TK6 (original) (raw)

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TK6 (human, lymphoblast)

SPECIES: human
TISSUE/ORGAN: lymphoblast

ECACC catalog code: 95111735
Morphology: Suspension
Description: Human lymphoblast, thymidine kinase heterozygote
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,302,535
Properties: Applications: Bromodeoxyuridine resistant assays

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) or FBS. Maintain cultures between 1-9x100,000 cells/ml; 5% CO2; 37C.
  The thymidine kinase heterozygote cell line TK6 has been isolated from the lymphoblastoid line HH4. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. This cell line has been shown to be negative for Bovine Viral Diarrhoea Virus (BVDV).
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 8015

Bibliographic references:

• Biochem Biophys Res Commun 1978;84:411 - PMID: 718692