HGC-27 (original) (raw)

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HGC-27 (human, lymph node, carcinoma)

SPECIES: human
TISSUE/ORGAN: lymph node
TUMOR: carcinoma

ECACC catalog code: 94042256
Morphology: Epithelial
Description: Human gastric carcinoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Products: Mucin

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Passages: 14; Undifferentiated, mucin producing.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: HTL95028

Bibliographic references:

• Acta Med Okayaues 1976;30:215

See homonymous cell lines in other collections and catalogues.