PLC/PRF/5 (original) (raw)

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PLC/PRF/5 (human, liver, hepatoma)

SPECIES: human
TISSUE/ORGAN: liver
TUMOR: hepatoma

ECACC catalog code: 85061113
Morphology: Epithelial
Susceptibility to viruses: vesicular stomatitis
Description: Human liver hepatoma, Alexander cell line
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Hepatitis B Surface Antigen (HBsAg) Applications: Tumourigenicity and virus studies: Hepatitis B

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Alexander cell line which produces HBsAG. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 8024, IZSBS: BS TCL 122

Bibliographic references:

• J Virol 1979;32:796 - PMID: 92575
• Nature 1979;282:615-616 - PMID: 233137
• Proc Natl Acad Sci U S A 1985;82:83 - PMID: 2982146
• Science 1980;209:497-499 - PMID: 6248960
• Science 1983;222:385-389 - PMID: 6194563

See homonymous cell lines in other collections and catalogues.