EL4 (original) (raw)

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EL4 (mouse, blood, lymphoma)

SPECIES: mouse
TISSUE/ORGAN: blood
TUMOR: lymphoma

ECACC catalog code: 85023105
Morphology: Lymphoblast
Description: Mouse ascites lymphoma lymphoblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2 Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
  Karyotype: 2n = 39
  Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol (10e4M) and dexamethasone and is sensitive to PHA.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB-39

Bibliographic references:

• Br J Cancer 1950;4:372
• Cancer Res 1965;25:813 - PMID: 4284252
• J Natl Cancer Inst 1972;48:265 - PMID: 4119883

See homonymous cell lines in other collections and catalogues.