HVS-SILVA 40 (original) (raw)

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HVS-SILVA 40 (monkey, marmoset, blood)

SPECIES: monkey, marmoset
TISSUE/ORGAN: blood

ECACC catalog code: 90020105
Morphology: Lymphoblast
TRANSFORMED BY: herpes saimiri strain II
Description: Monkey Marmoset lymphocytes, transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: T8 Antigen Applications: Transformation and virus studies: Herpesvirus saimiri strain II

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  IDMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37C. Sub-culturing is normally only required once per week.
  Established by infecting peripheral blood lymphocytes of a 5 year old, male marmoset (Callithrix jacchus) with Herpesvirus saimiri strain II. Cells express TS Ag and exhibit natural killer functions.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1773

Bibliographic references:

• J Exp Med 1986;164:926 - PMID: 3018122
• J Virol 1986;57:701 - PMID: 3003401