LS174T (original) (raw)

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LS174T (human, colon, adenocarcinoma)

SPECIES: human
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma

ECACC catalog code: 87060401
Morphology: Epithelial
Description: Human Caucasian colon adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C May take up to 7 days for cells to attach.
  Passages: 116; Karyotype: Modal No. 45 (2n
  A trypsinised variant of LS180, both lines being derived from a 58 year old female with Dukes type B adenocarcinoma of the colon. The cells have micro villi and produce CEA and intracytoplasmic vacuoles. The cells are tumorigenic in nude mice.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 188

Bibliographic references:

• In Vitro 1976;12:180-191 - PMID: 1262041