BHK-21 (original) (raw)

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

BHK-21 (hamster, Syrian, kidney)

SPECIES: hamster, Syrian
TISSUE/ORGAN: kidney

DSMZ catalog code: ACC 61
Morphology: fibroblastoid adherent monolayer
Susceptibility to viruses: adenovirus 25 * herpes simplex * pseudorabies * reovirus 3 * rubella * vaccinia * vesic. stomatitis (Indiana)
Description: Syrian hamster kidney BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies confirmed as hamster with IEF of AST, LDH, MDH, MPI, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CCL 10), Rockville, Maryland, USA
General Restrictions

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% MEM (with Earle's salts) + 10% FBS + 1x non-essential amino acids split confluent culture 1:2 to 1:3 every 2-4 days using trypsin/EDTA; seed out at ca. 0.2 x 105 cells/cm 2 at 37C with 5% CO2 about 20-30 x 106 cells/80 cm 2; doubling time of ca. 32-50 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
  Validated by: cytogenetics 1 (near-diploid with 21% tetraploidy; 40-45,XY; hypetdiploid sideline present at 19%; resembles published description) * isoenzymes (confirmed as hamster with IEF of AST, LDH, MDH, NP, PEP B)

Availability in cell line catalogues: ATCC: CCL 10

Bibliographic references:

• J Natl Cancer Inst 1963;30:795-811
• Virology 1962;16:147-151
• Virology 1965;27:239
• Virology 1965;27:308