CV-1 (original) (raw)

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CV-1 (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

ECACC catalog code: 87032605
Morphology: Fibroblast
Susceptibility to viruses: arbovirus * eastern equine encephalitis * herpes simplex * poliovirus 1 * SV40 * western equine encephalitis
Description: Monkey African Green kidney fibroblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
  Karyotype: 2n = 60, pseudodiploid
  Used in RSV transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African Green monkey kidney. Cells grow rapidly and form monolayers of fibroblast like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 70

Bibliographic references:

• Proc Natl Acad Sci U S A 1964;53:53
• Virology 1965;27:453 - PMID: 4285109

See homonymous cell lines in other collections and catalogues.