WES (original) (raw)

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WES (monkey, chimpanzee, skin)

SPECIES: monkey, chimpanzee
TISSUE/ORGAN: skin

ECACC catalog code: 91011612
Morphology: Fibroblast-like
TRANSFORMED BY: SV40
Description: Chimpanzee skin, SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and transformation studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:9 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 22; A non-producer, Adeno 12-SV40 transformed chimpanzee skin cell line. The cell line has a high saturation density, exhibits colony formation in soft agar, is morphologically altered and produces tumours in nude mice.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1609

Bibliographic references:

• Proc Natl Acad Sci U S A 1981;78:313 - PMID: 6264437