May A . El-Sayed | Zagazig University (original) (raw)
Papers by May A . El-Sayed
Saudi Journal of Biological Sciences, 2021
The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in add... more The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in addition to the chloroform (MIC) and n-butanol (MIB) fractions were investigated for the first time using UPLC-ESI-MS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of M. imbricata (MIT) and its fractions (MIC and MIB) were determined by in vitro evaluation of their cytotoxic, antioxidant, and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract) contents followed by n-butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC 50 28.5 ± 2.0 and 35.2 ± 1.2 mg/mL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant DPPH scavenging activity with SC 50 28.4 ± 1.2 mg/mL and a remarkable lipase inhibitory activity with IC 50 54.2 ± 1.2 mg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of M. imbricata extract and its chloroform and n-butanol fractions. The results revealed that M. imbricata hydroalcoholic extract might be a prolific source of bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural alternative therapy and nutritional strategy for obesity treatment.
Egyptian Journal of Chemistry, 2020
Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical an... more Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and biological studies. n-Hexane, dichloromethane and ethyl acetate fractions of hydro alcoholic extracts of stem, leaf and fruit rind were subjected to chromatographic investigation. Thirteen compounds were isolated and identified as limettin, limonin, chrysoeriol, p-coumaric acid, scoparin, vitexin, chrysoeriol-7-O-glucoside, and hesperidin in addition to friedlin, lupeol, behenic acid, β-sitosterol and stigmasterol mixture and β-sitosterol-O-glucoside. The isolated compounds were identified by UV, EI-MS, H-NMR, CNMR, COSY, HSQC, DEPT-135, APT and HMBC. HPLC-PDA-ESI-MS/MS analysis of dichloromethane and ethyl acetate fractions afforded the identification of 90 compounds including organic acids and their glycosides, flavonoids and their HMG derivatives, coumarins and limonoids. Total phenolics and flavonoids contents were quantified by Folin-Ciocalteu and aluminum chloride potassium aceta...
Saudi Pharmaceutical Journal, 2020
Saudi Pharmaceutical Journal, 2020
Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, a... more Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, antioxi-dant, and cytotoxic properties. UPLC-ESI-MS/MS was employed as a powerful technique for the characterization of secondary metabolites from a hydroalcoholic extract, dichloromethane, and ethyl acetate fractions of P. aculeata L. aerial parts. Sixty-nine compounds (flavonoids, anthocyanins, phenolics and fatty acids) were detected and characterized; flavonoids were the abundant components in the analyzed samples. The dichloromethane fraction was rich in phenolics as vanillic acid hexoside, flavonols as 3,7-dimthylquercetin, and flavones as 3 0-hydroxymelanettin. However, the ethyl acetate fraction was rich in flavonoid-C-glycosides as luteolin-8-C-b-D-glucoside (orientin) and apigenin-8-C-glucoside (vitexin), flavonoid-O, C-diglycosides such as luteolin 7-O-[6 0 '-dihydrogalloyl]-glucosyl-8-C-pentosyl-(1 ? 2)-glucoside and 2 0 '-O-rhamnosyl isoorientin. These compounds were identified for the first time in dichlor-omethane and ethyl acetate fractions of Saudi P. aculeata L. Additionally, all the samples were assessed for antioxidant activity using DPPH radical scavenging method and for cytotoxic activity through MTT assay. Accordingly, the most active fraction was the ethyl acetate which showed the highest antioxidant activity (SC 50 = 57.4 ± 1.2 lg/mL) compared with the positive control, ascorbic acid (SC 50 = 12.4 ± 0.5 lg/mL) and moderate cytotoxicity against HepG-2 (hepato-cellular carcinoma) and MCF-7 (breast carcinoma) cell lines with IC 50 = 56.9 ± 3.1 and 95.8 ± 3.8 lg/mL, respectively compared with cisplatin (IC 50 = 3.67 ± 0.22 and 5.71 ± 0.57 lg/mL, respectively for both cell lines). The antioxidant and cytotoxic activities may be attributed to the presence of high percentage of phenolic compounds and hydroxylated flavonoids detected in ethyl acetate fraction using UPLS-ESI-MS/MS.
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50=50±5.1μ...
Journal of Chemistry, 2020
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± ...
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50=50±5.1μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50=56±1.9μg/mL) and antiobesity (IC50=95.45±1.9μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phy- tochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.
Saudi Journal of Biological Sciences, 2021
The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in add... more The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in addition to
the chloroform (MIC) and n-butanol (MIB) fractions were investigated for the first time using UPLC-ESIMS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three
organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of M. imbricata (MIT)
and its fractions (MIC and MIB) were determined by in vitro evaluation of their cytotoxic, antioxidant,
and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic
acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract)
contents followed by n-butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic
activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC50 28.5 ± 2.0
and 35.2 ± 1.2 mg/mL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant
DPPH scavenging activity with SC50 28.4 ± 1.2 mg/mL and a remarkable lipase inhibitory activity with IC50
54.2 ± 1.2 mg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of M. imbricata extract and its chloroform and n-butanol fractions. The results revealed that M. imbricata hydroalcoholic extract might be a prolific source of
bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural
alternative therapy and nutritional strategy for obesity treatment
Arabian Journal of Chemistry
The macro-and micromorphological characters of the leaf, stem, root and inflorescence of Bougainv... more The macro-and micromorphological characters of the leaf, stem, root and inflorescence of Bougainvillea 'Scarlett
O'Hara' are presented with the aim of finding their diagnostic characters by which the plant can be easily identified
in both the entire and powdered forms. The fascinating thing during the current research is the presence of bulbous
trichomres, raphide crystals of calcium oxalate and pink-purple trichomes containing betalains content protrude
from the tubular perianth, from the epidermal cells of leaves and from the bracts.
The chloroform (TNC), ethyl acetate (TNE) and n-butanol (TNB) fractions of Tephrosia nubica Bioss... more The chloroform (TNC), ethyl acetate (TNE) and n-butanol (TNB) fractions of Tephrosia nubica Bioss. growing in Saudi Arabia were investigated for the first time using UPLC-ESI-MS/MS in two ionization modes. The analysis revealed the tentative identification of 107 compounds. Moreover, the therapeutic potential of T. nubica fractions was determined by in vitro evaluation of their cytotox-ic, antioxidant, and anti-obesity activities using MTT assay, DPPH radical scavenging activity and pancreatic lipase inhibitory assay, respectively. The results showed that TNE, TNB, TNC fractions revealed weak antioxidant activity with SC 50 139.9 ± 0.8, 144.9 ± 1.5, 148.9 ± 1.3 mg/mL, respectively compared to ascorbic acid 14.2 ± 0.5 mg/ml. Moreover TNE, TNC fractions showed more significant cytotoxic activity against HepG-2 with IC 50 82.1 ± 3.1, 101 ± 2.8 mg/mL and MCF-7 with IC 50 114 ± 3.2, 124 ± 3.9 mg/mL respectively. The TNB fraction showed weak cytotoxic activity against both cell lines compared to the other fractions. Ultimately, TNE fraction showed a remarkable anti-obesity activity with IC 50 62.4 ± 1.5 mg/mL compared to chloroform fraction with IC 50 535.6 ± 2.1 mg/mL and n-butanol fraction which did not show any activity. In conclusion, these findings represent the first insights into the phytochemical constituents and pharmacological properties of T. nubica. The ethyl acetate fraction of T. nubica might be a promising source of functional constituents with antioxidant, cytotoxic and anti-obesity potentials. It might be a natural alternative therapy and nutritional strategy, for obesity treatment without dangerous side effects. Isolation of the bioactive compounds from the ethyl acetate fraction of T. nubica and evaluating their biological activities are recommended.
Food and function, 2020
The stem (S), leaf (L) and fruit peel (P) of Murcott mandarins were separately extracted using 80... more The stem (S), leaf (L) and fruit peel (P) of Murcott mandarins were separately extracted using 80% ethanol and then fractionated into dichloromethane (DCM) and ethyl acetate (ET). Their metabolic profiles were studied via HPLC-PDA-ESI-MS/MS and afforded a tentative characterization of 98 compounds, including free organic acids, phenolic acid derivatives, flavonoid aglycones, flavonoid glycosides, flavonoids containing 3-hydroxyl-3-methylglutaroyl (HMG) units, coumarin derivatives and limonoids. Column chromatography resulted in isolation of six metabolites for the first time that were identified as nobiletin (C1), isosinensetin (C2), limonin (C3), 4'-demethylnobiletin (C4), stigmasterol-O-glucoside (C5) and hesperidin (C6). In vitro studies of the anti-inflammatory activity of DCM-L against cyclooxygenases (COXs) and 5-lipoxygenase (5-LOX) enzymes revealed that DCM-L showed higher activity than the other tested fractions. The in vivo gastroprotective effects of that fraction were evaluated using alcohol-induced gastric ulcers in rats. The obtained findings validated the gastroprotective and anti-ulcerogenic activities of DCM-L through its anxiolytic, anti-inflammatory, antioxidant and anti-apoptotic effects. Therefore, we recommend the use of Murcott mandarin leaves as a part of a protection strategy for gastric ulcer.
The ethyl acetate fraction of Lavandula coronopifolia is an affordable source for compounds which... more The ethyl acetate fraction of Lavandula coronopifolia is an affordable source for compounds which have antioxidant activity and also cytotoxic activity againt hepatic and breast cancers. Please cite this paper as: Abdelaziz S, Hassan WHB, Elhassanny AEM, Al-Yousef HM, Elsayed MA, Adel R. Ultra performance liquid chromatography-tandem mass spectrometeric analysis of ethyl acetate fraction from saudi Lavandula coronopifolia Poir and evaluation of its cytotoxic and antioxidant activities. Introduction: The ethyl acetate fraction of the Saudi Lavandula coronopifolia Poir has been previously reported to have hepatoprotective activity against ethanol-induced oxidative stress. The aim of the current study was to investigate the chemical composition, cytotoxic effect, and antioxidant activities of ethyl acetate fraction of the aerial parts of Saudi L. coronopifolia Poir. Methods: Air dried aerial parts of L. coronopifolia were extracted using 90% ethyl alcohol. The dried extract was suspended in water, defatted with light petroleum and then fractionated with ethyl acetate. The ethyl acetate fraction was subjected to ultra performance liquid chromatography-tandem mass spectrometeric (UPLC-ESI/MS/MS) analysis in a negative ionization mode. The antioxidant activity of the fraction was determined using free radical 2,2-diphyenyl-picrylhydrazyl (DPPH) scavenging assay and its cytotoxic effect against HepG-2 (human hepatocarcinoma) and MCF-7 (human breast carcinoma) cells were determined using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) cell viability assay. Results: The major components of the ethyl acetate fraction included carvacrol-O-diglucoside, (34.98%) and trihydroxy ursolic acid (12.07%). Moreover, the DPPH radical scavenging activity of ethyl acetate fraction was measured. The ethyl acetate fraction revealed an antioxidant potential with EC 50 17.8 ± 1.3 µg/mL. Additionally, the ethyl acetate fraction showed cytotoxic activity against HepG-2 and MCF-7 cells with IC 50 =29.3 ± 0.9 µg/mL and 14.6 ± 0.3 µg/mL, respectively. Conclusion: The ethyl acetate fraction of the Saudi L. coronopifolia has antioxidant activity and also cytotoxic activity against breast and liver cancer cells.
Journal of Pharmaceutical Sciences and Research, 2017
Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and bi... more Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and biological studies. n-Hexane, dichloromethane and ethyl acetate fractions of hydro alcoholic extracts of stem, leaf and fruit rind were subjected to chromatographic investigation. Thirteen compounds were isolated and identified as limettin, limonin, chrysoeriol, p-coumaric acid, scoparin, vitexin, chrysoeriol-7-O-glucoside, and hesperidin in addition to friedlin, lupeol, behenic acid, β-sitosterol and stigmasterol mixture and β-sitosterol-O-glucoside. The isolated compounds were identified by UV, EI-MS, 1 H-NMR, 13 C-NMR, COSY, HSQC, DEPT-135, APT and HMBC. HPLC-PDA-ESI-MS/MS analysis of dichloromethane and ethyl acetate fractions afforded the identification of 90 compounds including organic acids and their glycosides, flavonoids and their HMG derivatives, coumarins and limonoids. Total phenolics and flavonoids contents were quantified by Folin-Ciocalteu and aluminum chloride-potassium acetate colorimetric methods, respectively. Ethyl acetate fraction and total alcoholic extract of stem showed the highest phenolics (113.01 ± 0.23 mg GAE/g extract) and flavonoids (557.10 ± 0.82 mg quercetin and 625.22 ± 0.91 mg rutin equivalent/g extract) concentrations, respectively. Antioxidant activity was evaluated using DPPH radical scavenging capacity where leaf ethyl acetate fraction showed the highest activity (SC 50 =19.01µg/mL) compared with ascorbic acid (SC 50 =13.4 µg/mL). Antimicrobial activity was assayed by agar well diffusion and MIC for different fractions where rind and leaf ethyl acetate fractions exceeded activity of gentamycin against Klebsiella pneumoniae. Additionally, Juice and rind ethyl acetate showed the best activity against Bacillus subtilis (MIC=0.49 µg/mL) and Saccharomyces cerevisiae (MIC=0.98 µg/mL). Moreover, Enterococcus faecalis was highly sensitive to juice ethyl acetate (MIC=0.49 µg/mL).
Hydrodistilled essential oils isolated from the leaf, ripe and unripe rinds as well as flower hex... more Hydrodistilled essential oils isolated from the leaf, ripe and unripe rinds as well as flower hexane extract of Murcott mandarin were analysed by GLC-MS to identify their constituents. The identified compounds were 48, 41, 40 and 46 from the mentioned organs, respectively. Monoterpenes represented the highest percentage for the identified components of ripe rind (94.76%), unripe rind (97.05%) and flower hexane fraction (50.97%) while oxygenated monoterpenes (45.94%) were the highest for leaf oil. Limonene was the major components in all samples followed by terpinene-4-ol and linalool in leaf oil, geranial, γ-terpinen and neral in flower hexane extract. Myrcene represented 2.43% and 2.69% for the ripe and unripe rind, respectively. Moreover, the major constituents were quantified by GLC-FID using a calibration curve of limonene. All tested samples showed high concentration of limonene which reached its highest concentration in flower hexane fraction (527.54 µg/ml). The tested samples were evaluated for their antimicrobial activities by using agar well diffusion assay and determination of minimum inhibitory concentration (MIC) using gentamicin, ampicillin and amphotricin B as positive controls. Flower hexane extract showed the best activity against Enterococcus faecalis while leaf oil exhibited its highest activity against Enterobacter cloacae and Bacillus subtilis. Additionally, Klebsiella pneumonia, Staphylococcus aureus, and Aspragillus fumigatus were the most sensitive to ripe rind oil while Saccharomyces cerevisiae was greatly inhibited by unripe rind (MIC = 1.95 µg /ml for all). Escherichia coli showed equal responses towards ripe and unripe rind oils (MIC=3.9 µg/ml). Also, ripe rind and leaf oils exhibited equal inhibitory effect against B. subtilis. MTT assay was used to evaluate cytotoxic activity compared to doxorubicin. Leaf oil showed the most potent effect on human lung carcinoma (A-549) cell line with IC 50 = 2.5 μg, while unripe rind oil exhibited the highest activity on human colon carcinoma (HCT-116) and hepatocellular carcinoma (Hep-G2) cell lines with IC 50 = 2.98 and 3.62 μg, respectively. The results recommend the use of Murcott mandarin oils as food preservatives and need further studies for the possible use as anticancer agents.
Saudi Journal of Biological Sciences, 2021
The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in add... more The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in addition to the chloroform (MIC) and n-butanol (MIB) fractions were investigated for the first time using UPLC-ESI-MS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of M. imbricata (MIT) and its fractions (MIC and MIB) were determined by in vitro evaluation of their cytotoxic, antioxidant, and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract) contents followed by n-butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC 50 28.5 ± 2.0 and 35.2 ± 1.2 mg/mL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant DPPH scavenging activity with SC 50 28.4 ± 1.2 mg/mL and a remarkable lipase inhibitory activity with IC 50 54.2 ± 1.2 mg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of M. imbricata extract and its chloroform and n-butanol fractions. The results revealed that M. imbricata hydroalcoholic extract might be a prolific source of bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural alternative therapy and nutritional strategy for obesity treatment.
Egyptian Journal of Chemistry, 2020
Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical an... more Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and biological studies. n-Hexane, dichloromethane and ethyl acetate fractions of hydro alcoholic extracts of stem, leaf and fruit rind were subjected to chromatographic investigation. Thirteen compounds were isolated and identified as limettin, limonin, chrysoeriol, p-coumaric acid, scoparin, vitexin, chrysoeriol-7-O-glucoside, and hesperidin in addition to friedlin, lupeol, behenic acid, β-sitosterol and stigmasterol mixture and β-sitosterol-O-glucoside. The isolated compounds were identified by UV, EI-MS, H-NMR, CNMR, COSY, HSQC, DEPT-135, APT and HMBC. HPLC-PDA-ESI-MS/MS analysis of dichloromethane and ethyl acetate fractions afforded the identification of 90 compounds including organic acids and their glycosides, flavonoids and their HMG derivatives, coumarins and limonoids. Total phenolics and flavonoids contents were quantified by Folin-Ciocalteu and aluminum chloride potassium aceta...
Saudi Pharmaceutical Journal, 2020
Saudi Pharmaceutical Journal, 2020
Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, a... more Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, antioxi-dant, and cytotoxic properties. UPLC-ESI-MS/MS was employed as a powerful technique for the characterization of secondary metabolites from a hydroalcoholic extract, dichloromethane, and ethyl acetate fractions of P. aculeata L. aerial parts. Sixty-nine compounds (flavonoids, anthocyanins, phenolics and fatty acids) were detected and characterized; flavonoids were the abundant components in the analyzed samples. The dichloromethane fraction was rich in phenolics as vanillic acid hexoside, flavonols as 3,7-dimthylquercetin, and flavones as 3 0-hydroxymelanettin. However, the ethyl acetate fraction was rich in flavonoid-C-glycosides as luteolin-8-C-b-D-glucoside (orientin) and apigenin-8-C-glucoside (vitexin), flavonoid-O, C-diglycosides such as luteolin 7-O-[6 0 '-dihydrogalloyl]-glucosyl-8-C-pentosyl-(1 ? 2)-glucoside and 2 0 '-O-rhamnosyl isoorientin. These compounds were identified for the first time in dichlor-omethane and ethyl acetate fractions of Saudi P. aculeata L. Additionally, all the samples were assessed for antioxidant activity using DPPH radical scavenging method and for cytotoxic activity through MTT assay. Accordingly, the most active fraction was the ethyl acetate which showed the highest antioxidant activity (SC 50 = 57.4 ± 1.2 lg/mL) compared with the positive control, ascorbic acid (SC 50 = 12.4 ± 0.5 lg/mL) and moderate cytotoxicity against HepG-2 (hepato-cellular carcinoma) and MCF-7 (breast carcinoma) cell lines with IC 50 = 56.9 ± 3.1 and 95.8 ± 3.8 lg/mL, respectively compared with cisplatin (IC 50 = 3.67 ± 0.22 and 5.71 ± 0.57 lg/mL, respectively for both cell lines). The antioxidant and cytotoxic activities may be attributed to the presence of high percentage of phenolic compounds and hydroxylated flavonoids detected in ethyl acetate fraction using UPLS-ESI-MS/MS.
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50=50±5.1μ...
Journal of Chemistry, 2020
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± ...
The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus defle... more The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50=50±5.1μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50=56±1.9μg/mL) and antiobesity (IC50=95.45±1.9μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phy- tochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.
Saudi Journal of Biological Sciences, 2021
The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in add... more The hydroalcoholic extract (MIT) of Micromeria imbricata (Forssk.) growing in Saudi Arabia in addition to
the chloroform (MIC) and n-butanol (MIB) fractions were investigated for the first time using UPLC-ESIMS/MS. The analysis revealed the tentative identification of fifty-eight compounds including three
organic acids, twenty-five phenolic compounds, three coumarins, two anthocyanins, twenty-one flavonoids, three terpenes, and one miscellaneous. Moreover, the therapeutic potential of M. imbricata (MIT)
and its fractions (MIC and MIB) were determined by in vitro evaluation of their cytotoxic, antioxidant,
and anti-obesity characteristics. The MIT extract showed the highest phenolic (125.23 ± 0.87 mg gallic
acid equivalent/100 gm extract) and flavonoid (112.24 ± 2.45 mg quercetin equivalent/100 gm extract)
contents followed by n-butanol and chloroform fractions. The MIT extract revealed a potent cytotoxic
activity against HepG-2 (Hepatocellular carcinoma) and MCF-7 (Breast carcinoma) with IC50 28.5 ± 2.0
and 35.2 ± 1.2 mg/mL, respectively. Additionally, the tested hydroalcoholic extract exhibited a significant
DPPH scavenging activity with SC50 28.4 ± 1.2 mg/mL and a remarkable lipase inhibitory activity with IC50
54.2 ± 1.2 mg/mL. In conclusion, the current study presents the first insights into the phytochemical constituents and pharmacological properties of M. imbricata extract and its chloroform and n-butanol fractions. The results revealed that M. imbricata hydroalcoholic extract might be a prolific source of
bioactive constituents with potent antioxidant, cytotoxic and anti-obesity potential. It might be a natural
alternative therapy and nutritional strategy for obesity treatment
Arabian Journal of Chemistry
The macro-and micromorphological characters of the leaf, stem, root and inflorescence of Bougainv... more The macro-and micromorphological characters of the leaf, stem, root and inflorescence of Bougainvillea 'Scarlett
O'Hara' are presented with the aim of finding their diagnostic characters by which the plant can be easily identified
in both the entire and powdered forms. The fascinating thing during the current research is the presence of bulbous
trichomres, raphide crystals of calcium oxalate and pink-purple trichomes containing betalains content protrude
from the tubular perianth, from the epidermal cells of leaves and from the bracts.
The chloroform (TNC), ethyl acetate (TNE) and n-butanol (TNB) fractions of Tephrosia nubica Bioss... more The chloroform (TNC), ethyl acetate (TNE) and n-butanol (TNB) fractions of Tephrosia nubica Bioss. growing in Saudi Arabia were investigated for the first time using UPLC-ESI-MS/MS in two ionization modes. The analysis revealed the tentative identification of 107 compounds. Moreover, the therapeutic potential of T. nubica fractions was determined by in vitro evaluation of their cytotox-ic, antioxidant, and anti-obesity activities using MTT assay, DPPH radical scavenging activity and pancreatic lipase inhibitory assay, respectively. The results showed that TNE, TNB, TNC fractions revealed weak antioxidant activity with SC 50 139.9 ± 0.8, 144.9 ± 1.5, 148.9 ± 1.3 mg/mL, respectively compared to ascorbic acid 14.2 ± 0.5 mg/ml. Moreover TNE, TNC fractions showed more significant cytotoxic activity against HepG-2 with IC 50 82.1 ± 3.1, 101 ± 2.8 mg/mL and MCF-7 with IC 50 114 ± 3.2, 124 ± 3.9 mg/mL respectively. The TNB fraction showed weak cytotoxic activity against both cell lines compared to the other fractions. Ultimately, TNE fraction showed a remarkable anti-obesity activity with IC 50 62.4 ± 1.5 mg/mL compared to chloroform fraction with IC 50 535.6 ± 2.1 mg/mL and n-butanol fraction which did not show any activity. In conclusion, these findings represent the first insights into the phytochemical constituents and pharmacological properties of T. nubica. The ethyl acetate fraction of T. nubica might be a promising source of functional constituents with antioxidant, cytotoxic and anti-obesity potentials. It might be a natural alternative therapy and nutritional strategy, for obesity treatment without dangerous side effects. Isolation of the bioactive compounds from the ethyl acetate fraction of T. nubica and evaluating their biological activities are recommended.
Food and function, 2020
The stem (S), leaf (L) and fruit peel (P) of Murcott mandarins were separately extracted using 80... more The stem (S), leaf (L) and fruit peel (P) of Murcott mandarins were separately extracted using 80% ethanol and then fractionated into dichloromethane (DCM) and ethyl acetate (ET). Their metabolic profiles were studied via HPLC-PDA-ESI-MS/MS and afforded a tentative characterization of 98 compounds, including free organic acids, phenolic acid derivatives, flavonoid aglycones, flavonoid glycosides, flavonoids containing 3-hydroxyl-3-methylglutaroyl (HMG) units, coumarin derivatives and limonoids. Column chromatography resulted in isolation of six metabolites for the first time that were identified as nobiletin (C1), isosinensetin (C2), limonin (C3), 4'-demethylnobiletin (C4), stigmasterol-O-glucoside (C5) and hesperidin (C6). In vitro studies of the anti-inflammatory activity of DCM-L against cyclooxygenases (COXs) and 5-lipoxygenase (5-LOX) enzymes revealed that DCM-L showed higher activity than the other tested fractions. The in vivo gastroprotective effects of that fraction were evaluated using alcohol-induced gastric ulcers in rats. The obtained findings validated the gastroprotective and anti-ulcerogenic activities of DCM-L through its anxiolytic, anti-inflammatory, antioxidant and anti-apoptotic effects. Therefore, we recommend the use of Murcott mandarin leaves as a part of a protection strategy for gastric ulcer.
The ethyl acetate fraction of Lavandula coronopifolia is an affordable source for compounds which... more The ethyl acetate fraction of Lavandula coronopifolia is an affordable source for compounds which have antioxidant activity and also cytotoxic activity againt hepatic and breast cancers. Please cite this paper as: Abdelaziz S, Hassan WHB, Elhassanny AEM, Al-Yousef HM, Elsayed MA, Adel R. Ultra performance liquid chromatography-tandem mass spectrometeric analysis of ethyl acetate fraction from saudi Lavandula coronopifolia Poir and evaluation of its cytotoxic and antioxidant activities. Introduction: The ethyl acetate fraction of the Saudi Lavandula coronopifolia Poir has been previously reported to have hepatoprotective activity against ethanol-induced oxidative stress. The aim of the current study was to investigate the chemical composition, cytotoxic effect, and antioxidant activities of ethyl acetate fraction of the aerial parts of Saudi L. coronopifolia Poir. Methods: Air dried aerial parts of L. coronopifolia were extracted using 90% ethyl alcohol. The dried extract was suspended in water, defatted with light petroleum and then fractionated with ethyl acetate. The ethyl acetate fraction was subjected to ultra performance liquid chromatography-tandem mass spectrometeric (UPLC-ESI/MS/MS) analysis in a negative ionization mode. The antioxidant activity of the fraction was determined using free radical 2,2-diphyenyl-picrylhydrazyl (DPPH) scavenging assay and its cytotoxic effect against HepG-2 (human hepatocarcinoma) and MCF-7 (human breast carcinoma) cells were determined using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) cell viability assay. Results: The major components of the ethyl acetate fraction included carvacrol-O-diglucoside, (34.98%) and trihydroxy ursolic acid (12.07%). Moreover, the DPPH radical scavenging activity of ethyl acetate fraction was measured. The ethyl acetate fraction revealed an antioxidant potential with EC 50 17.8 ± 1.3 µg/mL. Additionally, the ethyl acetate fraction showed cytotoxic activity against HepG-2 and MCF-7 cells with IC 50 =29.3 ± 0.9 µg/mL and 14.6 ± 0.3 µg/mL, respectively. Conclusion: The ethyl acetate fraction of the Saudi L. coronopifolia has antioxidant activity and also cytotoxic activity against breast and liver cancer cells.
Journal of Pharmaceutical Sciences and Research, 2017
Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and bi... more Variegated pink lemon is a valuable citrus tree, which didn't receive enough phytochemical and biological studies. n-Hexane, dichloromethane and ethyl acetate fractions of hydro alcoholic extracts of stem, leaf and fruit rind were subjected to chromatographic investigation. Thirteen compounds were isolated and identified as limettin, limonin, chrysoeriol, p-coumaric acid, scoparin, vitexin, chrysoeriol-7-O-glucoside, and hesperidin in addition to friedlin, lupeol, behenic acid, β-sitosterol and stigmasterol mixture and β-sitosterol-O-glucoside. The isolated compounds were identified by UV, EI-MS, 1 H-NMR, 13 C-NMR, COSY, HSQC, DEPT-135, APT and HMBC. HPLC-PDA-ESI-MS/MS analysis of dichloromethane and ethyl acetate fractions afforded the identification of 90 compounds including organic acids and their glycosides, flavonoids and their HMG derivatives, coumarins and limonoids. Total phenolics and flavonoids contents were quantified by Folin-Ciocalteu and aluminum chloride-potassium acetate colorimetric methods, respectively. Ethyl acetate fraction and total alcoholic extract of stem showed the highest phenolics (113.01 ± 0.23 mg GAE/g extract) and flavonoids (557.10 ± 0.82 mg quercetin and 625.22 ± 0.91 mg rutin equivalent/g extract) concentrations, respectively. Antioxidant activity was evaluated using DPPH radical scavenging capacity where leaf ethyl acetate fraction showed the highest activity (SC 50 =19.01µg/mL) compared with ascorbic acid (SC 50 =13.4 µg/mL). Antimicrobial activity was assayed by agar well diffusion and MIC for different fractions where rind and leaf ethyl acetate fractions exceeded activity of gentamycin against Klebsiella pneumoniae. Additionally, Juice and rind ethyl acetate showed the best activity against Bacillus subtilis (MIC=0.49 µg/mL) and Saccharomyces cerevisiae (MIC=0.98 µg/mL). Moreover, Enterococcus faecalis was highly sensitive to juice ethyl acetate (MIC=0.49 µg/mL).
Hydrodistilled essential oils isolated from the leaf, ripe and unripe rinds as well as flower hex... more Hydrodistilled essential oils isolated from the leaf, ripe and unripe rinds as well as flower hexane extract of Murcott mandarin were analysed by GLC-MS to identify their constituents. The identified compounds were 48, 41, 40 and 46 from the mentioned organs, respectively. Monoterpenes represented the highest percentage for the identified components of ripe rind (94.76%), unripe rind (97.05%) and flower hexane fraction (50.97%) while oxygenated monoterpenes (45.94%) were the highest for leaf oil. Limonene was the major components in all samples followed by terpinene-4-ol and linalool in leaf oil, geranial, γ-terpinen and neral in flower hexane extract. Myrcene represented 2.43% and 2.69% for the ripe and unripe rind, respectively. Moreover, the major constituents were quantified by GLC-FID using a calibration curve of limonene. All tested samples showed high concentration of limonene which reached its highest concentration in flower hexane fraction (527.54 µg/ml). The tested samples were evaluated for their antimicrobial activities by using agar well diffusion assay and determination of minimum inhibitory concentration (MIC) using gentamicin, ampicillin and amphotricin B as positive controls. Flower hexane extract showed the best activity against Enterococcus faecalis while leaf oil exhibited its highest activity against Enterobacter cloacae and Bacillus subtilis. Additionally, Klebsiella pneumonia, Staphylococcus aureus, and Aspragillus fumigatus were the most sensitive to ripe rind oil while Saccharomyces cerevisiae was greatly inhibited by unripe rind (MIC = 1.95 µg /ml for all). Escherichia coli showed equal responses towards ripe and unripe rind oils (MIC=3.9 µg/ml). Also, ripe rind and leaf oils exhibited equal inhibitory effect against B. subtilis. MTT assay was used to evaluate cytotoxic activity compared to doxorubicin. Leaf oil showed the most potent effect on human lung carcinoma (A-549) cell line with IC 50 = 2.5 μg, while unripe rind oil exhibited the highest activity on human colon carcinoma (HCT-116) and hepatocellular carcinoma (Hep-G2) cell lines with IC 50 = 2.98 and 3.62 μg, respectively. The results recommend the use of Murcott mandarin oils as food preservatives and need further studies for the possible use as anticancer agents.