A2058 (original) (raw)

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A2058 (human, skin, melanoma, metastatic)

SPECIES: human
TISSUE/ORGAN: skin
TUMOR: melanoma, metastatic

ECACC catalog code: 91100402
Morphology: Epithelial
Description: Human caucasian metastatic melanoma
Viruses: See Properties
Depositor: Dr M Simpkins,Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Nerve growth factor (NGF), Laminin

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
  Research council deposit: Yes; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: HTL08002

Bibliographic references:

• Ann N Y Acad Sci 1990;580:375-384 - PMID: 2159752
• Biochemistry 1993;32(6):1583-1592 - PMID: 8431437
• Cancer Res 1990;50(17):5581-5586 - PMID: 2201438
• Int J Cancer 1990;45(6):1137-1142 - PMID: 2161801
• Int J Cancer 1990;46(2):282-286 - PMID: 2166712
• Int J Cancer 1990;46(2):287-292 - PMID: 2116998
• Int J Cancer 1990;46(3):456-461 - PMID: 2394516
• Int J Cancer 1990;46(3):552-558 - PMID: 2394516
• J Biol Chem 1982;257(9):5220-5225 - PMID: 6279663
• J Biol Chem 1989;264(28):16652-16657 - PMID: 2550450
• J Biol Chem 1989;264(36):21544-21549 - PMID: 2557332
• J Biol Chem 1992;267(25):17743-17747 - PMID: 1387642
• J Cell Biol 1987;105(5):2409-2415 - PMID: 3680388
• J Cell Biol 1990;110(4):1427-1438 - PMID: 2324200
• Mol Endocrinol 1988;2(11):1056-1063 - PMID: 2464748
• Proc Natl Acad Sci U S A 1986;83(10):3302-3306 - PMID: 3085086

See homonymous cell lines in other collections and catalogues.