SW 1463 (original) (raw)

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SW 1463 (human, Caucasian, rectum, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: rectum
TUMOR: adenocarcinoma

ECACC catalog code: 90112713
Morphology: Epithelial
Description: Human Caucasian rectum adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  L-15 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; Medium change after 4-5 days. 37C. No CO2.
  Passages: 58; Karyotype: 2n = 46, hypertriploid
  Established from a grade II mucous producing and grade III solid rectal adenocarcinoma from a 66 year old Caucasian female. The cells form brush borders and produce CEA.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 234

Bibliographic references:

• Cancer Res 1976;36:4562-4569 - PMID: 1000501