NCI-H322 (original) (raw)

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NCI-H322 (human, Caucasian, lung, carcinoma, bronchioalveolar)

SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma, bronchioalveolar

ECACC catalog code: 95111734
Description: Human Caucasian bronchioalveolar carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 5-10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 101; NCI-H322 was derived in 1981 from a primary bronchioloalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 5806

Bibliographic references:

• Cancer Res 1990;50:5481 - PMID: 2386953