RCE (original) (raw)

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

RCE (rabbit, New Zealand white, cornea)

SPECIES: rabbit, New Zealand white
TISSUE/ORGAN: cornea

ECACC catalog code: 95081046
Morphology: Epithelial-like
TRANSFORMED BY: SV40
Description: Rabbit corneal epithelial cells, SV40 transformed
Viruses: See Properties
Depositor: Dr K Araki-Sasaki, Department of Ophthalmology, Osaka University Medical School, Osaka, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Corneal epithelial research

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM:Ham's F12 (1:1) + 2mM Glutamine + 5�g/ml Insulin + 10ng/ml EGF + 0.1�g/ml Choleratoxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: +5; The RCE cell line, also known as K'RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation) Bibliographic references:
• Invest Ophthalmol Vis Sci 1993;34:2665