Nthy-ts1 (original) (raw)

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Nthy-ts1 (human, thyroid, follicular)

SPECIES: human
TISSUE/ORGAN: thyroid, follicular

ECACC catalog code: 90011610
Morphology: Epithelial
Transfected by: Transfection Back To Transfection WebPage
Description: Human thyroid follicular mutant epithelial
Viruses: See Properties
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection, mutation and virus studies: SV40

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33C.
  Similar to Nthy-ori 3-1 (90011609) but was transfected with a temperature sensitive mutant encoding SV40 large T antigen. The cells grow at 33C.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Mol Cell Biol 1990;10:5365 - PMID: 1697930