K1 (original) (raw)

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

K1 (human, thyroid, carcinoma)

SPECIES: human
TISSUE/ORGAN: thyroid
TUMOR: carcinoma

ECACC catalog code: 92030501
Description: Human thyroid carcinoma
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Wild-type p53 tumour suppressor gene Applications: Tumourigenicity studies, particularly the role of p53

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM: Ham's F12:MCDB 104 (2:1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Mol Carcinog 1993;7:83-88