total protein (p = 0.001). Females fed on a diet ...

Research paper thumbnail of Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection

Zagazig Veterinary Journal

The ultimate goal of cancer science is to learn how to select a cancer cell surface. Glycans in c... more The ultimate goal of cancer science is to learn how to select a cancer cell surface. Glycans in cancer cells are frequently at varying quantities or have fundamentally various structures than those seen in normal cells. The presence of sialic acid (Neu5Ac), which is prevalent at the end of glycan chains, is significant. N-glycolylneuraminic acid (Neu5Gc) is a "non-human" sugar type that intercept into Neu5Ac natural biosynthetic machinery, presaging as a perspective biomarker. Malignant breast cells resistance to chemotherapy and their non-malignant counterparts, when treated with the Neu5Gc under nutrient deprivation, display cell surface tumor-associated Neu5Gc-rich glycans, which is capture with Neu5Gc linkage specific plant lectins such as Triticum vulgaris agglutinin (WGA), Sambucus nigra agglutinin (SNA), and Maackia amurensis agglutinin I (MAL-I). Nevertheless, MAL-I binding differentiates surface of malignant breast cell line resistance to chemotherapy MCF-7/H compared to normal mammary epithelial cell line MCF-10/A. These findings emphasize the importance of one oxygen atom in Neu5Gc; it's supplementing of nutrient-depleted cancer cells' resistance to chemotherapy and facilitates the way for the implementation of better diagnostic and prognostic approaches.

Research paper thumbnail of Infoguides BUS110 Euripides Home

Research paper thumbnail of An automated pipeline for studying binding affinity of SARS-CoV-2 variants and the human ACE2 receptor

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research, 2021

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research, 2021

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Liver-Specific Stem Cells and Hepatocellular Carcinoma: An in Vitro Comparison Between Stem Cells Isolated from Malignant and Non- Malignant Human Liver Tissue

Journal of the Medical Research Institute, 2016

Research paper thumbnail of Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation

Biomaterials, 2015

Cancer is characterized by abnormal energy metabolism shaped by nutrient deprivation that maligna... more Cancer is characterized by abnormal energy metabolism shaped by nutrient deprivation that malignant cells experience during various stages of tumor development. This study investigated the response of nutrient-deprived cancer cells and their non-malignant counterparts to sialic acid supplementation and found that cells utilize negligible amounts of this sugar for energy. Instead cells use sialic acid to maintain cell surface glycosylation through complementary mechanisms. First, levels of key metabolites (e.g., UDP-GlcNAc and CMP-Neu5Ac) required for glycan biosynthesis are maintained or enhanced upon Neu5Ac supplementation. In concert, sialyltransferase expression increased at both the mRNA and protein levels, which facilitated increased sialylation in biochemical assays that measure sialyltransferase activity as well as at the whole cell level. In the course of these experiments, several important differences emerged that differentiated the cancer cells from their normal counterparts including resistant to sialic acidmediated energy depletion, consistently more robust sialic acid-mediated glycan display, and distinctive cell surface vs. internal vesicle display of newly-produced sialoglycans. Finally, the impact of sialic acid supplementation on specific markers implicated in cancer progression was demonstrated by measuring levels of expression and sialylation of EGFR1 and MUC1 as well as the corresponding function of sialic acid-supplemented cells in migration assays. These findings both provide fundamental insight into the biological basis of sialic acid supplementation of nutrient-deprived cancer cells and open the door to the development of diagnostic and prognostic tools.

Research paper thumbnail of Harnessing cancer cell metabolism for theranostic applications using metabolic glycoengineering of sialic acid in breast cancer as a pioneering example

Biomaterials, 2017

Abnormal cell surface display of sialic acids-a family of unusual 9-carbon sugars-is widely recog... more Abnormal cell surface display of sialic acids-a family of unusual 9-carbon sugars-is widely recognized as distinguishing feature of many types of cancer. Sialoglycans, however, typically cannot be identified with sufficiently high reproducibility and sensitivity to serve as clinically accepted biomarkers and similarly, almost all efforts to exploit cancer-specific differences in sialylation signatures for therapy remain in early stage development. In this report we provide an overview of important facets of glycosylation that contribute to cancer in general with a focus on breast cancer as an example of malignant disease characterized by aberrant sialylation. We then describe how cancer cells experience nutrient deprivation during oncogenesis and discuss how the resulting metabolic reprogramming, which endows breast cancer cells with the ability to obtain nutrients during scarcity, constitutes an "Achilles' heel" that we believe can be exploited by metabolic glycoengineering (MGE) strategies to develop new diagnostic methods and therapeutic approaches. In particular, we hypothesize that adaptations made by breast cancer cells that allow them to efficiently scavenge sialic acid during times of nutrient deprivation renders them vulnerable to MGE, which refers to the use of exogenously-supplied, non-natural monosaccharide analogues to modulate targeted aspects of glycosylation in living cells and animals. In specific, once non-natural sialosides are incorporated into the cancer "sialome" they can be exploited as

Research paper thumbnail of Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats

Ecotoxicology and Environmental Safety

Nanotechnology is used in a wide range of applications, including medical therapies that precisel... more Nanotechnology is used in a wide range of applications, including medical therapies that precisely target disease prevention and treatment. The current study aimed firstly, to synthesize selenium nanoparticles (SeNPs) in an eco-friendly manner using Moringa oleifera leaf extract (MOLE). Secondly, to compare the protective effects of green-synthesized MOLE-SeNPs conjugate and MOLE ethanolic extract as remedies for melamine (MEL) induced nephrotoxicity in male rats. One hundred and five male Sprague Dawley rats were divided into seven groups (n = 15), including 1st control, 2nd MOLE (800 mg/kg BW), 3rd SeNPs (0.5 mg/kg BW), 4th MOLE-SeNPs (200 μg/kg BW), 5th MEL (700 mg/kg BW), 6th MEL+MOLE, and 7th MEL+MOLE SeNPs. All groups were orally gavaged day after day for 28 days. SeNPs and the colloidal SeNPs were characterized by TEM, SEM, and DLS particle size. SeNPs showed an absorption peak at a wavelength of 530 nm, spherical shape, and an average size between 3.2 and 20 nm. Colloidal SeNPs absorption spectra were recorded between 400 and 700 nm with an average size of 3.3-17 nm. MEL-induced nephropathic alterations represented by a significant increase in serum creatinine, urea, blood urea nitrogen (BUN), renal TNFα, oxidative stress-related indices, and altered the relative mRNA expression of apoptosis-related genes Bax, Caspase-3, Bcl2, Fas, and FasL. MEL-induced array of nephrotoxic morphological changes, and up-regulated immune-expression of proliferating cell nuclear antigen (PCNA) and proliferation-associated nuclear antigen Ki-67. Administration of MOLE or MOLE-SeNPs significantly reversed MEL-induced renal function impairments, oxidative stress, histological alterations, modulation in the relative mRNA expression of apoptosis-related genes, and the immune-expression of renal PCNA and Ki-67. Conclusively, the green-synthesized MOLE-SeNPs and MOLE display nephron-protective properties against MEL-induced murine nephropathy. This study is the first to report these effects which were more pronounced in the MOLE group than the green biosynthesized MOLE-SeNPs conjugate group.

Research paper thumbnail of Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats

Ecotoxicology and environmental safety, 2021

Nanotechnology is used in a wide range of applications, including medical therapies that precisel... more Nanotechnology is used in a wide range of applications, including medical therapies that precisely target disease prevention and treatment. The current study aimed firstly, to synthesize selenium nanoparticles (SeNPs) in an eco-friendly manner using Moringa oleifera leaf extract (MOLE). Secondly, to compare the protective effects of green-synthesized MOLE-SeNPs conjugate and MOLE ethanolic extract as remedies for melamine (MEL) induced nephrotoxicity in male rats. One hundred and five male Sprague Dawley rats were divided into seven groups (n = 15), including 1st control, 2nd MOLE (800 mg/kg BW), 3rd SeNPs (0.5 mg/kg BW), 4th MOLE-SeNPs (200 μg/kg BW), 5th MEL (700 mg/kg BW), 6th MEL+MOLE, and 7th MEL+MOLE SeNPs. All groups were orally gavaged day after day for 28 days. SeNPs and the colloidal SeNPs were characterized by TEM, SEM, and DLS particle size. SeNPs showed an absorption peak at a wavelength of 530 nm, spherical shape, and an average size between 3.2 and 20 nm. Colloidal Se...

Research paper thumbnail of Development of a Novel Metabolic Glyco-Sensing Approach with Anti-Aging Potential

Mansoura Veterinary Medical Journal, 2018

Senescent cells, characterized by irreversible cell cycle arrest, are known to accumulate during ... more Senescent cells, characterized by irreversible cell cycle arrest, are known to accumulate during aging and their accumulation in aged tissues, in turn, accelerates aging process and promotes age-related diseases. Therefore, targeting senescent cells is an attractive strategy for anti-aging or to delay features of aging. Here, we develop a glyco-sensing approach to specifically eliminate senescent cells using rat luminal ductal epithelial (LDE) breast milk cells as a model system. Senescent LDE cells, when treated with a metabolic sugar sialic acid (SA) under nutrient deprived condition, undergo ~3-fold (p<0.001) more apoptosis compared to LDE adult cells as demonstrated by TUNEL assay. On SA binding lectin assays, particularly with Maackia amurensis lectin I (MAL-I), senescent cells show more sialylation compared to the adult cells, possibly causing more apoptosis on these cells. This study is the first to show that SA supplementation can be protective to aging cells experiencing...

Research paper thumbnail of Effect of supplemental glycerol monolaurate and oregano essential oil blend on the growth performance, intestinal morphology, and amino acid digestibility of broiler chickens

BMC Veterinary Research

Background This experiment tested the impact of the combined supplementation of glycerol monolaur... more Background This experiment tested the impact of the combined supplementation of glycerol monolaurate (GLM) and oregano essential oil (EO) to broiler diets. Growth performance, metabolic response, immune status, apparent ileal digestibility coefficient (AID%), and intestinal histomorphology were assessed. Three-day-old Ross-308 broilers (76.62 g ± 0.50, n = 240) were randomly allocated into 4 experimental groups (6 replicates/group and 10 chicks/replicate). Birds were fed corn-soybean meal basal diets supplemented with four levels of GLM and oregano EO blend: 0, 0.15, 0.45, and 0.75% for 35 days. Results During the starter period, dietary GLM and oregano EO did not show significant (P > 0.05) changes in growth performance. During the grower period, GLM and oregano EO supplemented groups showed a linear and quadratic decline in FCR. During the finisher and overall performance, a linear increase in the body weight (BW), body weight gain (BWG), the protein efficiency ratio (PER), and...

Research paper thumbnail of Effect of Dietary Medium-Chain α-Monoglycerides on the Growth Performance, Intestinal Histomorphology, Amino Acid Digestibility, and Broiler Chickens’ Blood Biochemical Parameters

Simple Summary The addition of biologically active materials to animal feed is a very recent topi... more Simple Summary The addition of biologically active materials to animal feed is a very recent topic regarding antibiotic alternatives. This study inspected the influence of graded levels of medium-chain α-monoglycerides, glycerol monolaurate (GML) on the growth performance, apparent ileal digestibility coefficient (AID%) of amino acids, and intestinal histomorphology of broiler chickens. Broiler chickens (76.82 g ± 0.40, n = 200) were fed on four experimental diets that were complemented with 0; 1; 3; or 5 g kg−1 glycerol monolaurate (GML0; GML1; GML3; and GML5). The findings suggested that glycerol monolaurate supplementation can improve the immune status and intestinal histomorphology of broiler chickens with no improving effect on the growth performance. Abstract This trial was conducted to assess the impact of medium-chain α-monoglycerides, glycerol monolaurate (GML) supplementation on the growth performance, apparent ileal digestibility coefficient (AID%) of amino acids, intesti...

Research paper thumbnail of Pigeon and Fowl Hearts: A Comparative Morphological and Immunohistochemical (Troponin T) Study

Similar to mammals, the avian heart is a muscular pump responsible for distributing blood through... more Similar to mammals, the avian heart is a muscular pump responsible for distributing blood throughout the body. However, a larger relative heart weight to body weight (RHW/BW) was reported in birds than mammals. Furthermore, it has been revealed that smaller birds specially flying one have relatively larger hearts (relative to body mass) than larger birds (non-flying). Although this fact, there are no literatures regarding comparative descriptive variations of the heart in flying and non-flying birds that could adapt various functions, such as flying and running. Therefore, this study aimed to conduct a comparative anatomical features and morphometrical measurments between the hearts of pigeon and fowl. The present study also analyzed the immunohistochemical expression of Troponin T (TnT) as one of the major regulator of striated muscle contraction. The study revealed significant larger RHW/BW in pigeon heart. Furthermore, the hearts of both fowl and pigeon are directed caudoventrall...

Research paper thumbnail of Tissue Engineering in Wound Healing Therapy by Using Chitosan and Stem Cells

Zagazig Veterinary Journal

Skin wounds are considered as the most critical, and widespread problems due to the high rate of ... more Skin wounds are considered as the most critical, and widespread problems due to the high rate of accidents or burn worldwide. Full-thickness skin loss could be chronic or acute wounds, which found difficulty in medical treatment, because of taking a long time in healing. Therefore, exposure to the infection, which delays the healing process and causing disordered healing, ends with distortion or incomplete closure, which might lead to a fatal complication. Thus, the need for skin grafting is a necessary and critical decision although its side effects and rejection possibilities. This review put a spotlight on one of the latest therapeutic techniques in regenerative medicine "tissue engineering". It is an up-to-dates field in applications of stem cells and cells culture to replace the traditional tissue or organs transplantation techniques. We discussed the skin organ scaffolding as a simple example of tissue engineering applications in accelerating wound healing using stem cells cultured with chitosan scaffold. The latter is characterized by several medical properties especially in skin regeneration, for fabrication of artificial skin, which mimics the natural skin tissue and applying it in chronic or acute wounds therapy.

Research paper thumbnail of Cancer cell death induced by nanomagnetolectin

European journal of cell biology, Jan 10, 2017

Magnetic nanoparticles represent a new paradigm for molecular targeting therapy in cancer. Howeve... more Magnetic nanoparticles represent a new paradigm for molecular targeting therapy in cancer. However, the transformative targeting potential of magnetic nanoparticles has been stymied by a key obstacle-safe delivery to specified target cells in vivo. As cancer cells grow under nutrient deprivation and hypoxic conditions and decorate cell surface with excessive sialoglycans, sialic acid binding lectins might be suitable for targeting cancer cells in vivo. Here we explore the potential of magnetic nanoparticles functionalized with wheat germ lectin (WGA) conjugate, so-called nanomagnetolectin, as apoptotic targetable agents for prostate cancer. In the presence of magnetic field (magnetofection) for 15min, 2.46nM nanomagnetolectin significantly promoted apoptosis (∼12-fold, p value <0.01) of prostate cancer cells (LNCaP, PC-3, DU-145) compared to normal prostate epithelial cells (PrEC, PNT2, PZ-HPV-7), when supplemented with 10mM sialic acid under nutrient deprived condition. Nanomagn...

Research paper thumbnail of Lectin approaches for glycoproteomics in FDA-approved cancer biomarkers

Expert Review of Proteomics, 2014

•• Study on cancer-associated glycosylation using lectins and glycan-binding antibodies.

Research paper thumbnail of Galectin-3 as a Potential Target to Prevent Cancer Metastasis

Clinical Medicine Insights: Oncology, 2015

Interactions between two cells or between cell and extracellular matrix mediated by protein-carbo... more Interactions between two cells or between cell and extracellular matrix mediated by protein-carbohydrate interactions play pivotal roles in modulating various biological processes such as growth regulation, immune function, cancer metastasis, and apoptosis. Galectin-3, a member of the β-galactoside-binding lectin family, is involved in fibrosis as well as cancer progression and metastasis, but the detailed mechanisms of its functions remain elusive. This review discusses its structure, carbohydrate-binding properties, and involvement in various aspects of tumorigenesis and some potential carbohydrate ligands that are currently investigated to block galectin-3 activity.

Research paper thumbnail of Lectin staining and Western blot data showing differential sialylation of nutrient-deprived cancer cells to sialic acid supplementation

Data in Brief, 2015

This report provides data that are specifically related to the differential sialylation of nutrie... more This report provides data that are specifically related to the differential sialylation of nutrient deprived breast cancer cells to sialic acid supplementation in support of the research article entitled, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; [1]. Particularly, breast cancer cells, when supplemented with sialic acid under nutrient deprivation, display sialylated glycans at the cell surface, but non-malignant mammary cells show sialylated glycans intracellularly. The impact of sialic acid supplementation under nutrient deprivation was demonstrated by measuring levels of expression and sialylation of two markers, EGFR1 and MUC1. This Data in Brief article complements the main manuscript by providing detailed instructions and representative results for cell-level imaging and Western blot analyses of changes in sialylation during nutrient deprivation and sialic acid supplementation. These methods can be readily generalized for the study of many types of glycosylation and various glycoprotein markers through the appropriate selection of fluorescently-labeled lectins.