Ampli-GPE (original) (raw)

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

Ampli-GPE (mouse, NIH Swiss, embryo)

SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo

ECACC catalog code: 95050229
Morphology: Fibroblast
Transfected by: Transfection Back To Transfection WebPage
Description: Mouse NIH Swiss embryo, transfected with retroviral DNA
Viruses: See Properties
Depositor: Dr Y Takahara, Central Research Laboratories, Ajinomoto Co., Kawasaki, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Retrovirus packaging, gene transfer assays

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 250�g/ml G418 + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: +3; The retrovirus packaging Ampli-GPE (clone A57) cells were obtained from NIH3T3 fibroblasts (ECACC No. 93061524) being co-transfected with two kinds of recombinant chimeric proviral genes in the BMG Neo vector. To reduce the homology between proviral DNA in the packaging cell and the retroviral vector, the gag-pol and env genes of Moloney murine leukaemia virus were separated onto two different plasmids in which the 5' long terminal repeat (LTR) and the 3' LTR had been replaced by the mouse metallothionein I promoter or the rabbit �-globin gene. This expression and amplification system produces a viral titre high enough for gene transfer to somatic cells (up to 5x1,000,000 CFU/ml) and avoids the generation of replication-competent viruses.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• J Virol 1992;66:3725 - PMID: 1316479