LC540 (original) (raw)

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LC540 (rat, Fisher, testis, Leydig cells, testicular tumor)

SPECIES: rat, Fisher
TISSUE/ORGAN: testis, Leydig cells
TUMOR: testicular tumor

ECACC catalog code: 89031604
Morphology: Epithelial
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: Rat Fischer Leydig cell testicular tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
  Passages: 57; Karyotype: 2n = 42
  A cell line from a spontaneous transplantable Leydig cell tumour in a Fischer strain rat, reported to secrete steroid, however over a 6 hour period the secretion of oestradiol and testosterone by RIA could not be confirmed.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 43

Bibliographic references:

• Ann Meeting Soc Reprod Abst 1970;4
• J Natl Cancer Inst 1968;41:1141 - PMID: 5688092