MiCL1 (S+L-) (original) (raw)

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MiCL1 (S+L-) (mustela vison (mink), lung)

SPECIES: mustela vison (mink)
TISSUE/ORGAN: lung

ECACC catalog code: 86020603
Morphology: Fibroblast-like
TRANSFORMED BY: Moloney-MSV
Endogenous viruses: contains Moloney-MSV
Susceptibility to viruses: CCC feline primate virus * M7 feline primate virus * RD114 * type C * type C, feline leukemia * xenotropic murine leukemia
Description: Mink lung, Moloney murine sarcoma virus infected
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Passages: 17; Karyotype: 2n = 30
  A clone of the mink lung cell line Mv.1.Lu. Isolated leukaemia from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 64.1, ICLC: ATL97001

Bibliographic references:

• Science 1976;192:1143 - PMID: 179144
• Virology 1975;67:288 - PMID: 170737
• Virology 1975;67:344 - PMID: 52940

See homonymous cell lines in other collections and catalogues.