B6/FA (original) (raw)

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B6/FA (mouse, AKR, thymus, thymoma)

SPECIES: mouse, AKR
TISSUE/ORGAN: thymus
TUMOR: thymoma

ECACC catalog code: 94031008
Morphology: Spherical
Description: Mouse, AKR, thymus thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: To study the assembly and transport of Ly-2/3 antigen in the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC Class I molecules.

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C; saturation density 2x1,000,000 cells/ml.
  This Ly-2/3 negative cell line was derived from the parent cell line AKR1.G.1 OVAR.1.26
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Immunogenetics 1994;40:154 - PMID: 7517914
• J Immunol Methods 1992;154:211 - PMID: 1401954