Mohamed Nassan | Zagazig University (original) (raw)
Papers by Mohamed Nassan
American Journal of Infectious Diseases, 2016
National Journal of Physiology, Pharmacy and Pharmacology, 2016
Experimental and Therapeutic Medicine, 2015
Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized... more Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized by insulin resistance (IR), which leads to dyslipidemia and alterations in the expression levels of a number of genes. Metformin is the standard treatment for T2DM; however, the exact mechanism underlying metformin regulation is not fully understood. The aim of the present study was to investigate the effects of metformin on serum lipid profiles and the expression levels of various genes that are associated with IR, as well as the histopathological changes in the liver and pancreas. A T2DM rat model was established by feeding the rats a high-fat diet for 4 weeks, combined with a dose of streptozotocin (35 mg/kg body weight). Following the successful induction of T2DM, metformin was administered orally (400 mg/kg/day) for 4 weeks. The results indicated that metformin improved the symptoms of IR by normalizing the serum lipid profiles in the diabetic rats. Furthermore, metformin upregulated the expression of insulin receptors and genes associated with lipid metabolism, including acyl-CoA oxidase, carnitine palmitoyl transferase-1 and peroxisome proliferator activated receptor-α. In addition, treatment with metformin downregulated the expression levels of fetuin-A and retinol binding protein-4 (RBP-4), while normalizing the expression of perilipin that had been reduced in the T2DM rats. Metformin administration induced regenerative changes in the hepatocyte cytoplasm and parenchyma. In the pancreas, treatment with metformin was shown to induce positive signaling for insulin and the regeneration of pancreatic β cells. In summary, metformin treatment ameliorated a number of the harmful effects associated with T2DM via the modulation of the expression levels of fetuin-A, RBP-4, perilipin and various genes associated with lipid metabolism, resulting in regenerative changes in the liver and pancreatic cells.
Journal of Cytology & Histology, 2013
ABSTRACT The present study was undertaken to correlate the immunohistochemical expression of Bcl-... more ABSTRACT The present study was undertaken to correlate the immunohistochemical expression of Bcl-2 oncoprotein in buffalo’s adrenal gland in the young and old ages. Paraffin-embedded sections from the adrenal glands representing the young and old ages were immunostained for Bcl-2 oncoprotein. A higher percentage of Bcl-2 immunoreactivity was detected in the adrenal cortical cells of the buffaloes at young ages. The immunoreactivity was moderate in the adrenal cortical cells of the old ages. The degree of intensity was prominent in the zona reticularis than that of the other zones, while negative immunoreactivity in the cells of the adrenal medulla. In conclusion, the immunohistochemical expression of Bcl-2 oncoprotein in the young buffalo’s adrenal gland (antiapoptotic gene) was prominent than the old ages. So the incidence of apoptosis was prominent in the adrenal gland cortical cells of the old age than that the adrenal gland cortical cells of the young ages.
International journal of immunopathology and pharmacology, 2015
Recent studies showed prominent antimicrobial activity of some plant extracts on some pathogenic ... more Recent studies showed prominent antimicrobial activity of some plant extracts on some pathogenic microorganisms so we evaluated antimicrobial activity of aqueous extracts of clove and cinnamon using the agar well diffusion method. An in vivo study was carried out on 40 adult healthy male albino rats divided into four groups: Group 1: negative control group (received intragastric saline solution daily); Group 2: injected with mixed bacterial suspension of S. aureus and E.coli as a model of pyelonephritis then received intragastric saline solution daily; Group 3: injected with the same dose of mixed bacterial suspension then received intragastric clove extract 500 mg/kg/day; and Group (4): injected with mixed bacterial suspension then received intragastric cinnamon 500 mg/kg/day. Five rats from each group were sacrificed after 1 and 4 weeks. Serum and blood samples were collected for lysozymes activity and nitric oxide production, lymphocyte transformation test, as well as counting of...
Experimental and Therapeutic Medicine, 2015
Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized... more Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized by insulin resistance (IR), which leads to dyslipidemia and alterations in the expression levels of a number of genes. Metformin is the standard treatment for T2DM; however, the exact mechanism underlying metformin regulation is not fully understood. The aim of the present study was to investigate the effects of metformin on serum lipid profiles and the expression levels of various genes that are associated with IR, as well as the histopathological changes in the liver and pancreas. A T2DM rat model was established by feeding the rats a high-fat diet for 4 weeks, combined with a dose of streptozotocin (35 mg/kg body weight). Following the successful induction of T2DM, metformin was administered orally (400 mg/kg/day) for 4 weeks. The results indicated that metformin improved the symptoms of IR by normalizing the serum lipid profiles in the diabetic rats. Furthermore, metformin upregulated the expression of insulin receptors and genes associated with lipid metabolism, including acyl-CoA oxidase, carnitine palmitoyl transferase-1 and peroxisome proliferator activated receptor-α. In addition, treatment with metformin downregulated the expression levels of fetuin-A and retinol binding protein-4 (RBP-4), while normalizing the expression of perilipin that had been reduced in the T2DM rats. Metformin administration induced regenerative changes in the hepatocyte cytoplasm and parenchyma. In the pancreas, treatment with metformin was shown to induce positive signaling for insulin and the regeneration of pancreatic β cells. In summary, metformin treatment ameliorated a number of the harmful effects associated with T2DM via the modulation of the expression levels of fetuin-A, RBP-4, perilipin and various genes associated with lipid metabolism, resulting in regenerative changes in the liver and pancreatic cells.
The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from on... more The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immunostaining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subunits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the inhibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.
Journal of Food and Nutrition Research, 2015
Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidenc... more Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidence of atherosclerosis and coronary artery diseases. Therefore, the present study was conducted to evaluate the hypolipidemic effect of widely known traditional medicinal herbs and omega-3 FA in experimental hypercholesterolemia induced by Triton WR-1339. Experimental hypercholesterolemic rats were administered mushroom, chrysin, curcumin and omega-3 for 2 weeks. Hypercholesterolemic rats showed an increase in serum levels of lipid profiles and hepatic enzymes. Hypercholesterolemic rats showed an increase in malondialdehyde (MDA) levels and a decrease in both serum levels and mRNA expression of catalase, superoxide dismutase (SOD) and glutathione reducatse. Moreover, hypercholesterolemic rats showed hepatic down regulation in the expression of genes related to fatty acids oxidation such as acyl CoA oxidase (ACO) and synthetase (ACS), together with carnityl palmityl transferase-1 (CPT-1) and peroxisome proliferator activator receptor-α (PPAR-α). Administration of mushroom, chrysin, curcumin and omega-3 to hypercholesterolemic rats for 2 weeks up-regulated significantly the down regulated genes. In contrast, expression of genes related to fatty acids biosynthesis and cholesterol metabolism were increased in hypercholesterolemic rats compared to control group. Herbal medications and omega-3 administration down regulated genes of fatty acids biosynthesis and cholesterol metabolism to normal expression. At cellular levels, hyperlipidemia induced fatty droplets accumulation, necrosis and presence of apoptotic hepatocytes together with leukocytic infiltration in necrotic area that are ameliorated and normalized after administration of herbs and omega-3. In conclusion, the current findings indicated that flavonoids (mushroom, chrysin, curcumin) and omega-3 possess antihypercholesterolemic effects at biochemical, molecular and histopathological levels and are useful in treatment of hypercholesterolemia with lower side effects compared with synthetic hypolipidemic drugs.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2014
Objective. The objective of this study was the evaluation of effects of locally administered zole... more Objective. The objective of this study was the evaluation of effects of locally administered zoledronic acid and alendronate on rate of osteogenesis in distracted mandible of dogs. Study Design. Following mandibular corticotomy, bone segments were maintained in a neutral position by distractor for 7 days then distraction was initiated at a rate of 0.5 mm twice a day for 10 days to achieve a total distraction of 10 mm, followed by a consolidation period. Animals were divided into 3 equal groups according to the injected drug (saline solution [control], zoledronic acid, alendronate). The dogs were killed 2, 6, and 10 weeks following distraction and samples were collected for radiographic examination, assessment of bone mineral density, and histopathological evaluation. Results. Radiographs and histopathological results pointed out that bone formation and maturation of experimental groups were faster than those of the control group. Conclusions. Local administration of zoledronic acid and alendronate proved to be effective in shortening the consolidation
BMC Complementary and Alternative Medicine, 2014
Background: An overdose of paracetamol is a frequent reason for liver and renal toxicity and poss... more Background: An overdose of paracetamol is a frequent reason for liver and renal toxicity and possible death and curcumin has hepatoprotective properties against liver damage. The exact mechanism of such protection is not clear. Therefore, this study was conducted to examine the molecular levels of the protective effect of curcumin on paracetamol overdose induced hepatic toxicity in rats.
International journal of immunopathology and pharmacology
Recent studies showed prominent antimicrobial activity of various plant extracts on certain patho... more Recent studies showed prominent antimicrobial activity of various plant extracts on certain pathogenic microorganisms, therefore we prepared crude aqueous extracts of black pepper, ginger and thyme and carried out an in vitro study by measuring antimicrobial activity of these extracts using the agar well diffusion method. An in vivo study was carried out on 50 adult healthy male albino rats which were divided into 5 groups, 10 rats each. Group 1: negative control group which received saline solution intragastrically daily; Group 2: Positive control group, injected with mixed bacterial suspension of S.aureus and E.coli as a model of pyelonephritis, then received saline solution intragastrically daily; Group 3: injected with the same dose of mixed bacterial suspension, then received 100 mg/kg/day black pepper extract intragastrically; Group 4: injected with mixed bacterial suspension then received 500 mg/kg/day ginger extract intragastrically. Group 5: injected with mixed bacterial su...
American Journal of Pharmacology and Toxicology, 2013
The present study was directed to evaluate the toxic effects of orally administered titanium diox... more The present study was directed to evaluate the toxic effects of orally administered titanium dioxide naonparticles (TiO2) on liver of male albino rats and to evaluate the ameliorative effects of N-acetylcysteine (NAC). Forty adult male albino rats were divided into 4 groups; control group, NAC group, TiO2 group and TiO2/ NAC group. Rats were administered either TiO2 (1200 mg kg −1 BW) or NAC (100 mg kg −1 BW) alone or together for 9 months. Blood was taken to evaluate serum changes in GPT, GOT and MDA levels. Liver tissues were examined for changes in MDA, GSH and changes in liver histopathology. Administration of TiO2 increased serum GPT, GOT and decreased MDA levels. Co-treatment of rats with NAC and TiO2 improved such significant changes induced by TiO2 alone. Moreover, significant time dependent increase in MDA and decrease in GSH levels in liver tissues were recorded. Liver histopathology showed vacuolar, hydropic degeneration and cell death of some hepatic cells. In conclusion, results confirmed the protective effect of NAC in amelioration of the biohazard effects induced by TiO2 in rats.
American Journal of Pharmacology and Toxicology, 2014
In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dio... more In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dioxide (TiO2) induced testicular degeneration in albino rats. Adult male albino rats were given saline as a control group, TiO2 (1200 mg kg −1 BW), NAC (100 mg kg −1 BW) and co-treatment of NAC and TiO2 as a protective group for 3 months. Testicular tissues were extracted for changes in testicular gene expression and histopathology. Administration of TiO2 significantly up-regulated mRNA expression of IL-6 and TNF-α and normalized by NAC administration. TiO2 administration down regulated Glutathione-S-Transferase (GST) while increased B-cell Lymphoma2 (BcL2) expressions. Co-administration of rats by NAC together with TiO2 normalized changes in GST and BcL2 expression. Expression of steroidogenesis related genes [Androgen Binding Protein (ABR), 17β-Hydroxysteroid Dehydrogenase (17β-HSD), cytochrome P450 17A (CYP17α) and aromatase] showed down regulation in TiO2 administered groups and normalized when NAC given together with TiO2. Moreover, TiO2 induced toxicity in testes that accompanied by degeneration in seminiferous tubules with congestion, oedema and cell disruption that are partially normalized by co-administration of NAC with TiO2. In conclusion, the present findings confirmed the benficial effect of NAC to prevent apoptosis in spermatogenic and sertoli cells and testicular dysfunction induced by TiO2 in male albino rats.
Background: Stevioside has been used as a medication for reducing glucose levels in diabetic pati... more Background: Stevioside has been used as a medication for reducing glucose levels in diabetic patients. The exact mode of action is
still unclear. Therefore, the current study outlined the molecular and biological roles of stevioside in treatment of diabetes.
Materials and Methods: Diabetic male Wistar rats were treated with stevioside and metformin as a medication for diabetes rats for
4 weeks. Biochemical, molecular and histopathological studies have been done to evaluate the therapeutic effect of stevioside on
minimizing levels of glucose and its related gene expression in diabetic rats.
Results: Stevioside administration normalized kidney and liver biomarkers, restored alterations in antioxidants activity and lipid
profiles. Moreover, stevioside increased insulin and leptin secretion that are decreased in diabetic rats to the normal levels. For
mRNA expression, stevioside up-regulated the expressions of PK and IRS-1 genes, which are down-regulated in diabetic rats, and
was very effective in the down-regulation of CPT-1 mRNA expression. At the cellular levels; stevioside normalized the
histopathological changes induced in pancreas.
Conclusion: Stevioside has insulin like effects and is useful for diabetic patient’s therapy.
Abstract. The present study aimed to investigate the molecular mechanism underlying the hepatopro... more Abstract. The present study aimed to investigate the molecular mechanism underlying the hepatoprotective effects of pomegranate (POM) against oxidative stress in a rat model of carbon tetrachloride (CCl4)‑induced liver damage. Injection of rats with CCl4 resulted in hepatic inflammation and lipid accumulation via the upregulation of interleukin (IL)‑6 and sterol regulatory element‑binding protein 1c (SREBP‑1c) mRNA expression. CCl4 induced downregulation of the anti‑inflammatory factors alpha 2‑macroglobulin (α‑2M) and IL‑10 in comparison with the POM treated group. In addition, CCl4 induced downregulation of superoxide dismutase (SOD), glutathione S‑transferase (GST) and catalase (CAT) expression. Conversely, prior administration of POM counteracted the deleterious alterations induced by CCl4. POM downregulated CCl4‑induced IL‑6 upregulation, normalized the increase in SREBP‑1c expression, and prevented CCl4‑induced α‑2M downregulation. POM counteracted CCL4‑induced alterations via immunosuppressive, anti‑inflammatory and regenerative effects by upregulating transforming growth factor‑β1, HSP70 and IL‑10 mRNA expression. In addition, POM increased reactive oxygen species scavenging activityby augmenting the antioxidant defense mechanism against CCl4 hepatotoxicity, as demonstrated by detecting SOD, CAT and GST expression. These results confirm that, at the molecular level, POM exerts hepatoprotective effects against CCl4‑induced oxidative stress and liver tissue damage.
The present study was conducted to test the effect of Origanum Majoranum Extract (OME) of leaves ... more The present study was conducted to test the effect of Origanum Majoranum Extract (OME)
of leaves on alterations induced in a model of type 2 diabetic rats. Adult male Wistar rats
were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg)
intraperitoneally to induce type 2 diabetic rats. Diabetic rats were given aqueous extract of
OME in a dose of 20 mg/kg orally for 3 weeks. Changes in lipid profiles, glucose, insulin,
expression of some genes related to glucose metabolism and histopathological changes in liver
and kidney were examined. Administration of OME improved and normalized dyslipidemia
recorded in type 2 diabetic rats together with reduction in glucose and insulin levels. OME
induced up-regulation in gene expression of glucose [adiponectin and glucose transporter-2
(GLUT-2)] and lipid metabolism [lipoprotein lipase (LPL)]. Moreover, OME normalized
histopathological changes occurred in liver and kidney of diabetic rats. OME decreased lipids
accumulation in liver and kidney and increased regeneration of hepatic parenchyma and
restored normal renal architecture with disappearance of fat droplets. In conclusion, OME
improved dyslipidemia associated with type 2 diabetes through regulation of genes related to
glucose and lipid metabolism.
American Journal of Infectious Diseases, 2016
National Journal of Physiology, Pharmacy and Pharmacology, 2016
Experimental and Therapeutic Medicine, 2015
Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized... more Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized by insulin resistance (IR), which leads to dyslipidemia and alterations in the expression levels of a number of genes. Metformin is the standard treatment for T2DM; however, the exact mechanism underlying metformin regulation is not fully understood. The aim of the present study was to investigate the effects of metformin on serum lipid profiles and the expression levels of various genes that are associated with IR, as well as the histopathological changes in the liver and pancreas. A T2DM rat model was established by feeding the rats a high-fat diet for 4 weeks, combined with a dose of streptozotocin (35 mg/kg body weight). Following the successful induction of T2DM, metformin was administered orally (400 mg/kg/day) for 4 weeks. The results indicated that metformin improved the symptoms of IR by normalizing the serum lipid profiles in the diabetic rats. Furthermore, metformin upregulated the expression of insulin receptors and genes associated with lipid metabolism, including acyl-CoA oxidase, carnitine palmitoyl transferase-1 and peroxisome proliferator activated receptor-α. In addition, treatment with metformin downregulated the expression levels of fetuin-A and retinol binding protein-4 (RBP-4), while normalizing the expression of perilipin that had been reduced in the T2DM rats. Metformin administration induced regenerative changes in the hepatocyte cytoplasm and parenchyma. In the pancreas, treatment with metformin was shown to induce positive signaling for insulin and the regeneration of pancreatic β cells. In summary, metformin treatment ameliorated a number of the harmful effects associated with T2DM via the modulation of the expression levels of fetuin-A, RBP-4, perilipin and various genes associated with lipid metabolism, resulting in regenerative changes in the liver and pancreatic cells.
Journal of Cytology & Histology, 2013
ABSTRACT The present study was undertaken to correlate the immunohistochemical expression of Bcl-... more ABSTRACT The present study was undertaken to correlate the immunohistochemical expression of Bcl-2 oncoprotein in buffalo’s adrenal gland in the young and old ages. Paraffin-embedded sections from the adrenal glands representing the young and old ages were immunostained for Bcl-2 oncoprotein. A higher percentage of Bcl-2 immunoreactivity was detected in the adrenal cortical cells of the buffaloes at young ages. The immunoreactivity was moderate in the adrenal cortical cells of the old ages. The degree of intensity was prominent in the zona reticularis than that of the other zones, while negative immunoreactivity in the cells of the adrenal medulla. In conclusion, the immunohistochemical expression of Bcl-2 oncoprotein in the young buffalo’s adrenal gland (antiapoptotic gene) was prominent than the old ages. So the incidence of apoptosis was prominent in the adrenal gland cortical cells of the old age than that the adrenal gland cortical cells of the young ages.
International journal of immunopathology and pharmacology, 2015
Recent studies showed prominent antimicrobial activity of some plant extracts on some pathogenic ... more Recent studies showed prominent antimicrobial activity of some plant extracts on some pathogenic microorganisms so we evaluated antimicrobial activity of aqueous extracts of clove and cinnamon using the agar well diffusion method. An in vivo study was carried out on 40 adult healthy male albino rats divided into four groups: Group 1: negative control group (received intragastric saline solution daily); Group 2: injected with mixed bacterial suspension of S. aureus and E.coli as a model of pyelonephritis then received intragastric saline solution daily; Group 3: injected with the same dose of mixed bacterial suspension then received intragastric clove extract 500 mg/kg/day; and Group (4): injected with mixed bacterial suspension then received intragastric cinnamon 500 mg/kg/day. Five rats from each group were sacrificed after 1 and 4 weeks. Serum and blood samples were collected for lysozymes activity and nitric oxide production, lymphocyte transformation test, as well as counting of...
Experimental and Therapeutic Medicine, 2015
Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized... more Type 2 diabetes mellitus (T2DM) is a serious health issue worldwide. The disease is characterized by insulin resistance (IR), which leads to dyslipidemia and alterations in the expression levels of a number of genes. Metformin is the standard treatment for T2DM; however, the exact mechanism underlying metformin regulation is not fully understood. The aim of the present study was to investigate the effects of metformin on serum lipid profiles and the expression levels of various genes that are associated with IR, as well as the histopathological changes in the liver and pancreas. A T2DM rat model was established by feeding the rats a high-fat diet for 4 weeks, combined with a dose of streptozotocin (35 mg/kg body weight). Following the successful induction of T2DM, metformin was administered orally (400 mg/kg/day) for 4 weeks. The results indicated that metformin improved the symptoms of IR by normalizing the serum lipid profiles in the diabetic rats. Furthermore, metformin upregulated the expression of insulin receptors and genes associated with lipid metabolism, including acyl-CoA oxidase, carnitine palmitoyl transferase-1 and peroxisome proliferator activated receptor-α. In addition, treatment with metformin downregulated the expression levels of fetuin-A and retinol binding protein-4 (RBP-4), while normalizing the expression of perilipin that had been reduced in the T2DM rats. Metformin administration induced regenerative changes in the hepatocyte cytoplasm and parenchyma. In the pancreas, treatment with metformin was shown to induce positive signaling for insulin and the regeneration of pancreatic β cells. In summary, metformin treatment ameliorated a number of the harmful effects associated with T2DM via the modulation of the expression levels of fetuin-A, RBP-4, perilipin and various genes associated with lipid metabolism, resulting in regenerative changes in the liver and pancreatic cells.
The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from on... more The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immunostaining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subunits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the inhibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.
Journal of Food and Nutrition Research, 2015
Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidenc... more Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidence of atherosclerosis and coronary artery diseases. Therefore, the present study was conducted to evaluate the hypolipidemic effect of widely known traditional medicinal herbs and omega-3 FA in experimental hypercholesterolemia induced by Triton WR-1339. Experimental hypercholesterolemic rats were administered mushroom, chrysin, curcumin and omega-3 for 2 weeks. Hypercholesterolemic rats showed an increase in serum levels of lipid profiles and hepatic enzymes. Hypercholesterolemic rats showed an increase in malondialdehyde (MDA) levels and a decrease in both serum levels and mRNA expression of catalase, superoxide dismutase (SOD) and glutathione reducatse. Moreover, hypercholesterolemic rats showed hepatic down regulation in the expression of genes related to fatty acids oxidation such as acyl CoA oxidase (ACO) and synthetase (ACS), together with carnityl palmityl transferase-1 (CPT-1) and peroxisome proliferator activator receptor-α (PPAR-α). Administration of mushroom, chrysin, curcumin and omega-3 to hypercholesterolemic rats for 2 weeks up-regulated significantly the down regulated genes. In contrast, expression of genes related to fatty acids biosynthesis and cholesterol metabolism were increased in hypercholesterolemic rats compared to control group. Herbal medications and omega-3 administration down regulated genes of fatty acids biosynthesis and cholesterol metabolism to normal expression. At cellular levels, hyperlipidemia induced fatty droplets accumulation, necrosis and presence of apoptotic hepatocytes together with leukocytic infiltration in necrotic area that are ameliorated and normalized after administration of herbs and omega-3. In conclusion, the current findings indicated that flavonoids (mushroom, chrysin, curcumin) and omega-3 possess antihypercholesterolemic effects at biochemical, molecular and histopathological levels and are useful in treatment of hypercholesterolemia with lower side effects compared with synthetic hypolipidemic drugs.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2014
Objective. The objective of this study was the evaluation of effects of locally administered zole... more Objective. The objective of this study was the evaluation of effects of locally administered zoledronic acid and alendronate on rate of osteogenesis in distracted mandible of dogs. Study Design. Following mandibular corticotomy, bone segments were maintained in a neutral position by distractor for 7 days then distraction was initiated at a rate of 0.5 mm twice a day for 10 days to achieve a total distraction of 10 mm, followed by a consolidation period. Animals were divided into 3 equal groups according to the injected drug (saline solution [control], zoledronic acid, alendronate). The dogs were killed 2, 6, and 10 weeks following distraction and samples were collected for radiographic examination, assessment of bone mineral density, and histopathological evaluation. Results. Radiographs and histopathological results pointed out that bone formation and maturation of experimental groups were faster than those of the control group. Conclusions. Local administration of zoledronic acid and alendronate proved to be effective in shortening the consolidation
BMC Complementary and Alternative Medicine, 2014
Background: An overdose of paracetamol is a frequent reason for liver and renal toxicity and poss... more Background: An overdose of paracetamol is a frequent reason for liver and renal toxicity and possible death and curcumin has hepatoprotective properties against liver damage. The exact mechanism of such protection is not clear. Therefore, this study was conducted to examine the molecular levels of the protective effect of curcumin on paracetamol overdose induced hepatic toxicity in rats.
International journal of immunopathology and pharmacology
Recent studies showed prominent antimicrobial activity of various plant extracts on certain patho... more Recent studies showed prominent antimicrobial activity of various plant extracts on certain pathogenic microorganisms, therefore we prepared crude aqueous extracts of black pepper, ginger and thyme and carried out an in vitro study by measuring antimicrobial activity of these extracts using the agar well diffusion method. An in vivo study was carried out on 50 adult healthy male albino rats which were divided into 5 groups, 10 rats each. Group 1: negative control group which received saline solution intragastrically daily; Group 2: Positive control group, injected with mixed bacterial suspension of S.aureus and E.coli as a model of pyelonephritis, then received saline solution intragastrically daily; Group 3: injected with the same dose of mixed bacterial suspension, then received 100 mg/kg/day black pepper extract intragastrically; Group 4: injected with mixed bacterial suspension then received 500 mg/kg/day ginger extract intragastrically. Group 5: injected with mixed bacterial su...
American Journal of Pharmacology and Toxicology, 2013
The present study was directed to evaluate the toxic effects of orally administered titanium diox... more The present study was directed to evaluate the toxic effects of orally administered titanium dioxide naonparticles (TiO2) on liver of male albino rats and to evaluate the ameliorative effects of N-acetylcysteine (NAC). Forty adult male albino rats were divided into 4 groups; control group, NAC group, TiO2 group and TiO2/ NAC group. Rats were administered either TiO2 (1200 mg kg −1 BW) or NAC (100 mg kg −1 BW) alone or together for 9 months. Blood was taken to evaluate serum changes in GPT, GOT and MDA levels. Liver tissues were examined for changes in MDA, GSH and changes in liver histopathology. Administration of TiO2 increased serum GPT, GOT and decreased MDA levels. Co-treatment of rats with NAC and TiO2 improved such significant changes induced by TiO2 alone. Moreover, significant time dependent increase in MDA and decrease in GSH levels in liver tissues were recorded. Liver histopathology showed vacuolar, hydropic degeneration and cell death of some hepatic cells. In conclusion, results confirmed the protective effect of NAC in amelioration of the biohazard effects induced by TiO2 in rats.
American Journal of Pharmacology and Toxicology, 2014
In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dio... more In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dioxide (TiO2) induced testicular degeneration in albino rats. Adult male albino rats were given saline as a control group, TiO2 (1200 mg kg −1 BW), NAC (100 mg kg −1 BW) and co-treatment of NAC and TiO2 as a protective group for 3 months. Testicular tissues were extracted for changes in testicular gene expression and histopathology. Administration of TiO2 significantly up-regulated mRNA expression of IL-6 and TNF-α and normalized by NAC administration. TiO2 administration down regulated Glutathione-S-Transferase (GST) while increased B-cell Lymphoma2 (BcL2) expressions. Co-administration of rats by NAC together with TiO2 normalized changes in GST and BcL2 expression. Expression of steroidogenesis related genes [Androgen Binding Protein (ABR), 17β-Hydroxysteroid Dehydrogenase (17β-HSD), cytochrome P450 17A (CYP17α) and aromatase] showed down regulation in TiO2 administered groups and normalized when NAC given together with TiO2. Moreover, TiO2 induced toxicity in testes that accompanied by degeneration in seminiferous tubules with congestion, oedema and cell disruption that are partially normalized by co-administration of NAC with TiO2. In conclusion, the present findings confirmed the benficial effect of NAC to prevent apoptosis in spermatogenic and sertoli cells and testicular dysfunction induced by TiO2 in male albino rats.
Background: Stevioside has been used as a medication for reducing glucose levels in diabetic pati... more Background: Stevioside has been used as a medication for reducing glucose levels in diabetic patients. The exact mode of action is
still unclear. Therefore, the current study outlined the molecular and biological roles of stevioside in treatment of diabetes.
Materials and Methods: Diabetic male Wistar rats were treated with stevioside and metformin as a medication for diabetes rats for
4 weeks. Biochemical, molecular and histopathological studies have been done to evaluate the therapeutic effect of stevioside on
minimizing levels of glucose and its related gene expression in diabetic rats.
Results: Stevioside administration normalized kidney and liver biomarkers, restored alterations in antioxidants activity and lipid
profiles. Moreover, stevioside increased insulin and leptin secretion that are decreased in diabetic rats to the normal levels. For
mRNA expression, stevioside up-regulated the expressions of PK and IRS-1 genes, which are down-regulated in diabetic rats, and
was very effective in the down-regulation of CPT-1 mRNA expression. At the cellular levels; stevioside normalized the
histopathological changes induced in pancreas.
Conclusion: Stevioside has insulin like effects and is useful for diabetic patient’s therapy.
Abstract. The present study aimed to investigate the molecular mechanism underlying the hepatopro... more Abstract. The present study aimed to investigate the molecular mechanism underlying the hepatoprotective effects of pomegranate (POM) against oxidative stress in a rat model of carbon tetrachloride (CCl4)‑induced liver damage. Injection of rats with CCl4 resulted in hepatic inflammation and lipid accumulation via the upregulation of interleukin (IL)‑6 and sterol regulatory element‑binding protein 1c (SREBP‑1c) mRNA expression. CCl4 induced downregulation of the anti‑inflammatory factors alpha 2‑macroglobulin (α‑2M) and IL‑10 in comparison with the POM treated group. In addition, CCl4 induced downregulation of superoxide dismutase (SOD), glutathione S‑transferase (GST) and catalase (CAT) expression. Conversely, prior administration of POM counteracted the deleterious alterations induced by CCl4. POM downregulated CCl4‑induced IL‑6 upregulation, normalized the increase in SREBP‑1c expression, and prevented CCl4‑induced α‑2M downregulation. POM counteracted CCL4‑induced alterations via immunosuppressive, anti‑inflammatory and regenerative effects by upregulating transforming growth factor‑β1, HSP70 and IL‑10 mRNA expression. In addition, POM increased reactive oxygen species scavenging activityby augmenting the antioxidant defense mechanism against CCl4 hepatotoxicity, as demonstrated by detecting SOD, CAT and GST expression. These results confirm that, at the molecular level, POM exerts hepatoprotective effects against CCl4‑induced oxidative stress and liver tissue damage.
The present study was conducted to test the effect of Origanum Majoranum Extract (OME) of leaves ... more The present study was conducted to test the effect of Origanum Majoranum Extract (OME)
of leaves on alterations induced in a model of type 2 diabetic rats. Adult male Wistar rats
were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg)
intraperitoneally to induce type 2 diabetic rats. Diabetic rats were given aqueous extract of
OME in a dose of 20 mg/kg orally for 3 weeks. Changes in lipid profiles, glucose, insulin,
expression of some genes related to glucose metabolism and histopathological changes in liver
and kidney were examined. Administration of OME improved and normalized dyslipidemia
recorded in type 2 diabetic rats together with reduction in glucose and insulin levels. OME
induced up-regulation in gene expression of glucose [adiponectin and glucose transporter-2
(GLUT-2)] and lipid metabolism [lipoprotein lipase (LPL)]. Moreover, OME normalized
histopathological changes occurred in liver and kidney of diabetic rats. OME decreased lipids
accumulation in liver and kidney and increased regeneration of hepatic parenchyma and
restored normal renal architecture with disappearance of fat droplets. In conclusion, OME
improved dyslipidemia associated with type 2 diabetes through regulation of genes related to
glucose and lipid metabolism.