NMuMG (original) (raw)

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NMuMG (mouse, NAMRU, mammary gland)

SPECIES: mouse, NAMRU
TISSUE/ORGAN: mammary gland

ECACC catalog code: 94081121
Morphology: Epithelial
Description: Mouse mammary gland, normal epithelial
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: NMuMG cells express different integral surface proteoglycans (eg syndecan). They are used for studies of cell adhesion and growth factor-binding molecules as well as for analysis of growth factor effects on cell proliferation.

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Passages: 22; Initiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1636

Bibliographic references:

• J Natl Cancer Inst 1974;52:1375