A10-85 (original) (raw)

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A10-85 (rat, BDIX, brain, glioma)

SPECIES: rat, BDIX
TISSUE/ORGAN: brain
TUMOR: glioma

ECACC catalog code: 93040117
Morphology: Epitheloid
Description: Rat glioma
Viruses: See Properties
Depositor: Dr J Roscoe, Department of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Experimental tumour models, carcinogenesis studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 52; Karyotype: Modal No 42
  The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.
  Research council deposit: Yes; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Br J Exp Pathol 1979;60:209 - PMID: 221002