ZFIN The Zebrafish Information Network (original) (raw)

Figure 5 of Sobah et al., 2023

Effect of Stat3 mutations on embryonic cartilage formation. Representative images of STAT3 wildtype (WT: A,D,G,J,M,P), knockout (KO: B,E,H,K,N,Q), and transactivation domain truncation (ΔTAD: C,F,I,L,O,R) mutant embryos subjected to WISH at 50 hpf with dlx2a (lateral: A–C; ventral: D–F) and at 72 hpf with col2a1a (lateral: G–I; ventral: J–L), or at 5 dpf to Alcian blue staining (lateral: M–O; ventral: P–R) with scale bars representing 200 μm.

Fig 2 of Rayamajhi et al., 2024

Foxj1 regulates olfactory cilia biogenesis in zebrafish and OE establishment in mice.(A) Immunostaining with anti-Gαolf antibody marking olfactory cilia (magenta) in foxj1a,b mutant zebrafish larvae at 4 dpf. Nuclei marked with DAPI (blue). foxj1a mutants showed no observable effect on the formation of olfactory cilia (n = 3). foxj1b mutants showed reduced olfactory cilia (n = 3). foxj1a/b double mutants showed severe loss of olfactory cilia (n = 3). (B, B’) Innervation pattern of ciliated OSNs labeled with Gαolf (magenta) in control (n = 3) and foxj1a/b double mutant (n = 5) showed no significant effect in the formation of olfactory glomeruli at 4 dpf. Nuclei marked with DAPI (blue). (C) foxj1a/b double mutant showing severe reduction of motile cilia number in comparison to the foxj1a mutants, foxj1b mutants and control, as shown by immunostaining with anti-acetylated-tubulin for marking cilia (yellow) and beta-catenin for marking cell borders (magenta) at 4 dpf. (nControl = 13, nfoxj1a−/− = 20, nfoxj1b−/− = 22, nfoxj1a−/−; foxj1b−/− = 9). Nuclei are marked with DAPI (blue). (D) Significant decrease in the size of nose and nasal cavity, and number of MCCs in foxj1b and foxj1a/b double mutant embryos. (For nose size, nControl = 13, nfoxj1a−/− = 20, nfoxj1b−/− = 22, nfoxj1a−/−; foxj1b−/− = 9. For number of MCCs, nControl = 9, nfoxj1a−/− = 5, nfoxj1b−/− = 5, nfoxj1a−/−; foxj1b−/− = 6). (E) The OE of P21 mouse was stained for OMP (green), a mature OSN marker. Apical layer, composed of mucus and cilia, is strongly labeled for acetylated α-tubulin (magenta) in control (top, arrow). Mature OSNs (OMP, green) at the same animal age of 21 day were fewer in number and disorganized within the OE of the Foxj1−/− mouse (bottom), (10,630 ± 923 cells per mm2, n = 8, WT; 3,106 ± 714, n = 7, KO, 3 mice, p = 0.0006). Compared to the WT, thickness of the OE was significantly reduced in the Foxj1−/− mutant (79.68 ± 4.31 μm, n = 34, 3 mice, WT; 45.48 ± 2.15 μm, n = 30, 3 mice, p < 0.0001, KO). (F) Immature OSNs (GAP43, magenta) were located below the layer of mature OSNs (OMP, green) as shown in a control mouse (top). Immature OSNs (GAP43, magenta) lost their orientation relative to mature OSNs and were fewer in numbers within the OE of the Foxj1−/− mutant (bottom) as compared to control (6,339 ± 1,015 cells per mm2, n = 8, WT; 2,008 ± 481, n = 7, KO, 3 mice, p = 0.0012). The nasal cavity (E-G, asterisks) was unobstructed in the WT, whereas in the Foxj1−/− animals, it was completely filled with DAPI and Ki67-positive cells. (G) Proliferating cells expressing Ki67 (magenta) were mostly comprised of basal progenitor cells lining lamina propria. Fewer proliferating cells (Ki67, magenta) were present in the OE of the Foxj1−/− mouse (bottom) as compared to the control (top) (2,362 ± 321 cells per mm2, n = 9, WT; 502 ± 72, n = 9, KO, 3 mice, p < 0.0001). (H) Images showing the OB having oval-shaped glomeruli (top) filled with the axonal projections of OSNs (OMP, white) in control (top). Reduced intensity of OMP immunostaining in the Foxj1−/− mouse (bottom) as compared to the control (top) (352.5 ± 26.7 a.u., n = 21, WT; 266.6 ± 17.5 a.u., n = 44, KO; 3 mice, p = 0.0133). In the Foxj1−/− mouse, glomeruli overall were less developed and had smaller perimeter than in the WT (2,558 ± 368 μm, n = 21, 2 mice, WT; 1,331 ± 306 μm, n = 44, 2 mice, KO; 3 mice, p < 0.0001). Scale bars: 10 μm (A-D), 50 μm (E-H). Raw data files are available in Mendeley Data (https://data.mendeley.com/datasets/2pn963jn6y). dpf, days post fertilization; KO, knockout; MCC, motile multiciliated cell; OB, olfactory bulb; OE, olfactory epithelium; OMP, olfactory marker protein; OSN, olfactory sensory neuron; WT, wild type.

Fig 4 of Rayamajhi et al., 2024

Olfactory response to bile acid is reduced in foxj1 mutant zebrafish larvae.(A) Schematic of the olfactory experiment, showing a 4-dpf zebrafish larva embedded in agarose. Its nose was exposed, and odor stimuli were delivered by a fine tube. (B) Neural activity was measured using the Ca2+-reporter GCaMP6s (Tg(elavl3:Gcamp6s)) in a region of interest spanning the entire OE. (C, C’) Representative example showing neural activity in the OE and OB to various odor types. Note that responses in the OB were spatially organized, with nonoverlapping domains. (D-D””) Averaged traces of neural activity on the OE for foxj1b control (n = 12) and mutant (n = 8) fish for each odor type showed no difference in odor responses. Maximum amplitude is shown in the insets. (E-E””) Averaged traces of neural activity in the OE for control (n = 7), foxj1a mutant (n = 10), and double foxj1a/b mutant (n = 14) showed a significant reduction in bile acid response. Shaded error bars are standard error of the mean. Maximum amplitude and standard deviation are shown in the insets. Significance identified by two-sample t test, *: p < 0.05, **: p < 0.01. Raw data files and codes for analysis are available in Mendeley Data (https://data.mendeley.com/datasets/2pn963jn6y). a, anterior; AA, amino acid; BA, bile acid; dpf, days post fertilization; FO, food odor; NU, nucleic acid; OB, olfactory bulb; OE, olfactory epithelium; p, posterior.

Figure 7 of Taler et al., 2023

mTOR signaling contributes to cell mass growth. (A,B) Confocal single-plane sections from eyes of 3 dpf plod3vu222 larvae labeled for pS6K (green) and with 5E11 antibody that marks nuclei of ALE cells as well as a subset of amacrine cells (purple). pS6K staining in the mutant is increased in the developing mass and reduced in the ciliary marginal zones (marked by asterisks in (A)). White rectangles mark the ALE region. Scale bar is 50 µm. (C,D) Histological sections from eyes of 4 dpf plod3vu222 larvae treated from 26 hpf until ~96 hpf with vehicle (C) or rapamycin (D). White lines mark the periphery of cellular masses. Scale bar is 20 µm. L, lens.

Fig. 2 of Dennhag et al., 2024

fhl2b is upregulated in the EOM in response to desmin-related muscular dystrophy.a Expression of myofiber-related DEGs for the following comparisons: 5 months desma−/−:desmb−/−vs WT EOMs (I), 5 months desma−/−:desmb−/−vs WT trunk (II), 20 months desma−/−:desmb−/−vs WT EOMs (III), 20 months desma−/−:desmb−/−vs WT trunk (IV), 5 months WT EOMs vs WT trunk (V), 5 months desma−/−:desmb−/− EOMs vs desma−/−:desmb−/− trunk (VI), 20 months WT EOMs vs WT trunk (VII) and 20 months desma−/−:desmb−/− EOMs vs desma−/−:desmb−/− trunk (VIII). bfhl2b expression in the abovementioned comparisons I-VIII. FHL2 antibody labeling of WT and desma−/−;desmb−/− cross-sectioned EOMs at c 5 and d 20 months. e FHL2 positive myofibers quantification of desma−/−;desmb−/− versus WT control EOMs in 5 (p = 0.017) and 20-monthold zebrafish (p = 3.7e−7), respectively and 5-months versus 20-months-old WT (p = 0.014) and desma−/−:desmb−/− (p = 0.005), respectively. Data is presented as median (line) and quartiles (dashed lines). f WT, plecb−/− and obscnb−/− 12-month-old EOM cross sections immunolabeled with FHL2 antibodies. g Human EOM cross section immunolabeled with DAPI/FHL2/laminin antibodies, dashed square indicates area enlarged below. h Western blot on human EOMs showing FHL2. i Mouse EOM cross section immunolabeled using DAPI/FHL2/laminin antibodies, dashed boxes indicate area enlarged below. j Western blot of mouse EOMs showing FHL2. Statistical analysis in b: Two-sided Wald test with B/H-correction, e: Two-sided t-tests with Welch correction. Scale bars in c, d, f, g, i: 50 µm, g, i bottom panel: 25 µm. White dashed lines outline the entire cross-section of the EOMs. Schematic images were adapted from https://www.biorender.com.