A375 (original) (raw)

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A375 (human, skin, malignant melanoma)

SPECIES: human
TISSUE/ORGAN: skin
TUMOR: malignant melanoma

ECACC catalog code: 88113005
Morphology: Epithelial
Description: Human malignant melanoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
  Passages: 172; Karyotype: Modal No = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
  Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.
  Research council deposit: No; Price Code: B;
  Validated by: chromosome analysis (normal N2, N6 and N22 present in single copies) * isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1619

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