HG261 (original) (raw)

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HG261 (human, Caucasian, skin, fibroblast, Fanconi anemia)

SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: Fanconi anemia (OMIM Home)

ECACC catalog code: 90112603
Morphology: Fibroblast
Susceptibility to viruses: herpes simplex * poliovirus 1 * SV40 * vaccinia * vesic. stomatitis (Indiana)
Description: Human Caucasian skin Fanconi's anaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome and virus studies: SV40

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Limited lifespan; up to 35 passages possible.
  Passages: 22; Karyotype: 2n = 46, diploid
  From 6 year old Caucasian male with Fanconi's anaemia which is associated with high incidence of leukaemia. Chromosomes show a tendency to spontaneous abnormalities including breaks and complex rearrangement. Have a higher proportion of transformed colonies with SV40 compared with normal diploid cells.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 122

Bibliographic references:

• Ann Genet (Paris) 1966;9:143
• Natl Cancer Inst Monogr 1968;29:271 - PMID: 4305429