SIRC (original) (raw)

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SIRC (rabbit, cornea)

SPECIES: rabbit
TISSUE/ORGAN: cornea

ECACC catalog code: 89090404
Morphology: Epithelial
Susceptibility to viruses: N- and B-tropic oncornaviruses C * rubella
Description: Rabbit Cornea
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Rubella virus

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin 0.02% EDTA; 5% CO2, 37C.
  Karyotype: 2n = 44
  Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 60, IZSBS: BS CL79, ICLC: AL96001

Bibliographic references:

• Proc Soc Exp Biol Med 1966;122:783 - PMID: 5918951
• Proc Soc Exp Biol Med 1967;125:1271 - PMID: 6042441
• Science 1965;149:633 - PMID: 4378694
• Virology 1984;132:79-84 - PMID: 6607566

See homonymous cell lines in other collections and catalogues.