8505C (original) (raw)

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8505C (human, thyroid, carcinoma, undifferentiated)

SPECIES: human
TISSUE/ORGAN: thyroid
TUMOR: carcinoma, undifferentiated

ECACC catalog code: 94090184
Morphology: Epithelial
Description: Human thyroid carcinoma, undifferentiated.
Viruses: See Properties
Depositor: Dr M Akiyama, Radiobiology Dept, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima 732, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: As p53 mutations associate with undifferentiated carcinoma but not well differentiated carcinoma during multistep carcinogenesis of the thyroid, these cell lines should prove useful for research into the role of gene mutation in malignant transformation.

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Doubling time is 36 hours. Saturation density at confluence is 1x100,000 cells/cm2.
  Passages: +4; Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: DSMZ: ACC 219

Bibliographic references:

• Cancer Res 1992;52:1369 - PMID: 1737400
• Int J Oncol 1994;4:583-586

See homonymous cell lines in other collections and catalogues.