H69 (original) (raw)

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H69 (human, lung, carcinoma, small cell)

SPECIES: human
TISSUE/ORGAN: lung
TUMOR: carcinoma, small cell

ECACC catalog code: 91091802
Morphology: Spherical, floating aggregates
Description: Human Caucasian lung small cell carcinoma
Viruses: See Properties
Depositor: Dr R I Freshney, CRC Department of Medical Oncology, University of Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO2; 37C.
  Passages: 33; Karyotype: Aneuploid
  This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 119

Bibliographic references:

• Cancer Res 1985;45:2913-2923 - PMID: 2985257