BF-F3 (original) (raw)

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BF-F3 (mouse / mouse, hybridoma)

SPECIES: mouse / mouse
TUMOR: hybridoma

DSMZ catalog code: ACC 212
Morphology: round cells growing singly in suspension
Description: mouse hybridoma (anti-rat fiber/myosin) this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgM) reacting with adult rat type 2B fibers in immunofluorescence and with rat type 2B MHCs (myosin heavy chain) in Western blotting; established in 1983 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles confirmed as mouse with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Prof. S. Schiaffino, University of Padova, Padova, Italy
General Restrictions

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS + 2 mM L-glutamine maintain at 0.2-0.5 x 106 cells/ml, optimal split ratio of 1:2 to 1:4 every 2-4 days at 37 C with 5% CO2 maximum density at about 1.0 x 106 cells/ml; doubling time of 20-30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 6.5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
  Validated by: cytogenetics 1 (murine chromosome morphology) * immunological analysis (secretion of a mouse IgM monoclonal antibody) * isoenzymes (confirmed as mouse with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative) Bibliographic references:
• J Muscle Res Cell Motil 1989;10:197-205 - PMID: 2547831