3T3-MSV (original) (raw)

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3T3-MSV (mouse, Swiss 3T3, embryo)

SPECIES: mouse, Swiss 3T3
TISSUE/ORGAN: embryo

ECACC catalog code: 90092701
Morphology: Fibroblast
TRANSFORMED BY: Moloney-MSV
Description: Mouse Swiss 3T3 embryo, Moloney-MSV transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Growth factor Applications: Cell growth studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
  Passages: 7; The cells do not produce virus particles. In serum-free media the cells release a specific growth factor. This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1568

Bibliographic references:

• Int J Cancer 1970;6:95 - PMID: 4319233
• Proc Natl Acad Sci U S A 1978;75:4001 - PMID: 211512