FM3Ats C1.T85 (original) (raw)

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FM3Ats C1.T85 (mouse, C3H, breast, carcinoma)

SPECIES: mouse, C3H
TISSUE/ORGAN: breast
TUMOR: carcinoma

ECACC catalog code: 87111904
Morphology: Lymphoblast
Description: Mouse C3H mammary carcinoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies, chromosome condensation studies.

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1-3x100,000 cells/ml; 5% CO2; 33C.
  A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (ECACC No. 87100804). Cells will Not double at 39C (Non-permissive temperature) and will grow at 33C (permissive temperature). Electron Microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39C for 16 hours. This abnormal condensation does Not occur at 33C or in the FM3A (ECACC No. 87100804) cell line at either temperature.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Exp Cell Res 1980;126:407-416 - PMID: 6153988