FBHE (original) (raw)

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FBHE (bovine, heart, embryonic)

SPECIES: bovine
TISSUE/ORGAN: heart, embryonic

ECACC catalog code: 87043002
Morphology: Endothelial
Description: Bovine embryo heart
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 100-200ng/ml/day Fibroblast Growth Factor (FGF) + 10% Fetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hrs and wash with PBS. Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2 37C.
  Passages: 12; The cells have an absolute dependency on fibroblast growth factor for survival. The cells are BVDV negative. Fresh Fibroblast growth factor (FGF) should be added daily.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1395, IZSBS: BS CL113

Bibliographic references:

• Proc Natl Acad Sci U S A 1976;73:4120 - PMID: 1069301

See homonymous cell lines in other collections and catalogues.