Clone 1-5c-4 (original) (raw)

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Clone 1-5c-4 (human, conjunctiva)

SPECIES: human
TISSUE/ORGAN: conjunctiva

ECACC catalog code: 88021103
Morphology: Epithelial with occasional giant cells
Susceptibility to viruses: influenza A * influenza B * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human conjunctiva, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Plaque assays, cloning, genetic and virus studies: swine influenza viruses

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  Medium 199 (HBSS) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using EDTA or 0.25% trypsin; 5% CO2; 37C.
  Passages: 73; Karyotype: 2n = 46
  Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival 'D' cells. Susceptible to swine influenza viruses, supports production of plaque formation by variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD This cell line was found to have a HeLa profile by DNA fingerprinting.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 20.2

Bibliographic references:

• In Vitro 1978;14:469-475 - PMID: 566722
• J Exp Med 1961;95:113
• Nature 1976;259:211-213 - PMID: 1250349
• Proc Soc Exp Biol Med 1954;87:440