CHP 4 (W.W.) (original) (raw)

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CHP 4 (W.W.) (human, Black, skin, galactosemia)

SPECIES: human, Black
TISSUE/ORGAN: skin
PATHOLOGY: galactosemia*230400 (OMIM record)

ECACC catalog code: 90102537
Morphology: Fibroblast
Description: Human Negroid skin asymptomatic Galactosemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Enzyme deficiency

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
  Passages: 13; Karyotype: 2n = 46
  Derived from skin tissue taken from an 8 year old Negro male with asymptomatic galactosemia, despite lacking cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (ECACC No. 90102536) with the same enzyme deficiency whose was symptomatic. Cultures will retain characteristics for at least 30 passages
  Research council deposit: No; Price Code: C;

Availability in cell line catalogues: ATCC: CCL 133

Bibliographic references:

• Am J Med 1965;38:62