COLO 853 (original) (raw)

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COLO 853 (human, axilla lymph node, melanoma)

SPECIES: human
TISSUE/ORGAN: axilla lymph node
TUMOR: melanoma

ECACC catalog code: 93052606
Morphology: Epithelial
Description: Human malignant melanoma
Viruses: See Properties
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Melanin Applications: Tumourigenicity studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Passages: +3; Derived from the right axilla lymph node of a 45 year male with melanoma.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: HTL96001

Bibliographic references:

• Cancer Genet Cytogenet 1993;69:108-112 - PMID: 8402545

See homonymous cell lines in other collections and catalogues.