A4-840 (original) (raw)

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A4-840 (mouse / mouse, hybrid, hybridoma)

SPECIES: mouse / mouse
TISSUE/ORGAN: hybrid
TUMOR: hybridoma

DSMZ catalog code: ACC 213
Morphology: single round cells, lightly adherent
Description: mouse hybridoma (anti-fiber/myosin) described as secreting a mouse monoclonal antibody (IgM) reacting with slow muscle fibers in rodent, chicken and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. S. M. Hughes, King's College, London, England
General Restrictions

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids split saturated culture 1:5 every 3 days; seed out at about 0.3 x 106 cells/ml at 37C with 5% CO2 maximal density of about 1.5 x 106 cells/ml; doubling time of about 25 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
  Validated by: immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * reverse transcriptase (reverse transcriptase negative) Bibliographic references:
• Virchows Arch B Cell Pathol 1993;63:335-343