DOK (original) (raw)

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

DOK (human, Caucasian, tongue, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: tongue
TUMOR: carcinoma

ECACC catalog code: 94122104
Morphology: Epithelial
Description: Human Caucasian dysplastic oral keratinocyte
Viruses: See Properties
Depositor: Dr S E Chang, Department of Virology, Royal Postgraduate Medical School, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: p53 Applications: Study of oral keratinocytes, multi-stage oral carcinogenesis and carcinogens

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 5�g/ml Hydrocortisone + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 24; Karyotype: Aneuploid
  The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Br J Cancer 1994;70:591 - PMID: 7917902
• Int J Cancer 1992;52:896 - PMID: 1459732