HT 1080 (original) (raw)

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HT 1080 (human, Caucasian, acetabulum, fibrosarcoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: acetabulum
TUMOR: fibrosarcoma

ECACC catalog code: 85111505
Morphology: Epithelial
Susceptibility to viruses: feline leukemia * poliovirus 1 * RD114 * vesic. stomatitis (Indiana)
Description: Human fibrosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and virus studies: RD114, FeLV

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Karyotype: 2n = 46, pseudodiploid
  Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosuppressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 and FeLV.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 121, IZSBS: BS TCL 27

Bibliographic references:

• Cancer 1974;33:1027-1033 - PMID: 4132053

See homonymous cell lines in other collections and catalogues.